Sperm recognition domain in mammalian egg coat matrix
| Project/Area Number |
24570125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Chiba University |
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Principal Investigator |
YONEZAWA Naoto 千葉大学, 理学(系)研究科(研究院), 准教授 (80212314)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGIDA Mitsuaki 順天堂大学, 医学研究所, 准教授 (80365569)
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| Co-Investigator(Renkei-kenkyūsha) |
TANOKURA Masaru 東京大学, 農学生命科学研究科, 教授 (60136786)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 糖タンパク質 / 受精 / 透明帯 / 小角X線散乱 / 細胞外マトリクス |
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| Outline of Final Research Achievements |
Mammalian egg coat recognizes sperm in a species selective manner on fertilization and blocks polyspermy after establishment of fertilization. Molecular mechanisms on these phenomena are to be clarified. We found that porcine sperm prefer beta-galactose and bovine sperm prefer alpha-mannose by using glycolipid analogues. Bovine egg coat is composed of three proteins called ZP2, ZP3 and ZP4. We found that an N-glycan linked to the middle region of ZP3 is involved in sperm recognition. On the other hand, we have previously found that in porcine egg coat an N-glycan linked to the middle region of ZP4 is involved in sperm recognition. Thus, the roles of ZP3 and ZP4 in sperm recognition might be different between cattle and pig.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Coordination structures of Mg^<2+> and Ca^<2+> in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain coo- groups2013
Author(s)
N. Suzuki, L. F. Imai, Y. Kato, K. Nagata, Y. Ohashi, K. Kuchitsu, M. Tanokura, A. Sakamoto, M. Nara, M. Nakano, and N. Yonezawa
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Journal Title
Biopolymers
Volume: 99
Issue: 7
Pages: 472-483
DOI
Related Report
Peer Reviewed
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[Journal Article] Binding of Sperm to the Zona Pellucida Mediated by Sperm Carbohydrate-Binding Proteins is not Species-Specific in Vitro between Pigs and Cattle.2013
Author(s)
Takahashi K, Kikuchi K, Uchida Y, Kanai-Kitayama S, Suzuki R, Sato R, Toma K, Geshi M, Akagi S, Nakano M, Yonezawa N
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Journal Title
Biomolecules
Volume: 3(1)
Issue: 1
Pages: 85-107
DOI
Related Report
Peer Reviewed
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[Presentation] Coordination structures of Mg(2+) and Ca(2+) in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain COO- groups
Author(s)
Nanao Suzuki, Lica Fabiana Imai, Yusuke Kato, Koji Nagata, Yuko Ohashi, Kazuyuki Kuchitsu, Masaru Tanokura, Akira Sakamoto, Masayuki Nara, Minoru Nakano, Naoto Yonezawa
Organizer
7th International Conference on Advanced Vibrational Spectroscopy
Place of Presentation
神戸国際会議場
Related Report
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