Genetic differentiation and gene flow between populations of Cladophora species distributed in marine, brackish water or freshwater

• Project/Area Number: 24570106
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Fukui Prefectural University
• Principal Investigator: KAMIYA Mitsunobu 福井県立大学, 海洋生物資源学部, 教授 (00281139)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 汽水 / 塩濃度勾配 / 種分化 / 生理生態 / 雑種強勢 / 無性生殖 / ヘテロ接合度 / 地理的隔離 / 塩濃度 / 生理特性 / 遺伝子流動 / 倍数性 / アポミクシス / 着生

Outline of Final Research Achievements

Marine Cladophora albida included a larger number of widely-distributed ribotypes than marine C. opaca. Cladophora albida grows throughout the year, whereas C. opaca disappears during summer, which may affect on the frequency of interpopulational gene flow. Cladophora glomerata consisted of two phylogenetic groups; group 1 appeared in only freshwater habitats and the rate of heterozygous specimens was 7%, whereas group 2 was distributed in both freshwater and brackish habitats and all specimens were heterozygous. These results suggest that the two groups are not only reproductively isolated but also ecophysiologically differentiated.

Research Products

• [Journal Article] Diversity of phlorotannin profiles among sargassasacean species affecting variation and abundance of epiphytes. (2016)
  • Author(s): Nakajima, N., Sugimoto N., Ohki, K. & Kamiya, M.
  • Journal Title: European Journal of Phycology Volume: 未定 Issue: 3 Pages: 307-316
  • DOI: 10.1080/09670262.2016.1159340
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Defense mechanisms of sargassacean algae against epiphytic red alga Neosiphonia harveyi. (2015)
  • Author(s): Nakajima, N., Ohki, K. & Kamiya, M.
  • Journal Title: Journal of Phycology Volume: 51 Issue: 4 Pages: 695-705
  • DOI: 10.1111/jpy.12311
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Caloglossa beccarii (Delesseriaceae, Rhodophyta) from freshwater rivers in Kerala, India, a critical new record (2015)
  • Author(s): West, J. A., Kamiya, M., Ganesan, E. K., Louiseaux-de Goër, S. & Jose L.
  • Journal Title: Algae Volume: 30 Issue: 3 Pages: 207-216
  • DOI: 10.4490/algae.2015.30.3.207
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 富士北麓、精進湖と本栖湖におけるフジマリモの発見（予報） (2015)
  • Author(s): 芹澤（松山）和世・金原昂平・米谷雅俊・渡邊広樹・白澤直敏・田口由美・神谷充伸・芹澤如比古
  • Journal Title: 富士山研究 Volume: 9 Pages: 1-6
  • Open Access
• [Journal Article] Photoperiodic regulation of receptacle initiation in Sargassum horneri (Phaeophyceae) using clonal thalli. (2014)
  • Author(s): Yoshikawa, S., Ohki, K. & Kamiya, M.
  • Journal Title: Phycological Research Volume: 52 Issue: 3 Pages: 206-213
  • DOI: 10.1111/pre.12052
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cryptic diversity in the euryhaline red alga Caloglossa ogasawaraensis (Delesseriaceae, Ceramiales). (2014)
  • Author(s): Kamiya, M. & West, J. A.
  • Journal Title: Phycologia Volume: 53 Issue: 4 Pages: 374-382
  • DOI: 10.2216/13-242.1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High heterozygosity and phenotypic variation of zoids in apomictic Ulva prolifera (Ulvophyceae) from brackish environments. (2014)
  • Author(s): Ogawa, T., Ohki, K. & Kamiya, M.
  • Journal Title: Aquatic Botany Volume: 120 Pages: 185-192
  • DOI: 10.1016/j.aquabot.2014.05.015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of silicon-limitation on growth and morphology of Triparma laevis NIES-2565 (Parmales, Heterokontophyta) (2014)
  • Author(s): Kazumasa Yamada, Shinya Yoshikawa, Mutsuo Ichinomiya, Akira Kuwata, Mitsunobu Kamiya and Kaori Ohki
  • Journal Title: PloS ONE Volume: 9 Issue: 7 Pages: e103289-e103289
  • DOI: 10.1371/journal.pone.0103289
  • Peer Reviewed / Open Access
• [Journal Article] 異なる塩濃度環境に生育するシオグサ類の遺伝的多様度と分布パターンの比較 (2013)
  • Author(s): 丹羽一夫・早川雄一郎・神谷充伸
  • Journal Title: 藻類 Volume: 61 Pages: 74-80
  • NAID: 10031184313
  • Peer Reviewed
• [Journal Article] Observations on some mangrove-associated algae from the western Pacific (Guam, Chuuk, Kosrae, and Pohnpei). (2013)
  • Author(s): 34. West, J. A., Kamiya, M., de Goër, S. L., Karsten, U. & Zuccarello, G. C.
  • Journal Title: Algae Volume: 28 Pages: 241-266
  • Peer Reviewed
• [Journal Article] Difference of spatial distribution and seasonal succession among Ulva species (Ulvophyceae) across salinity gradients. (2013)
  • Author(s): Ogawa, T., Ohki, K. & Kamiya, M.
  • Journal Title: Phycologia Volume: 52 Pages: 637-651
  • Peer Reviewed
• [Journal Article] Species differentiation of defense ability against epiphyte Neosiphonia harveyi (Rhodomelaceae, Rhodophyta) in four sargassacean algae. (2013)
  • Author(s): Yamamoto, K., Endo, H., Yoshikawa, S., Ohki, K. & Kamiya, M.
  • Journal Title: Aquatic Botany Volume: 105 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Unique distribution of epiphytic Neosiphonia harveyi (Rhodomelaceae, Rhodophyta) along sargassacean hosts. (2012)
  • Author(s): Yamamoto, K., Yoshikawa, S., Ohki, K. & Kamiya, M.
  • Journal Title: Phycological Research Volume: 60 Pages: 70-75
  • NAID: 10030356919
  • Peer Reviewed
• [Journal Article] New records of red algae from mangroves in El Salvador and Pacific Mexico, combining culture and molecular observations. (2012)
  • Author(s): Zuccarello, G. C, Kamiya, M., Ootsuki, R., Loiseaux de Goer, S., Pedroche, F. F. and West, J. A.
  • Journal Title: Botanica Marina Volume: 55 Pages: 101-111
  • Peer Reviewed
• [Journal Article] Genetic and ecophysiological diversity of Cladophora (Cladophorales, Ulvophyceae) in various salinity regimes. (2012)
  • Author(s): Hayakawa, Y., Ogawa, T., Yoshikawa, S., Ohki, K. & Kamiya, M.
  • Journal Title: Phycological Research Volume: 60 Pages: 86-97
  • NAID: 10031136625
  • Peer Reviewed
• [Journal Article] Characterization of pico-cyanobacteria isolated from the halocline of the saline meromictic lake, Lake Suigetsu, Japan (ME11243). (2012)
  • Author(s): Ohki, K., Yamada, K., Kamiya, M. & Yoshikawa, S.
  • Journal Title: Microbes and Environments Volume: 27 Pages: 513-530
  • Peer Reviewed
• [Journal Article] Two new species of Caloglossa (Delesseriaceae, Ceramiales) from the Americas, C. fluviatilis and C. neotropica sp. nov. (2012)
  • Author(s): Krayesky, D. M., Norris, J., Kamiya, M., West, J. A., Viguerie, M., Wysor, B. S. & Fredericq, S.
  • Journal Title: Phycologia Volume: 51 Pages: 513-530
  • Peer Reviewed
• [Presentation] 緑藻エビヤドリモ属Cladogoniumにおける複子嚢タイプの発見と新目の提唱 (2016)
  • Author(s): 芹澤(松山)和世・今井 正・大貫貴清・中曽雅之・小川 拓・丹羽一夫・神谷充伸・芹澤如比古
  • Organizer: 日本藻類学会2016年大会
  • Place of Presentation: 東京歯科大学（東京）
  • Year and Date: 2016-03-19
• [Presentation] 無性型スジアオノリ（Ulva Prolifera)の遺伝子型間における淡水耐性の違い (2016)
  • Author(s): 小川拓・神谷充伸
  • Organizer: 日本藻類学会2016年大会
  • Place of Presentation: 東京歯科大学（東京）
  • Year and Date: 2016-03-19
• [Presentation] 淡水エビに寄生するエビヤドリモ属Cladogonium藻類には複数種いるのか？ (2015)
  • Author(s): 芹澤如比古・今井 正・大貫貴清・中曽雅之・小川拓・丹羽一夫・神谷充伸・芹澤（松山）和世
  • Organizer: 日本陸水学会甲信越支部会
  • Place of Presentation: 月岡ニューホテル冠月（新潟）
  • Year and Date: 2015-11-28
• [Presentation] 汽水域に生育するスジアオノリの遺伝的・生殖的多様性と分布特性. (2015)
  • Author(s): 小川拓・吉川伸哉・神谷充伸
  • Organizer: 北陸植物学会2015年度大会
  • Place of Presentation: 金沢大学（石川）
  • Year and Date: 2015-06-21
• [Presentation] パルマ藻Triparma laevis NIES-2565のプロトプラストを用いた珪酸質殻構築過程の解析 (2015)
  • Author(s): 山田和正・桂大貴・佐藤晋也・神谷充伸・一宮睦雄・桑田晃・長里千香子・本村泰三・大城香・吉川伸哉
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学箱崎キャンパス（福岡）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Cyanothece sp. Viet Nam 01株とCyanothece PCC7822の細胞外多糖の生産能と化学的性 (2015)
  • Author(s): 牧しづか・兼崎友・佐藤晋也・神谷充伸・大城香・岡島麻衣子・金子達雄・吉川伸哉
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学箱崎キャンパス（福岡）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 日本産褐藻クロモ属（ナガマツモ科）の一新種について (2015)
  • Author(s): 三好浩平・羽生田岳昭・金聖浩・神谷充伸・藤田大介・菊池則雄・伊藤知子・川井浩史
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学箱崎キャンパス（福岡）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] 近縁な海産・淡水産シオグサの遺伝的多様性と分布パターンの比較 (2015)
  • Author(s): 丹羽一夫・吉川伸哉・神谷充伸
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学箱崎キャンパス（福岡）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] パルマ藻の細胞壁の再構築過程 (2014)
  • Author(s): 山田和正・桂大貴・佐藤晋也・神谷充伸・一宮睦雄・桑田晃・長里千香子・本村泰三・大城香・吉川伸哉
  • Organizer: 分子珪藻研究会第一回大会
  • Place of Presentation: 関西学院大学梅田キャンパス（大阪）
  • Year and Date: 2014-12-15
• [Presentation] 単細胞藍藻Cyanothece sp. Viet Nam 01株の細胞外多糖生産における塩濃度の影響と近縁種との比較. (2014)
  • Author(s): 牧しづか・神谷充伸・大城香・岡島麻衣子・金子達雄・吉川伸哉
  • Organizer: ユーグレナ研究会
  • Place of Presentation: 東大寺総合文化センター（奈良）
  • Year and Date: 2014-11-08
• [Presentation] Cell wall formation and growth of Triparma laevis (Parmales, Heterokontophyta) under silicon-limitation. (2014)
  • Author(s): Yamada, K., Yoshikawa, S., Ichinomiya, M., Kuwata, A., Kamiya, M. & Ohki, K.
  • Organizer: The 7th Asian Pacific Phycological Forum
  • Place of Presentation: Wuhan, China
  • Year and Date: 2014-09-20 – 2014-09-24
• [Presentation] Difference of spatial dominance, growth rate, and biomechanical properties between two isomorphic reproductive phases of Chondrus ocellatus (Gigartinales, Rhodophyta). (2014)
  • Author(s): Inoue, N., Suzuki, C., Yoshikawa, S. & Kamiya, M.
  • Organizer: The 7th Asian Pacific Phycological Forum
  • Place of Presentation: Wuhan, China
  • Year and Date: 2014-09-20 – 2014-09-24
• [Presentation] DNAバーコーディング法によるバフンウニの胃内容物解析. (2014)
  • Author(s): 仲野大地・神谷充伸・富永修.
  • Organizer: 日本水産学会2014年度大会
  • Place of Presentation: 九州大学箱崎キャンパス（福岡）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] Extremely high heterozygosities of asexual Ulva prolifera. (2014)
  • Author(s): Ogawa, T., Ohki, K. & Kamiya, M.
  • Organizer: Wando International Marine Algal Symposium
  • Place of Presentation: Wando, Korea
  • Year and Date: 2014-04-16 – 2014-04-19
  • Invited
• [Presentation] A specific antifouling strategy of Sargassum siliquastrum against epiphytism of Neosiphonia harveyi (Rhodophyta). (2014)
  • Author(s): Nakajima, N., Sugimoto, N., Yoshikawa, S., Ohki, K. & Kamiya, M.
  • Organizer: 10th International Phycologidal Congress
  • Place of Presentation: Orlando, Florida, USA
• [Presentation] The geographic scale of speciation in the Indo-Pacific marine red algal genus Portieria. (2014)
  • Author(s): Leliaert, F., D’hondt, S., Payo, D. A., Coppejans, E., De Clerck, O., Verbruggen, H., Gurgel, C. F. D., Spokes, T., Bolton, J. J., Anderson, R. J., Mattio, L., Sherwood, A. R., Johnston, E. T., Schils, T. Kamiya, M., Saunders, G. W., Draisma, S. G. A., Phang, S-M., Lin, S-M., Huisman, J., Gall, L. L. & Zubia, M.
  • Organizer: 10th International Phycologidal Congress
  • Place of Presentation: Orlando, Florida, USA
• [Presentation] パルマ藻から見た珪藻の細胞外被形成機構 (2014)
  • Author(s): 山田和正・大城香・神谷充伸・吉川伸哉
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学
  • Invited
• [Presentation] 新たに分離されたパルマ藻3種1型の性状 (2014)
  • Author(s): 一宮睦雄・山田和正・吉川伸哉・神谷充伸・大城香・原口浩一・桑田晃
  • Organizer: 本プランクトン学会
  • Place of Presentation: 東北大学
• [Presentation] Algal Adventures in New Ireland, Papua New Guinea. (2014)
  • Author(s): West, J., Zuccarello, J., Kamiya, M. & Mineur, F.
  • Organizer: Australasian Society for Phycology and Aquatic Botany Conference
  • Place of Presentation: Sydney, Australia
• [Presentation] 山梨県内のタニシの殻上に着生するシオグサ目藻類 (2014)
  • Author(s): 芹澤如比古・渋谷里夏・早川雄一郎・神谷充伸・芹澤（松山）和世
  • Organizer: 日本藻類学会
  • Place of Presentation: 東邦大学
• [Presentation] パルマ藻Triparma laevisの細胞内微細構造と核分裂様式 (2014)
  • Author(s): 山田和正・長里千香子・本村泰三・一宮睦雄・桑田晃・神谷充伸・大城香・吉川伸哉
  • Organizer: 日本藻類学会
  • Place of Presentation: 東邦大学
• [Presentation] 紅藻ツノマタ類における世代比の季節的変動と世代間における物理・生理特性の比較 (2014)
  • Author(s): 井上直人・鈴木千賀・吉川伸哉・神谷充伸
  • Organizer: 日本藻類学会
  • Place of Presentation: 東邦大学
• [Presentation] 新たに分離されたパルマ藻3株の性状 (2014)
  • Author(s): 一宮睦雄・山田和正・吉川伸哉・神谷充伸・大城香・原口浩一・桑田晃
  • Organizer: 日本海洋学会
  • Place of Presentation: 東京海洋大学
• [Presentation] A unique antifouling mechanism of Sargassum siliquastrum (Phaeophyceae) against epiphytic Neosiphonia harveyi (Rhodophyta). (2014)
  • Author(s): Nakajima, N., Sugimoto, N., Yoshikawa, S., Ohki, K. & Kamiya, M.
  • Organizer: Ectocarpus2013 International Meeting
  • Place of Presentation: 神戸大学
• [Presentation] 紅藻キブリイトグサに対する褐藻ヨレモクの着生阻害機構 (2013)
  • Author(s): 中嶋 登・杉本 直樹・吉川 伸哉・大城 香・神谷 充伸
  • Organizer: 日本藻類学会
  • Place of Presentation: 山梨
• [Presentation] パルマ藻(Triparma laevis)の増殖および殻形成と珪酸塩濃度の関係 (2012)
  • Author(s): 山田和正・吉川伸哉・一宮睦雄・神谷充伸・桑田晃・大城香
  • Organizer: 北陸植物学会
  • Place of Presentation: 富山
• [Presentation] 着生藻に対する褐藻ホンダワラ類の物理的・化学的阻害戦略の検証 (2012)
  • Author(s): 中嶋登・杉本直樹・吉川伸哉・大城香・神谷充伸
  • Organizer: 北陸植物学会
  • Place of Presentation: 富山
• [Presentation] Effects of phosphate, nitrate and silicate on the growth of Triparma laevis (Parmales, Heterokonta) cultured in synthetic medium (2012)
  • Author(s): Yamada, K., Yoshikawa, S., Ichinomiya, M., Sasase, M., Kuwata, A., Kamiya, M. & Ohki, K.
  • Organizer: Association for the Sciences of Limnology and Oceanography
  • Place of Presentation: Lake Biwa, Otsu, Shiga, Japan
• [Presentation] ホンダワラ類15種における褐藻フロロタンニンの含有量と成分組成の比較 (2012)
  • Author(s): 中嶋登・杉本直樹・吉川伸哉・大城香・神谷充伸
  • Organizer: 日本藻類学会
  • Place of Presentation: 北海道
• [Presentation] Exopolysaccharide of freshwater unicellular cyanobacterium Cyanothece sp. Vietnam 01; synthesis and physicochemical characterization. (2012)
  • Author(s): Ohki, K., Quyen, N. T. L., Yoshikawa, S., Kamiya, M., Okajima, M., Kaneko, T. & Thi, T. H.
  • Organizer: 14th International Symposium on Phototrophic Prokaryotes
  • Place of Presentation: Porto, Portugal
• [Presentation] 低珪酸塩条件下におけるパルマ藻(Triparma laevis)の殻形成と増殖 (2012)
  • Author(s): 山田和正・吉川伸哉・一宮睦雄・神谷充伸・桑田晃・大城香
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫
• [Presentation] ラン藻Cyanobium sp. Suigetsu CG2による細胞外多糖生産とその性状 (2012)
  • Author(s): 大城香・芥川遼輔・吉川伸哉・神谷充伸・Quyen, N. T. L.・金子達雄・岡島麻衣子・金崎友
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫
• [Presentation] Patterns of geographic distribution and sexual vs asexual reproduction in Caloglossa vieillardii in Australia (2012)
  • Author(s): Kamiya, M., West, J. & Zuccarello, G. C.
  • Organizer: Australasian Society for Phycology and Aquatic Botany Conference
  • Place of Presentation: Adelaide, Australia
• [Presentation] Isolation and characterization of Parmales from the Oyashio region, western North Pacific. (2012)
  • Author(s): Ichinomiya, M., Yoshikawa, S., Kamiya, M., Ohki, K., Takaichi, S. & Kuwata, A.
  • Organizer: First Asian Marine Biology Symposium
  • Place of Presentation: Thailand
• [Presentation] 海藻と植物プランクトンの利用
  • Author(s): 神谷充伸
  • Organizer: バイオテクノロジー研究会
  • Place of Presentation: 福井大学
  • Invited
• [Presentation] 紅藻ササバアヤギヌ（Caloglossa vieillardii）におけるアポミクトの起源
  • Author(s): 佐波恵理加・John A. West・吉川伸哉・神谷充伸
  • Organizer: 北陸植物学会
  • Place of Presentation: 金沢大学
• [Presentation] 多様な塩濃度環境に生育するシオグサ属藻類における集団遺伝学的解析
  • Author(s): 丹羽一夫・早川 雄一郎・吉川伸哉・神谷充伸
  • Organizer: 北陸植物学会
  • Place of Presentation: 金沢大学
• [Book] 海藻（ネイチャーウォッチングガイドブック） (2012)
  • Author(s): 監修：神谷充伸、おしば：野田三千代、写真：阿部秀樹
  • Total Pages: 271
  • Publisher: 誠文堂新光社
• [Book] 藻類ハンドブック (2012)
  • Author(s): 監修：渡辺 信、編集・分筆：神谷充伸
  • Total Pages: 768
  • Publisher: NTS