| Project/Area Number |
24570078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HASEBE Takashi 日本医科大学, 医学部, 准教授 (70329027)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 小腸 / 幹細胞 / 上皮脱分化 / 甲状腺ホルモン / 変態 / アフリカツメガエル / ニッチ / シグナル伝達経路 / 器官培養 / Wnt |
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| Outline of Final Research Achievements |
In the amphibian digestive tract, some of larval epithelial cells dedifferentiate into adult stem cells by the inductive action of thyroid hormone (TH). In the present study, to clarify molecular mechanisms underlying the epithelial dedifferentiation into the stem cells, we performed expression and functional analyses of TH response genes, using the culture system for the Xenopus laevis small intestine. Our results indicate that TH-activated Wnt5a/Ror2 signaling is essential for the larval epithelial dedifferentiation accompanied by changes in cell morphology. In addition, our findings strongly suggest that absorptive epithelial cells expressing Ror2, which are scattered in the larval epithelium, can dedifferentiate into the stem cells. To clarify the origin of the stem cells, we isolated the promoter of Ror2 and started to generate transgenic frogs for lineage analysis of the epithelial cells expressing Ror2.
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