Study of Dynamic Structures of Protein molecules by single molecule force spectroscopy

• Project/Area Number: 24570128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yamagata University (2014); Japan Advanced Institute of Science and Technology (2012-2013)
• Principal Investigator: KAWAKAMI MASARU 山形大学, 理工学研究科, 准教授 (70452117)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: タンパク質 / 原子間力顕微鏡 / 一分子計測 / ポリペプチド / １分子測定 / 粘弾性 / 天然変性タンパク質

Outline of Final Research Achievements

We Studied the dynamic structures of various polypeptides at single molecule level using the technique of Atomic force microscopy. Single molecule force spectroscopy reveaed that Polyglutamic acid, polylysine have very flexible structure, while polyproline has a very rigid, resilient structure.

Research Products

• [Journal Article] Optimizing the calculation of energy landscape parameters from single-molecule protein unfolding experiments. (2015)
  • Author(s): KM. Tych, ML. Hughes, J. Bourke, Y. Taniguchi, M. Kawakami, DJ. Brockwell, L. Dougan
  • Journal Title: Physical Review E Volume: 91 Issue: 1 Pages: 012710-012710
  • DOI: 10.1103/physreve.91.012710
  • Peer Reviewed
• [Journal Article] Nano-mechanics of HaloTag Tethers (2013)
  • Author(s): Popa I, Berkovich R, Alegre-Cebollada J, Badilla C, Rivas-Pardo J, Taniguchi Y, Kawakami M and Fernandez J
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 34 Pages: 12762-12771
  • DOI: 10.1021/ja4056382
  • Peer Reviewed
• [Journal Article] Mechanical Unfolding Studies of Protein Molecules (2012)
  • Author(s): Yukinori Taniguchi, Akiko Kobayashi and Masaru Kawakami
  • Journal Title: BIOPHYSICS Volume: 8 Issue: 11 Pages: 51-58
  • DOI: 10.1371/journal.pone.0049003
  • NAID: 130002061597
  • Peer Reviewed
• [Journal Article] Recent advances in single-molecule biophysics with the use of Atomic Force Microscopy (2012)
  • Author(s): Masaru Kawakami and Yukinori Taniguchi
  • Journal Title: Advances in Chemical Physics Volume: 146 Pages: 89-132
• [Presentation] Single molecule force spectroscopy by AFM indicates highly resilient structure of Polyproline helix (2013)
  • Author(s): Masaru Kawakami
  • Organizer: 日本生物物理学会
  • Place of Presentation: 京都国際会館
• [Presentation] Single molecule force spectroscopy by AFM indicates highly resilient structure of Polyproline helix (2013)
  • Author(s): Masaru Kawakami
  • Organizer: IMS Workshop
  • Place of Presentation: 岡崎カンファレンスセンター
• [Book] 生体分子の動きを探る ―揺らぎと生体機能― (2013)
  • Author(s): 川上勝
  • Total Pages: 300
  • Publisher: 化学同人
• [Book] Single Molecule Biophysics: Experiments and Theory (2012)
  • Author(s): T. Komatsuzaki, M. Kawakami, S. Takahashi, H. Yang , R. J. Silbey, S. A. Rice, A. R. Dinner
  • Total Pages: 300
  • Publisher: John Wiley & Sons Inc.，