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The mechanism of oocyte maturation in sea cucumber.

Research Project

Project/Area Number 24570072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionKyushu University

Principal Investigator

YOSHIKUNI Michiyasu  九州大学, (連合)農学研究科(研究院), 教授 (50210662)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords卵成熟 / 濾胞細胞 / 神経ホルモン / ニセクロナマコ / マナマコ / 排卵 / 卵黄タンパク質 / 卵黄蛋白質 / 卵黄形成 / 卵成熟誘起ホルモン / ホルモン / 生理活性物質 / 海洋資源 / 神経ペプチド
Outline of Final Research Achievements

The nerve tissue of sea cucumber contains a neuropeptide which stimulates ovarian follicle cells to secret a second hormone in inducing oocyte maturation and ovulation. The neuropeptide was purified from Holothuria leucospilota and identified its peptide sequence. The homologous peptides were also found in genomic data of Apostichopus japonicus and Holothuria scabra. These peptides may play as the common hormone in various sea cucumbers.
Coelomic fluid of Apostichopus japonicus contains a lot of small vesicles which are collected after ultracentrifugation. Analysis by SDS-polyacrylamide gel electrophoresis revealed that these vesicles contain the major yolk protein. These vesicles may be involved in the transport system of the yolk protein in sea cucumber.
The nerve tissue of Apostichopus japonicus may contain an activity which induces ovulation of fully grown oocytes without oocyte maturation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2014 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Invited: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] ハネジナマコの生殖腺と卵巣の周年変化2014

    • Author(s)
      南洋一、吉国通庸
    • Journal Title

      水産増殖

      Volume: 未定

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] マナマコにおける卵黄蛋白質の輸送機構2014

    • Author(s)
      Hoang Ding Chieu,吉国通庸
    • Organizer
      日本動物学会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Comprehensive Analysis of Neuropeptides of Marine Invertebrates2012

    • Author(s)
      Michiyasu Yoshikuni
    • Organizer
      12th International Congress of the International Society for Developmental & Comparative Immunology
    • Place of Presentation
      Fukuoka City
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 海産無脊椎動物の神経分泌ペプチドの網羅的解析2012

    • Author(s)
      吉国通庸
    • Organizer
      基礎生物学研究所研究会
    • Place of Presentation
      岡崎市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Neural peptides induce oocyte maturation and gamete spawning of sea cucumber, Holothuria scabra.2012

    • Author(s)
      Yoichi Minami and Michiyasu Yoshikuni
    • Organizer
      10th Japan-Korea, Korea-Japan Joint Symposium on Aquaculture
    • Place of Presentation
      Nagasaki City
    • Related Report
      2012 Research-status Report
  • [Book] 科研費NEWS2012

    • Author(s)
      吉国通庸
    • Total Pages
      23
    • Publisher
      文部科学省・日本学術振興会
    • Related Report
      2012 Research-status Report
  • [Book] ナマコ学 -生物・産業・文化- 第3章 成熟・産卵2012

    • Author(s)
      吉国通庸
    • Total Pages
      227
    • Publisher
      成山堂
    • Related Report
      2012 Research-status Report
  • [Remarks] 九州大学大学院農学研究院動物資源科学部門アクアフィールド科学分野吉国研究室

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/jikkensho/yoshikuni_lab/yoshikuni_top.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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