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Structural study of florigen and the receptor complex

Research Project

Project/Area Number 24570133
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionKyoto University (2014)
Nara Institute of Science and Technology (2012-2013)

Principal Investigator

OHKI Izuru  京都大学, 工学(系)研究科(研究院), 研究員 (80418574)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsX線結晶解析 / NMR / 植物 / 植物ホルモン / 構造生物学 / 花成 / フロリゲン / 核磁気共鳴
Outline of Final Research Achievements

Florigen is a mobile plant hormone that determines the flowering time in higher plants, but its regulatory mechanism remains unclear. In this study, we focused on an anti-florigen, which suppresses flowering antagonizing with florigen, to clarify the role in floral regulation. From structural and biochemical analysis, anti-florigen was found to form a stable complex with florigen receptor and inhibit formation of florigen-receptor complex. The bindings of florigen and anti-florigen to receptor were exchangeable, suggesting that the balance of the two proteins is important for determining floral transition.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (7 results) (of which Invited: 4 results)

  • [Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015

    • Author(s)
      Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 41 Pages: 28569-78

    • DOI

      10.1074/jbc.m114.603282

    • Description
      (in press)(印刷中)
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac12014

    • Author(s)
      Kosami K
    • Journal Title

      Acta Cryst.

      Volume: F70 Issue: 1 Pages: 113-115

    • DOI

      10.1107/s2053230x13033645

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メチル化リジンの化学シフトと塩橋との相関に関する理論的・実験的研究2014

    • Author(s)
      服部 良一、Jakub Sebera、Vladimir Sychrovsky、古板 恭子、大木  出、池上 貴久、藤原敏道、児嶋 長次郎
    • Journal Title

      日本核磁気共鳴学会機関誌

      Volume: 5 Pages: 56-59

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Structure and function of florigen and the receptor complex2013

    • Author(s)
      田岡 健一郎、大木 出、辻 寛之、児嶋 長次郎、島本 功
    • Journal Title

      Trends Plant Sci

      Volume: 18 Issue: 5 Pages: 287-294

    • DOI

      10.1016/j.tplants.2013.02.002

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Utilization of lysine ¹³C-methylation NMR for protein-protein interaction studies.2013

    • Author(s)
      Hattori Y, Furuita K, Ohki I, Ikegami T, Fukada H, Shirakawa M, Fujiwara T, Kojima C.
    • Journal Title

      J Biomol NMR.

      Volume: 55 Issue: 1 Pages: 19-31

    • DOI

      10.1007/s10858-012-9675-9

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila.2013

    • Author(s)
      Nakano T, Ohki I, Yokota A, Ashida H.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 7 Pages: e67385-e67385

    • DOI

      10.1371/journal.pone.0067385

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 結晶構造を用いたNMRによる相互作用解析とStructural Imagingへの展望2014

    • Author(s)
      大木出
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪府吹田市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] メチル化リジンの化学シフトと塩橋との相関に関する理論的・実験的研究2013

    • Author(s)
      服部良一,Jakub Sebera,Vladimir Sychrovsky,古板恭子,大木出,池上貴久,藤原敏道,児嶋長次郎
    • Organizer
      第52回NMR討論会
    • Place of Presentation
      石川県金沢市
    • Related Report
      2013 Research-status Report
  • [Presentation] 植物花成ホルモン(フロリゲン)受容体による花成制御機構の分子基盤

    • Author(s)
      大木出、古板恭子、林こころ、田岡健一郎、辻寛之、中川敦史、島本功、児嶋長次郎
    • Organizer
      第85回日本生化学会年会
    • Place of Presentation
      福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] リジンの13Cメチル化標識法を使ったNMRによる蛋白質間相互作用解析の有効性

    • Author(s)
      大木出、服部良一、古板恭子、池上貴久、深田はるみ、白川昌宏、藤原敏道、児嶋長次郎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 花成ホルモン・フロリゲンの受容と機能のメカニズム

    • Author(s)
      辻寛之、田岡健一郎、大木出、玉置祥二郎、中島千佳、中村洋之、稲田のりこ、児嶋長次郎、島本功
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] イネ低分子量G蛋白質OsRac1の結晶構造解析と変異体デザインによるNADPHオキシダーゼOsRbohB制御機構の解明

    • Author(s)
      小佐見謙一、大木出、長野稔、林こころ、田畑亮、薄衣砂弥香、川崎努、中川敦史、藤原敏道、島本功、児嶋長次郎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] フロリゲンによる花成の分子機構と花成以外の形態形成制御

    • Author(s)
      島本功、田岡健一郎、辻寛之、大木出
    • Organizer
      第85回日本生化学会年会
    • Place of Presentation
      福岡国際会議場、マリンメッセ福岡
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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