Identification and functional analyses of genes expressed in the nerve ring of Hydra
| Project/Area Number |
24570089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Fukuoka Women's University |
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Principal Investigator |
HAMADA Shun 福岡女子大学, 文理学部, 教授 (60282349)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Kayoko 福岡女子大学, 国際文理学部, 研究員 (20448814)
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| Research Collaborator |
BOSCH Thomas, C. G. キール大学(ドイツ), 動物学研究所, 教授
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 神経生物学 / 進化生物学 / シナプス / 刺胞動物 / 神経回路 / 国際情報交換 / 神経生物 / 神経科学 / 散在神経系 / 神経回路形成 / ドイツ / 神経環 |
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| Outline of Final Research Achievements |
The diffuse nervous system (DNS) is considered as the most primitive form of the nervous system. Though the DNS is generally believed to be homogenous neuronal network, conspicuous nerve bundles with concentration of neurons have been reported. These structures would be the phylogenetically oldest centralization of the nervous system. In this study, we focused on a nerve bundle around the mouth of Hydra, the nerve ring. We screened for genes that are expressed in the nerve ring of Hydra by differential cDNA cloning followed by in situ hybridization. Among the candidate genes, we confirmed that Hydra synapsin (HySyn) was expressed in the nerve ring. Interestingly, HySyn was detected in subpopulations of neurons including the nerve ring in the Hydra DNS. At the ultrastructural level, HySyn was detected with clusters of synaptic vesicles. Ectopic expression of HySyn-GFP in neurons which do not express detectable level of HySyn affected their neurite morphology.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Thiamine deficiency induces massive cell death in the olfactory bulbs of mice.2013
Author(s)
Hamada, S., Hirashima, H., Imaeda, M., Okamoto, Y., Hamaguchi-Hamada, K., Kurumata-Shigeto, M.
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Journal Title
Journal of Neuropathology & Experimental Neurology
Volume: 72
Issue: 12
Pages: 1193-1202
DOI
Related Report
Peer Reviewed
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[Presentation] Distribution pattern of hydra synapsin revealed heterogeneity of synapses in the diffuse nervous system.2015
Author(s)
Hamada, S., Shigeto, M., Hamada, K., Minobe,S., Khalturin, K., Koizumi, O., Bosch, T.
Organizer
The 120th Annual Meeting of The Japanese Association of Anatomists and The 92th Annual Meeting of The Physiological Society of Japan
Place of Presentation
神戸国際展示場
Year and Date
2015-03-23
Related Report
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[Presentation] Origin and Evolution of the Central Nervous System: The Nerve Ring of Cnidarians.2013
Author(s)
Koizumi, O., Hamada, S., Minobe, S., Hamaguchi-Hamada, K., Kurumata-Shigeto, M.
Organizer
The 8th International Conference on Coelenterate Biology.
Place of Presentation
Eilat, イスラエル
Related Report
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[Presentation] Synthesis and Behavioral Activity of Short Chain Analogs of a Peptide Expected from hmp4846 Gene Expressed in Hydra.2013
Author(s)
Yamaguchi, Y., Nishi, M., Minobe, S., Hamada, S., Hamaguchi-Hamada, K., Koizumi, O., Sato, K.
Organizer
4th Asia-Pacific International Peptide Symposium (APIPS)
Place of Presentation
大阪
Related Report
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[Presentation] Functional analyses of a novel gene hmp4846 expressed in a sub-population of hydra nerve cells.2013
Author(s)
Minobe, S., Hamaguchi-Hamada, K., Yamaguchi, Y., Khalturin, K., Bosch, T.C.G., Sato, K., Koizumi, O., Hamada, S.
Organizer
The International Workshop “Unravelling the Developmental Regulatory Network in Early Animals"
Place of Presentation
Tutzing, ドイツ
Related Report
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[Presentation] Functional analyses of a novel gene expressed in a sub-population of hydra nerve net, hmp4846.2013
Author(s)
Minobe, S., Hamaguchi-Hamada, K., Yamaguchi, Y., Sato, K., Koizumi, O., Hamada, S.
Organizer
The 35th Annual Meeting of the Japanese Society for Comparative Physiology and Biochemistry
Place of Presentation
姫路
Related Report
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[Presentation] Functional analyses of a novel gene expressed in a sub-population of hydra nerve net, hmp48462012
Author(s)
Sumiko Minobe, Shun Hamada, Kayoko Hamaguchi-Hamada, Shiori Isomura, Konstantin Khalturin, Kazuki Sato, Thomas C.G. Bosch and Osamu Koizumi
Organizer
9th Okazaki Biology Conference, Marine Biology II
Place of Presentation
Okazaki Conference Center (Okazaki, Aichi), OIST Seaside House (Onna-son, Okinawa)
Related Report
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