| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We constructed several amyloid fibril formation systems in vitro to evaluate the roles of the various biological molecules or reaction conditions in the amyloid fibril formation. The key findings of this study are as follows: (1) We developed a near-physiological Alzheimer's disease amyloid β-peptide (Aβ) fibril formation system for evaluating the efficiency of protein components to induce the nucleation of Aβ-peptide. To suppress spontaneous Aβ nucleation, the system includes protein coated Sepharose beads and low concentration of Aβ peptide solution without air in the reaction vessel. Using the system, we indicated several basement membrane components accelerate Aβ fibril formation in vitro. (2) To further decrease the concentration of Aβ peptide in the reaction, we are developing an open flow system, where near physiological concentrations of Aβ peptide are continuously supplied to the reaction surface.
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