| Project/Area Number |
24570116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Japan Women's University |
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Principal Investigator |
IMAICHI Ryoko 日本女子大学, 理学部, 教授 (60112752)
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| Co-Investigator(Kenkyū-buntansha) |
ATSUSHI Ebihara 独立行政法人国立科学博物館, 植物研究部, 研究員 (20435738)
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| Co-Investigator(Renkei-kenkyūsha) |
YUKI Tsujita 佐賀大学, 農学部, 准教授 (80522523)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 配偶体 / シダ植物 / 進化 / 共生 / 菌根菌 / アーバスキュラー菌 / ハビタット / 形態 / AM菌 / グロムス門 / 着生 |
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| Outline of Final Research Achievements |
The aim of this research is to verify hypotheses that the heart shaped, terrestrial gametophytes of ferns contain AM mycorrhizal fungi in its midrib called the cushion, and that there is a significant relation between the cushion thickness and the AM infection rate. As the first step of this research, we analyzed here basal taxa of ferns (Angiopteridaceae, Osmundaceae, Gleicheniaceae, Plagiogyriaceae, and Cyatheaceae). We collected wild gametophytes in many regions of Japan. Gametophytes collected were identified at species rank using rbcL genes, and cut longitudinally by the resin-sectioning method to check presence or absence of AM fungi, and cushion thickness. In total, 274 individuals of six genera and five families, were analyzed. The AM fungi infection rate was relatively high (58 -95%), and cushion thickness was relatively thick (207-470 micrometers).Molecular identification using SSU sequences showed that the most commonly found AM fungi were Glomeraceae.
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