Identification of neural circuitry controlling the courtship song of Drosophila

• Project/Area Number: 24570082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Tohoku University
• Principal Investigator: KOGANEZAWA Masayuki 東北大学, 生命科学研究科, 准教授 (10302085)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Daisuke 東北大学, 大学院生命科学研究科, 教授 (50318812)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ショウジョウバエ / 求愛行動 / fruitless / doublesex

Outline of Final Research Achievements

The sexual differentiation of somatic cells in Drosophila is controlled by the terminal genes in the sex determination hierarchy; fruitless (fru) and doublesex (dsx). Recent studies revealed that neurons expressing these genes play central role in the courtship behavior. The courtship song produced by a male fly is one of the hallmarks of courtship of Drosophila. To reveal the neural basis for courtship song production, I performed behavioral experiments by using the technique to manipulate neural activity of fru/dsx-expressing neurons. In this study, I identified 4 types of dsx-expressing neurons controlling proper courtship song production.

Research Products

• [Journal Article] The Drosophila Lingerer protein cooperates with Orb2 in long-term memory formation. (2014)
  • Author(s): Kimura, S., Sakakibara, Y., Sato, K., Ote, M., Ito, H., Koganezawa, M., and Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 1 Pages: 8-17
  • DOI: 10.3109/01677063.2014.917644
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Neuroethology of male courtship in Drosophila: from the gene to behavior. (2014)
  • Author(s): Yamamoto D., Sato K., and Koganezawa M.
  • Journal Title: J. Comp. Physiol. A. Volume: 200 Issue: 4 Pages: 251-264
  • DOI: 10.1007/s00359-014-0891-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Select interneuron clusters determine female sexual receptivity in Drosophila (2013)
  • Author(s): Sakurai, A., Koganezawa, M., Yasunaga, K., Emoto, K. and Yamamoto, D.
  • Journal Title: Nat. Commun. Volume: 4 Pages: 1825-1833
  • Peer Reviewed
• [Journal Article] Genes and circuits of courtship behavior in Drosophila males. (2013)
  • Author(s): Yamamoto, D. and Koganezawa, M.
  • Journal Title: Nat. Rev. Neurosci. Volume: 14 Issue: 10 Pages: 681-692
  • DOI: 10.1038/nrn3567
  • Peer Reviewed
• [Journal Article] Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism. (2012)
  • Author(s): Ito, H., Sato, K., Koganezawa, M., Ote, M., Matsumoto, K., Hama, C., Yamamoto, D.
  • Journal Title: Cell Volume: 149 Issue: 6 Pages: 1327-1338
  • DOI: 10.1016/j.cell.2012.04.025
  • Peer Reviewed
• [Presentation] Pheromone sensitive interneurons switching courtship and aggression in Drosophila. (2014)
  • Author(s): Koganezawa, M., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Sex-specific functions of longitudinals lacking. (2014)
  • Author(s): Sato, K., Toba, G., Ito, H., Koganezawa, M., Binglong, Z., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Functional characterization of the calcium channel in Drosophila embryonic muscles. (2014)
  • Author(s): Hara, Y., Koganezawa, M., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Neuropeptide-expressing neurons regulate sexual receptivity in Drosophila females. (2014)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K. and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Searching for neurons that regulate female sexual receptivity in Drosophila. (2014)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K., and Yamamoto, D.
  • Organizer: The 37th Annual Meeting of the Molecular Biology society of Japan
  • Place of Presentation: Kanagawa
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ショウジョウバエ脳神経系の雄化因子Fruitlessがもつ転写調節以外の機能 (2014)
  • Author(s): 佐藤耕世, 鳥羽岳太, 伊藤弘樹, 小金澤雅之, 山元大輔
  • Organizer: 第6回新学術領域研究「性差構築の分子基盤」領域会議
  • Place of Presentation: 舘山寺サゴーロイヤルホテル
  • Year and Date: 2014-10-09 – 2014-10-11
• [Presentation] ショウジョウバエ脳神経系の雄化因子Fruitless がもつ転写調節以外の機能 (2014)
  • Author(s): 佐藤耕世，鳥羽岳太，伊藤弘樹，小金澤雅之，山元大輔
  • Organizer: 日本動物学会第85回仙台大会
  • Place of Presentation: 東北大学川内キャンパス
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] doublesex-expressing neurons controlling female reproductive behavior in Drosophila. (2014)
  • Author(s): Kimura, K., Sato, C., Koganezawa, M., and Yamamoto D.
  • Organizer: 11th International Congress of Neuroethology (ICN)
  • Place of Presentation: Sapporo
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Neural circuitry switching between courtship and aggression in Drosophila. (2014)
  • Author(s): Koganezawa, M., and Yamamoto, D.
  • Organizer: 11th International Congress of Neuroethology (ICN)
  • Place of Presentation: Sapporo
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] ショウジョウバエの求愛／攻撃の切り替えに関わるfruitless発現神経回路 (2014)
  • Author(s): 小金澤雅之, 山元大輔
  • Organizer: 平成26年度日本動物学会東北支部大会
  • Place of Presentation: 岩手大学復興祈念銀河ホール
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] Sex-specific functions of longitudinals lacking (2013)
  • Author(s): Sato, K., Toba, G., Ito, H., Koganezawa, M. and Yamamoto, D.
  • Organizer: Neurobiology of Drosophila, Cold Spring Harbor Laboratory
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, USA
• [Presentation] ショウジョウバエの求愛/攻撃を制御する脳内fruitless発現ニューロン (2013)
  • Author(s): 小金澤雅之，山元大輔
  • Organizer: 日本動物学会 第84回年会
  • Place of Presentation: 岡山大学津島キャンパス，岡山
• [Presentation] 強制活性化法を用いた求愛と攻撃を制御する中枢ニューロンの同定 (2013)
  • Author(s): 小金澤雅之,山元大輔
  • Organizer: 平成25年度日本動物学会 東北支部大会
  • Place of Presentation: 秋田大学手形キャンパス・大学会館クレール，秋田
• [Presentation] ショウジョウバエの求愛と攻撃を制御するフェロモン感受性介在ニューロンの同定 (2013)
  • Author(s): 小金澤雅之，山元大輔
  • Organizer: 日本味と匂学会 第47回大会
  • Place of Presentation: 仙台市民会会館，宮城
• [Presentation] 雌キイロショウジョウバエの産卵管突き出し行動に関与するdsx発現ニューロン (2013)
  • Author(s): 佐藤千晶，小金澤雅之，山元大輔，木村賢一
  • Organizer: 日本動物学会 第84回年会
  • Place of Presentation: 岡山大学津島キャンパス，岡山
• [Presentation] ショウジョウバエにおける“種特異的”求愛行動パターンを生み出す神経基盤 (2013)
  • Author(s): 小金澤雅之
  • Organizer: 第6回Evo-devo青年の会「新奇性の生まれるとき The Emergence of Evoltionary Novelities」
  • Place of Presentation: 東京大学三崎臨海実験所、神奈川
  • Invited
• [Presentation] Functional dissection of fru/dsx-expressing neural circuitry generating Drosophila courtship behavior (2012)
  • Author(s): Koganezawa, M., Yamamoto, D.
  • Organizer: The 34th Annual Meeting of the Japanese Society of Comparative Physiology and Biochemistry
  • Place of Presentation: Shonan Village Center, Kanagawa
• [Presentation] ショウジョウバエ種特異的求愛行動パターンを生み出すfru発現神経回路 (2012)
  • Author(s): 小金澤雅之、山元大輔
  • Organizer: 平成24年度日本動物学会東北支部大会
  • Place of Presentation: 山形大学理学部、山形
• [Presentation] ショウジョウバエdoublesex発現ニューロン群における性差－1．性差形成機構 (2012)
  • Author(s): 木村賢一、佐藤千晶、小金澤雅之、山元大輔
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学、豊中
• [Presentation] ショウジョウバエdoublesex発現ニューロン群における性差－2．雌生殖行動の制御 (2012)
  • Author(s): 佐藤千晶、小金澤雅之、山元大輔、木村賢一
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学、豊中
• [Presentation] ショウジョウバエ求愛歌を制御するfruitless/doublesex発現神経回路 (2012)
  • Author(s): 小金澤雅之、山元大輔
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学、豊中
• [Presentation] Functional dissection of neural circuitry generating Drosophila courtship song by intersectional approach (2012)
  • Author(s): Koganezawa, M., Yamamoto, D.
  • Organizer: The 35th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya Congress Center, Nagoya
• [Presentation] ショウジョウバエ求愛行動を司るfruitless発現神経回路 (2012)
  • Author(s): 小金澤雅之
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部、福岡
  • Invited
• [Presentation] Electrophysiological properties of Drosophila embryonic body-wall muscles and their synaptic regulation (2012)
  • Author(s): Yokoyama, S., Yoshihara, M., Koganezawa, M., Yamamoto, D.
  • Organizer: Japanese Drosophila Research Conference 2012
  • Place of Presentation: Jikei University School of Medicine, Tokyo
• [Presentation] Indetification of neurons controlling female reproductive behavior in Drosophila (2012)
  • Author(s): Sato, C., Koganezawa, M., Yamamoto, D., Kimura, K.-I.
  • Organizer: Japanese Drosophila Research Conference 2012
  • Place of Presentation: Jikei University School of Medicine, Tokyo
• [Book] Insect pheromone behavior: fruit fly. Methods. Mol. Biol. 1068, 261-272. In: Pheromone Signaling, Ed. K. Tohara (2013)
  • Author(s): Yamamoto, D., Kohatsu, S. and Koganezawa, M.
  • Total Pages: 399
  • Publisher: Humana Press, Springer
• [Book] in vivo optical recording of brain interneuron activities from a Drosophila male on a treadmill. In: Genetically Encoded Functional Indicators, J.-R. Martin Ed. (2012)
  • Author(s): Kohatsu, S., Koganezawa, M., Yamamoto, D.
  • Publisher: Humana Press Springer: New York