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Ultrastructural studies on the establishment, maintenance and breakdown of a coral-zooxanthella symbiotic system

Research Project

Project/Area Number 24570071
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionKochi University

Principal Investigator

OKUDA Kazuo  高知大学, 教育研究部総合科学系, 教授 (40152417)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsサンゴ / 褐虫藻 / 細胞共生 / 微細構造 / 微細形態
Outline of Final Research Achievements

Fine structure during the establishment, maintenance and breakdown of a coral-zooxanthella symbiotic system was examined. Suitable chemical and freeze fixation methods were applied for observing various cellular membranes clearly. When corals were treated with high temperatures, gastroderm cells without zooxanthellae autolyzed first, followed by the disconnection of cells with zooxanthellae away from gastroderm, so that the symbiotic system was broken down. Zooxanthellae in coral cells were found to have several distinct layers on the cortex. After cell divisions of zooxanthellae, symbiosome membranes and these layers were cleaved by constriction into two parts and enveloped the daughter cells. This process may ensure to distribute zooxanthellae into coral cells for the maintenance of the symbiotic system. Free-living zooxanthellae were attracted inward the gastric cavities of polyps in early developing corals and incorporated into gastroderm cells to establish the symbiotic systems.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Detection of characteristic heterochromatin distribution, highly amplified rRNA genes and presence of the human satellite III DNA motif in the scleractinian coral Echinophyllia aspera Ellis and Solander 17882014

    • Author(s)
      Taguchi T, Kubota S, Mezaki T, Sekida S, Okuda K, Nakachi S, Shinbo T, Iiguni Y and Tominaga A
    • Journal Title

      Chromosome Science

      Volume: 16 Pages: 33-38

    • NAID

      130004707283

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cytogenetic analysis of the scleractinian coral Acropora solitaryensis Veron & Wallace 19842014

    • Author(s)
      Taguchi T, Mezaki T, Iwase F, Sekida S, Kubota S, Fukami H, Okuda K, Shinbo T, Oshima S, Iiguni Y, Testa JR and Tominaga A
    • Journal Title

      Zoological Science

      Volume: 31 Pages: 89-94

    • NAID

      40019989087

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] サンゴに共生する褐虫藻の微細構造と生理学的挙動2012

    • Author(s)
      関田諭子・奥田一雄
    • Journal Title

      海洋と生物

      Volume: 34 Pages: 338-342

    • Related Report
      2012 Research-status Report
  • [Presentation] サンゴ細胞内における褐虫藻の細胞分裂の電子顕微鏡観察2014

    • Author(s)
      関田諭子、奥田一雄
    • Organizer
      日本サンゴ礁学会
    • Place of Presentation
      高知市高知城ホール
    • Year and Date
      2014-11-26 – 2014-12-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ribosomal RNA gene mapping and manifestation of sex chromosomes on the scleractinian coral Acropora Solitaryensis Veron & Wallace2014

    • Author(s)
      Taguchi T, Kubota S, Mezaki T, Sekida S, Okuda K, Tominaga A
    • Organizer
      10th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      Taipei, Taiwan
    • Related Report
      2013 Research-status Report
  • [Presentation] Ultrastructure of cortical and extracellular structures of zooxanthellae during cell division in a coral cell2013

    • Author(s)
      Sekida S, Okuda K
    • Organizer
      10th international phycological congress
    • Place of Presentation
      Orlando, Florida, USA
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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