Diversity and evolution of storage glucan synthetic systems in photosynthetic organisms

• Project/Area Number: 24570114
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Fujiwara Shoko 東京薬科大学, 生命科学部, 准教授 (30266895)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 貯蔵多糖 / 光合成産物 / デンプン / グリコーゲン / セミアミロペクチン / 光合成生物 / 紅藻 / 緑藻

Outline of Final Research Achievements

Photosynthetic organisms have a wide variety of storage glucans. To clarify the evolutionary processes that led to syntheses of starch and β-glucan, we performed the following analyses: 1. Activities and expression patterns of glucan synthesis-related enzymes were compared between the primitive rhodophytes Cyanidioschyzon (semi-amylopectin-type) and Cyanidium (glycogen-type). To examine roles of the enzymes isoamylase and α-glucan brancing enzyme, disruptants of the genes were constructed in Cyanidioschyzon and the structures of the glucan were characterized. 2. The nucleotide sequence of KRE6 homologue obtained from the haptophyte Pleurochrysis cDNA library has amino acid residues conserved in β-glucan binding proteins. Knockdown of this gene was examined to identify a function of the gene product.

Research Products

• [Journal Article] Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria. (2016)
  • Author(s): Sakurai, T., Aoki, M., Ju, X., Ueda, T., Nakamura, Y., Fujiwara, S., Umemura, T., Tsuzuki, M., and Minoda, A.
  • Journal Title: Bioresource Technology Volume: 200 Pages: 861-866
  • DOI: 10.1016/j.biortech.2015.11.014
  • Peer Reviewed / Open Access
• [Journal Article] Pleurochrysome: A web-database of Pleurochrysis transcripts and orthologues among heterogeneous algae. (2016)
  • Author(s): Yamamoto N, Kudo T, Fujiwara S, Takatsuka Y, Hirokawa Y, Tsuzuki M, Takano T, Kobayashi M, Suda K, Asamizu E, Yokoyama K, Shibata D, Tabata S, Yano K
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 1 Pages: e6-e6
  • DOI: 10.1093/pcp/pcv195
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Arsenic metabolism in cyanobacteria. (2016)
  • Author(s): Miyashita, S., C. Murota, K. Kondo, S. Fujiwara and M. Tsuzuki
  • Journal Title: Environ. Chem. Volume: -
  • Peer Reviewed
• [Journal Article] Genes for a series of proteins that are involved in glucose catabolism are upregulated by the Hik8-cascade in Synechocystis sp. PCC 6803. (2015)
  • Author(s): Okada, K., E. Horii, Y. Nagashima, M. Mitsui, H. Matsuura, S. Fujiwara, M. Tsuzuki
  • Journal Title: Planta Volume: Published online: 03 March Issue: 6 Pages: 1-10
  • DOI: 10.1007/s00425-015-2270-z
  • Peer Reviewed
• [Journal Article] Diversity of reaction characteristics of glucan branching enzymes and the fine structure of α-glucan from various sources. (2014)
  • Author(s): Sawada, T., Y. Nakamura, T. Ohdan, A. Saitoh, Francisco PB Jr, E. Suzuki, N. Fujita, T. Shimonaga, S. Fujiwara, M. Tsuzuki, C. Colleoni, S. Ball
  • Journal Title: Arch. Biochem. Biophys. Volume: 562 Pages: 9-21
  • DOI: 10.1016/j.abb.2014.07.032
  • Peer Reviewed
• [Journal Article] Unprecedented cell-selection using ultra-quick freezing combined with aquaporin expression. (2014)
  • Author(s): Kato, Y., T. Miyauchi, Y. Abe, D. Koji´c, M. Tanaka, N. Chikazawa, Y. Nakatake, S. B. H. Ko, D. Kobayashi, A. Hazama, S. Fujiwara, T. Uchida, M. Yasui
  • Journal Title: PLoS ONE Volume: 9 Issue: 2 Pages: e87644-e87644
  • DOI: 10.1371/journal.pone.0087644
  • Peer Reviewed / Open Access
• [Journal Article] Localization and associative strength of acid polysaccharides in coccoliths of Pleurochrysis haptonemofera (Haptophyta) predicted from their extractability from partiallydecalcified coccoliths (2013)
  • Author(s): Y. Hirokawa, S. Matsuzuka, S. Itayama, T. Uchida, S. Fujiwara, N. Ozaki, H. Nagasawa and M. Tsuzuki
  • Journal Title: Open J. Mar. Sci Volume: 3 Issue: 01 Pages: 48-54
  • DOI: 10.4236/ojms.2013.31005
  • Peer Reviewed
• [Journal Article] Arsenic tolerance in a Chlamydomonas photosynthetic mutant is due to reduced arsenic uptake even in light conditions. (2012)
  • Author(s): Murota, C., H. Matsumoto, S. Fujiwara, Y. Hiruta, S. Miyashita, M. Shimoya, I. Kobayashi, M. O. Hudock, R. K. Togasaki, N. Sato and M. Tsuzuki
  • Journal Title: Planta Volume: 236 Issue: 5 Pages: 1395-403
  • DOI: 10.1007/s00425-012-1689-8
  • Peer Reviewed
• [Journal Article] Cyanobacteria produce arsenosugars. (2012)
  • Author(s): Miyashita, S., S. Fujiwara, M. Tsuzuki and T. Kaise
  • Journal Title: Environ. Chem. Volume: 9 Issue: 5 Pages: 474-84
  • DOI: 10.1071/en12061
  • Peer Reviewed
• [Journal Article] Two regulatory networks mediated by light and glucose involved in glycolytic gene expression in cyanobacteria. (2012)
  • Author(s): Tabei, Y., Okada, K., Horii, E., Mitsui, M., Nagashima, Y., Sakai, T., Yoshida, T., Kamiya, A., Fujiwara, S., Tsuzuki, M..
  • Journal Title: Plant Cell Physiology Volume: 53 Issue: 10 Pages: 1720-1727
  • DOI: 10.1093/pcp/pcs115
  • NAID: 10031122174
  • Peer Reviewed
• [Presentation] Synechocystis sp. PCC 6803のリン酸輸送体変異株を用いたヒ素耐性機構の解明. (2016)
  • Author(s): 室田知里、辻下真紀、畑山遥、山下貴矢、松本寛子、西弘貴、佐藤典裕、藤原祥子、都筑幹夫
  • Organizer: 日本藻類学会第40回大会
  • Place of Presentation: 東京
  • Year and Date: 2016-03-18
• [Presentation] 円石藻 Pleurochrysis の円石形成 ―ベースプレートの性質と関連遺伝子の探索―. (2015)
  • Author(s): 櫻田舜人、浅川航輝、遠藤博寿、鈴木道生、小暮敏博、藤原祥子、都筑幹夫
  • Organizer: 第10回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2015-12-06
• [Presentation] ゼブラフィッシュ初期胚の耳石形成における炭酸脱水酵素の関与. (2015)
  • Author(s): 松本寛子、藤原祥子、都筑幹夫
  • Organizer: 第10回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2015-12-06
• [Presentation] Pleurochrysisの円石形成（in vitro石灰化）. (2015)
  • Author(s): 櫻田舜人、鈴木道生、小暮敏博、板山翔、都筑幹夫、藤原祥子
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-06
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803 のリン, ヒ素取り込み. (2015)
  • Author(s): 室田知里、辻下真貴、藤原祥子、都筑幹夫
  • Organizer: 日本藻類学会第39回大会
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2015-03-20 – 2015-03-25
• [Presentation] 円石藻Pleurochrysisの円石におけるベースプレートの性質. (2014)
  • Author(s): 櫻田舜人、鈴木道生、小暮敏博、板山翔、松塚悟、小林貴恵、都筑幹夫、藤原祥子
  • Organizer: 東京大学大気海洋研究所共同利用研究集会「バイオミネラリゼーションと石灰化 ―遺伝子から地球環境まで―」＆第9回バイオミネラリゼーションワークショップ共催シンポジウム
  • Place of Presentation: 東京大学大気海洋研究所講堂
  • Year and Date: 2014-12-12 – 2014-12-13
• [Presentation] シアノバクテリアとクラミトモナスにおけるヒ素・リン酸の取り込みと代謝. (2014)
  • Author(s): 辻下真貴、室田知里、山下貴矢、松本寛子、西弘貴、佐藤典裕、藤原祥子、都筑幹夫
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-13
• [Presentation] クラミドモナスの葉緑体形態に及ぼす阻害剤の影響 (2014)
  • Author(s): 4. 植田達也、櫻井俊宏、藤原祥子、都筑幹夫
  • Organizer: 日本藻類学会第38回大会
  • Place of Presentation: 船橋
• [Presentation] 円石藻Pleurochrysis haptonemoferaの石灰化に関する研究：円石構成成分の機能解析と石灰化関連遺伝子の探索 (2014)
  • Author(s): 5. 板山翔、小林貴恵、藤原祥子、遠藤博寿、長澤寛道、都筑幹夫
  • Organizer: 日本藻類学会第38回大会
  • Place of Presentation: 船橋
• [Presentation] 気相及び液相中におけるクロレラの光合成と増殖特性 －バイオマス生産の生物的基礎として－ (2013)
  • Author(s): 白武拓磨、磯田春奈、戸谷藍子、出雲旦子、小高徹郎、西條広隆、藤原祥子、都筑幹夫
  • Organizer: 第15回マリンバイオテクノロジー学会大会
  • Place of Presentation: 沖縄
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803のポリリン酸キナーゼ遺伝子破壊株（Δppk1）の解析 (2013)
  • Author(s): 西弘貴、佐藤典裕、藤原祥子、都筑幹夫
  • Organizer: 第15回マリンバイオテクノロジー学会大会
  • Place of Presentation: 沖縄
• [Presentation] Localization and associative strengths of acidic polysaccharides of Pleurochrysis haptonemofera. (2012)
  • Author(s): 1. Itayama, S., S. Matsuzuka, Y. Hirokawa, S. Fujiwara, K. Nakanishi, and M. Tauzuki
  • Organizer: The 9th Asia-Pacific Marine Biotechnology Conference
  • Place of Presentation: Kochi
• [Presentation] シアノバクテリアSynechocystis sp. PCC 6803のヒ素耐性に及ぼすポリリン酸代謝の影響
  • Author(s): 西弘貴、辻下真貴、佐藤典裕、藤原祥子、都筑幹夫
  • Organizer: ユーグレナ研究会第29回研究集会
  • Place of Presentation: つくば
• [Book] 藻類ハンドブックの一部 (2012)
  • Author(s): 渡辺信監修
  • Total Pages: 4
  • Publisher: エヌティーエス
• [Remarks] Pleurochrysome
  • URL: http://bioinf.mind.meiji.ac.jp/phapt/index.html