Sperm recognition domain in mammalian egg coat matrix

• Project/Area Number: 24570125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Chiba University
• Principal Investigator: YONEZAWA Naoto 千葉大学, 理学(系)研究科(研究院), 准教授 (80212314)
• Co-Investigator(Kenkyū-buntansha): YANAGIDA Mitsuaki 順天堂大学, 医学研究所, 准教授 (80365569)
• Co-Investigator(Renkei-kenkyūsha): TANOKURA Masaru 東京大学, 農学生命科学研究科, 教授 (60136786)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 糖タンパク質 / 受精 / 透明帯 / 小角X線散乱 / 細胞外マトリクス

Outline of Final Research Achievements

Mammalian egg coat recognizes sperm in a species selective manner on fertilization and blocks polyspermy after establishment of fertilization. Molecular mechanisms on these phenomena are to be clarified. We found that porcine sperm prefer beta-galactose and bovine sperm prefer alpha-mannose by using glycolipid analogues. Bovine egg coat is composed of three proteins called ZP2, ZP3 and ZP4. We found that an N-glycan linked to the middle region of ZP3 is involved in sperm recognition. On the other hand, we have previously found that in porcine egg coat an N-glycan linked to the middle region of ZP4 is involved in sperm recognition. Thus, the roles of ZP3 and ZP4 in sperm recognition might be different between cattle and pig.

Research Products

• [Journal Article] Posttranslational modifications of zona pellucida proteins (2014)
  • Author(s): Naoto Yonezawa
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 759 Pages: 111-140
  • DOI: 10.1007/978-1-4939-0817-2_6
  • ISBN: 9781493908165, 9781493908172
  • Acknowledgement Compliant
• [Journal Article] Calcium-dependent structural changes in human reticulocalbin-1 (2014)
  • Author(s): Nanao Suzuki, Shoko Ban, Eriko Itoh, Sisi Chen, Lica Fabiana Imai, Yoriko Sawano, Takuya Miyakawa, Masaru Tanokura, Naoto Yonezawa
  • Journal Title: Journal of Biochemistry Volume: in press Issue: 5 Pages: 281-293
  • DOI: 10.1093/jb/mvu003
  • Peer Reviewed
• [Journal Article] Coordination structures of Mg^<2+> and Ca^<2+> in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain coo- groups (2013)
  • Author(s): N. Suzuki, L. F. Imai, Y. Kato, K. Nagata, Y. Ohashi, K. Kuchitsu, M. Tanokura, A. Sakamoto, M. Nara, M. Nakano, and N. Yonezawa
  • Journal Title: Biopolymers Volume: 99 Issue: 7 Pages: 472-483
  • DOI: 10.1002/bip.22203
  • Peer Reviewed
• [Journal Article] Binding of Sperm to the Zona Pellucida Mediated by Sperm Carbohydrate-Binding Proteins is not Species-Specific in Vitro between Pigs and Cattle. (2013)
  • Author(s): Takahashi K, Kikuchi K, Uchida Y, Kanai-Kitayama S, Suzuki R, Sato R, Toma K, Geshi M, Akagi S, Nakano M, Yonezawa N
  • Journal Title: Biomolecules Volume: 3(1) Issue: 1 Pages: 85-107
  • DOI: 10.3390/biom3010085
  • Peer Reviewed
• [Journal Article] Estimation of the detection rate in STR analysis by determining the DNA degradation ratio using quantitative PCR. (2013)
  • Author(s): Kitayama T, Fujii K, Nakahara H, Mizuno N, Kasai K, Yonezawa N, Sekiguchi K
  • Journal Title: Leg Med (Tokyo) Volume: 15(1) Issue: 1 Pages: 1-6
  • DOI: 10.1016/j.legalmed.2012.07.003
  • Peer Reviewed
• [Presentation] ウシ透明帯糖タンパク質ZP3/ZP4複合体の相互作用部位 (2014)
  • Author(s): 田中宏明、上田 美冬、田中 佑里恵、建部 七海、劉 朔、鈴木 七緒、米澤 直人
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: 横浜産貿易ホール マリネリア
  • Year and Date: 2014-06-26
• [Presentation] ヒトReticulocalbin-1のカルシウム依存的構造変化 (2014)
  • Author(s): 鈴木 七緒、陳 思思、今井 ファビアナ リカ、澤野 頼子、宮川 拓也、田之倉 優、米澤 直人
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: 横浜産貿易ホール マリネリア
  • Year and Date: 2014-06-26
• [Presentation] ウシ卵子透明帯糖タンパク質ZP3/ZP4複合体の精子結合部位 (2013)
  • Author(s): 上田美冬，建部七海，田中佑里恵，鈴木香緒理，帯川侑也，鈴木七緒，米澤直人
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Heterologous zona pellucida binding and oocyte penetration by sperm in pig and bovine (2012)
  • Author(s): Naoto Yonezawa, Yasuomi Uchida, Kazuya Takahashi, Reiichiro Suzuki, SaekoKanai-Kitayama, Reiko Sato, Kazunori Toma, Kazuhiro Kikuchi and Minoru Nakano
  • Organizer: The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: Hotel Nagoya Garden Palace, Nagoya, Japan
• [Presentation] ウシ卵子透明帯N結合型糖鎖の精子結合への関与 (2012)
  • Author(s): 鈴木香緒理、帯川侑也、鈴木七緒、米澤直人
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] ウシ卵子透明帯O結合型糖鎖は精子結合には不必要である (2012)
  • Author(s): 帯川侑也、鈴木香緒理、鈴木七緒、米澤直人
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] ヒトReticulocalbin-1のカルシウム依存的構造変化 (2012)
  • Author(s): 鈴木七緒、陳思思、坂象子、今井ファビアナリカ、永田宏次、田之倉優、中野實、米澤直人
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] XANESを用いたヒトCOMMDタンパク質における銅周囲の局所構造解析 (2012)
  • Author(s): 高橋慧、金子拓真、三木達郎、滝博行、宮川慶大、丸尾啓仁、小原菜摘、小西健久、藤川高志、米澤直人、酒巻真粧子、野村昌治
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] ヒトReticulocalbin-1のカルシウム依存的構造変化
  • Author(s): 鈴木七緒，陳思思，宮川拓也，永田宏次，田之倉優，中野實，米澤直人
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Coordination structures of Mg(2+) and Ca(2+) in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain COO- groups
  • Author(s): Nanao Suzuki, Lica Fabiana Imai, Yusuke Kato, Koji Nagata, Yuko Ohashi, Kazuyuki Kuchitsu, Masaru Tanokura, Akira Sakamoto, Masayuki Nara, Minoru Nakano, Naoto Yonezawa
  • Organizer: 7th International Conference on Advanced Vibrational Spectroscopy
  • Place of Presentation: 神戸国際会議場
• [Presentation] Calcium-dependent structural changes in human reticulocalbin-1
  • Author(s): Nanao Suzuki, Syoko Ban, Eriko Itoh, Sisi Chen, Fabiana L. Imai, Yoriko Sawano, Masaru Tanokura, Naoto Yonezawa
  • Organizer: The 27th Annual Symposium of the Protein Society
  • Place of Presentation: Boston Marriott Copley Place, USA
• [Book] Sexual Reproduction in Animals and Plants のうちChapter 34 Involvement of Carbohydrate Residues of the Zona Pellucida in In Vitro Sperm Recognition in Pigs and Cattleを担当 (2014)
  • Author(s): Naoto Yonezawa 分担執筆 (edited by Hitoshi Sawada, Naokazu Inoue and Megumi Iwano)
  • Publisher: SpringerOpen