Morphological and genetic analysis on isolation and maintain mechanisms of different corolla tube length using interspecific hybrid population

• Project/Area Number: 24570101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: The University of Tokushima
• Principal Investigator: YAMASHIRO Tadashi 徳島大学, 大学院ソシオ・アーツ・アンド・サイエンス研究部, 准教授 (50380126)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 種間交雑 / 花冠長 / 遺伝子型 / 雑種指数 / 生殖的隔離 / マイクロサテライト / ヤマハッカ属 / シソ科植物 / アキチョウジ / ヤマハッカ / 交雑集団

Outline of Final Research Achievements

An interspecific hybrid population between Isodon logitubus and I. japonicus was investigated by morphometric and genetic analyses. Both morphometric analyses and joint estimation of ancestry and interclass heterozygosity based on the SSR markers revealed that the F2 and backcross with I. longitubes were dominant in the population. Strong incompatibility was observed between I. longitubus and I. japonicus from artificial cross experiments. Although bumblebee pollinated the flowers of I. longitubus and hybrids, those of I. japonicus seem to be failed. Asymmetrical structure of the hybrid population (toward to I. longitubus) might be caused by the mismatch of the body size and I. japonicus inflorescence.

Research Products

• [Journal Article] Development of Microsatellite Markers for Isodon longitubus (Lamiaceae). (2013)
  • Author(s): Yamashiro T., Yamashiro A., Dohzono I. and Maki M.
  • Journal Title: Applications in Plant Sciences Volume: 1 Issue: 10 Pages: 1300028-1300028
  • DOI: 10.3732/apps.1300028
  • Peer Reviewed
• [Presentation] タカクマヒキオコシの花筒長変異と遺伝的分化 (2015)
  • Author(s): 荻嶋美帆,堂囿いくみ,星野佑介,山城考,堀江佐知子,牧雅之
  • Organizer: 日本生態学会
  • Place of Presentation: 「鹿児島大学郡元キャンパス（鹿児島県鹿児島市）」
  • Year and Date: 2015-03-19