Structural basis for the post translational membrane insertion by GET complex
Project/Area Number |
24570126
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 結晶構造解析 / テイルアンカー型タンパク質 / 膜タンパク質 / 構造生物学 / X線結晶解析 / TA タンパク質 / X線結晶構造解析 |
Outline of Final Research Achievements |
Get3 is the specific targeting factor for the tail anchored proteins, which is defined by the C-terminal single transmembrane domain. After capturing the TA proteins, Get3 is further localized on the ER membrane through the interaction with Get1/Get2 membrane proteins. In this study, we succeeded to solve the crystal structure of the complex between Get3 and Get1 cytosolic domain. In addition, we succeeded to purify the Get3-TA complex coexpressed in E. coli. By using the mutant Get3, we succeeded to obtain the Get3-TA complex at a molar ratio of 2:1 (Get3 dimer bound to single TA). Further, we succeeded to purify Get1, Get2 using insect cell expression system.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Structures of CYLD USP with Met1- or Lys63-linked diubiquitin reveal mechanisms for dual specificity2015
Author(s)
Sato, Y., Goto, E., Shibata, Y., Kubota, Y., Yamagata, A., Goto-Ito, S., Kubota, K., Inoue, J., Takekawa, M., Tokunaga, F. and Fukai, S.
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Journal Title
Nat. Struct. Mol. Biol.
Volume: 22
Issue: 3
Pages: 222-229
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Switching and emergence of CTL epitopes in HIV-1 infection2014
Author(s)
Han, C., Kawana-Tachikawa, A., Shimizu, A., Zhu D., Nakamura, H., Adachi, E., Kikuchi, T., Koga, M., Koibuchi, T., Gao, G.F., Sato, Y., Yamagata, A., Martin, E., Fukai, S., Brumme, Z.L.,Iwamoto, A.
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Journal Title
Retrovirolgy
Volume: 11
Issue: 1
Pages: 38-38
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Switching and emergence of CTL epitopes in HIV-1 infection2014
Author(s)
Han C, Kawana-Tachikawa A, Shimizu A, Zhu D, Nakamura H, Adachi E, Kikuchi T, Koga M, Koibuchi T, Gao GF, Sato Y, Yamagata A, Martin E, Fukai S, Brumme ZL, Iwamoto A
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Structure of TCR and antigen complexes at an immunodominant CTL epitope in HIV-1 infection2013
Author(s)
Shimizu, A., Kawana-Tachikawa. A., Yamagata, A., Han, C., Zhu, D., Sato, Y., Nakamura, H., Koibuchi, T., Carlson, J., Martin, E., Brumme, C.J., Shi, Y., Gao, G.F., Brumme, Z.L., Fukai, S., Iwamoto, A.
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 3097-3097
DOI
Related Report
Peer Reviewed
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