| Project/Area Number |
24570077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIKUYAMA Sakae 早稲田大学, 教育・総合学術院, 名誉教授 (20063638)
KOBAYASHI Tetsuya 埼玉大学, 大学院理工学研究科, 教授 (00195794)
HASUNUMA Itaru 東邦大学, 理学部, 講師 (40434261)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 抗菌ペプチド / 生体防御ペプチド / 両生類 / ハーダー腺 / カセリシジン / ヒストン / DT40細胞 / cathelicidin / LAH-2細胞 / リュウキュウアカガエル / catesbeianalectin / ウシガエル / 抗酸化ペプチド |
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| Outline of Final Research Achievements |
We have cloned cDNAs encoding antimicrobial peptide (AMP) precursors from total RNA samples prepared from amphibian skin, Harderian gland, and brain and cells of the avian cell line DT40 using RT-PCR techniques. We detected chemotactic and degranulation against mastocytoma, bacterial surface substance-binding, and/or lectin-like activities as well as antimicrobial activities in the synthetic replicates of these AMPs such as bullfrog catesbeianalectin (CL) and chicken cathelicidin-B1 (chCATH-B1). We also detected the bacterial toxins-inducible chCATH-B1gene expression in DT40 cells. In addition, we showed directed antimicrobial activities and properties of Arg-rich histones (H3 and H4) and Lys-rich histone (H2B) against Gram-positive bacterium Staphylococcus aureus. While the Arg-rich histones disrupted the bacterial cell membrane by forming blebs, the Lys-rich histone inhibited the bacterial cell growth without morphological changes.
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