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Application of a unique 3-D reconstruction software unaffected by the missing data range to biological tomography

Research Project

Project/Area Number 24570076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionKogakuin University

Principal Investigator

BABA Misuzu  工学院大学, 付置研究所, 研究員 (80435528)

Co-Investigator(Renkei-kenkyūsha) BABA Norio  工学院大学, 情報学部, 教授 (80164896)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords電子顕微鏡 / 電子線CT / 酵母 / 急速凍結置換法 / 画像処理 / 位置合わせ / ニューラルネットワーク / 電子線トモグラフィ / テクスチャ解析
Outline of Final Research Achievements

Electron CT is widely used as an efficient technique for analyzing three-dimensional intracellular membrane structures using electron microscopy. This is because three-dimensional observation enables us to capture the connection among membrane structures even though they appear to be separated on the two-dimensional images. At the present, for this technique, manual control in the foreign-made software is widely used. However, such manually operated work process is a major problem for many researchers that enormous energy and time are spent. In this study, we developed a new elemental technology to avoid such a problem. We succeeded to develop a software that enables automation and increase in speed for Electron CT analysis, resulting in reducing time and improvement of accuracy of the work.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A Role for the Ancient SNARE Syntaxin 17 in Regulating Mitochondrial Division2015

    • Author(s)
      K. Arasaki, H. Shimizu, H. Mogari, N. Nishida, N. Hirota, A. Furuno, Y. Kudo, M. Baba, N. Baba, J. Cheng, T. Fujimoto, N.Ishihara, C. Ortiz-Sandoval, L. Barlow, A Raturi, N. Dohmae, Y. Wakana, H. Inoue, K.Tani, J. Dacks, T.Simmen, M. Tagaya
    • Journal Title

      Developmental Cell

      Volume: 32(9) Issue: 3 Pages: 304-317

    • DOI

      10.1016/j.devcel.2014.12.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alternative automatic alignment method for specimen tilt-series images based on back-projected volume data cross-correlations2014

    • Author(s)
      S. Tomonaga, M. Baba and N. Baba
    • Journal Title

      Microscopy

      Volume: 63(4) Issue: 4 Pages: 279-294

    • DOI

      10.1093/jmicro/dfu014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A new field-of-view auto tracking method for online tomography reconstruction based on back-projected ray image cross-correlation2014

    • Author(s)
      S. Tomonaga, M. Baba, S. Yamazaki and N. Baba
    • Journal Title

      Microscopy

      Volume: 63(5) Issue: 5 Pages: 357-369

    • DOI

      10.1093/jmicro/dfu021

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Alternative automatic alignment method for specimen tilt-series images based on back-projected volume data cross-correlations.2014

    • Author(s)
      Sachihiko Tomonaga, Misuzu Baba, and Norio Baba
    • Journal Title

      Microscopy

      Volume: -

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A role for Atg8-PE deconjugation in autophagosome biogenesis.2012

    • Author(s)
      Nair U, Yen WL, Mari M, Cao Y, Xie Z, Baba M, Reggiori F, Klionsky DJ
    • Journal Title

      Autophagy

      Volume: May 1;8(5) Issue: 5 Pages: 780-793

    • DOI

      10.4161/auto.19385

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Guideline for the use and interpretation of assays for monitoring autophagy2012

    • Author(s)
      Klionsky DJ,et al
    • Journal Title

      Autophagy

      Volume: 8 Issue: 4 Pages: 445-544

    • DOI

      10.4161/auto.19496

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 逆投影線像3次元相互相関法による投影像シリーズの倍率補正2015

    • Author(s)
      杉本健太、友永祥彦、馬場美鈴、馬場則男
    • Organizer
      日本顕微鏡学会第39回関東支部講演会
    • Place of Presentation
      工学院大学
    • Year and Date
      2015-02-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] A new field-of-view autotracking method based on back-projected ray image cross-correlation for online tomography reconstruction2014

    • Author(s)
      Sachihiko Tomonaga, Misuzu Baba and Norio Baba
    • Organizer
      日本顕微鏡学会第58 回シンポジウム
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2014-11-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 従来法に代わる3次元断層データ相関法に基づく投影像の位置合わせ法2014

    • Author(s)
      友永祥彦,馬場 美鈴,馬場 則男
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2014-05-12 – 2014-05-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高精細位相シフトPCMによる倍率等の投影像シリーズ画像補正の自動化応用2014

    • Author(s)
      杉本健太,友永祥彦,馬場美鈴,馬場 則男
    • Organizer
      日本顕微鏡学
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2014-05-12 – 2014-05-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 酵母細胞のphagophore膜形成に関与する新規膜構造体の検出2013

    • Author(s)
      馬場 美鈴, 友永 祥彦, 鈴木 雅斗, 加藤 大貴, 馬場 則男
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2013 Research-status Report
  • [Presentation] 従来法に代わる断層像相関法に基づく投影像の位置合わせ法2013

    • Author(s)
      友永 祥彦, 加藤 大貴, 馬場 美鈴, 馬場 則男
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2013 Research-status Report
  • [Presentation] テクスチャ解析とスネーク法の改良による電子線CTセグメンテーション法2013

    • Author(s)
      鈴木 雅斗, 馬場 美鈴, 馬場 則男
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2013 Research-status Report
  • [Presentation] An Alternative Image Alignment Method Based on Reconstructed Cross-section Image Correlations2013

    • Author(s)
      Tomonaga S, Kato D, Baba M, and Baba N
    • Organizer
      Microscopy and Microanalysis 2013 Meeting,
    • Place of Presentation
      Indianapolis, convention center
    • Related Report
      2013 Research-status Report
  • [Presentation] 動的閾値処理による電子線CT セグメンテーション法2012

    • Author(s)
      鈴木雅斗,馬場 美鈴, 馬場 則男
    • Organizer
      第68回日本顕微鏡学会学術講演会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 従来法に代わる断層像画質評価に基づく投影像の位置合わせ法2012

    • Author(s)
      友永祥彦,加藤大貴, 馬場 美鈴,馬場 則男
    • Organizer
      第68回日本顕微鏡学会学術講演会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Morphological analysis of the intracellular structures in atg Mutants2012

    • Author(s)
      M. Baba, M. Suzuki, S. Tomonaga, M. Ogasawara, Y. Kamada and N. Baba
    • Organizer
      The 6th Int. Symposium on Autophasy
    • Place of Presentation
      Bankoku Shinryokan
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 荷電粒子線装置、三次元画像の再構成画像処理システム、方法2014

    • Inventor(s)
      馬場則男,友永祥彦, 馬場美鈴, 橋本隆仁, 四辻貴文
    • Industrial Property Rights Holder
      (株)日立ハイテクノロジーズ
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-06-10
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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