| Project/Area Number |
24570137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
IHARA YOSHITO 和歌山県立医科大学, 医学部, 教授 (70263241)
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| Co-Investigator(Kenkyū-buntansha) |
INAI Yoko 和歌山県立医科大学, 医学部, 助教 (20316087)
IKEZAKI Midori 和歌山県立医科大学, 医学部, 助教 (40549747)
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| Co-Investigator(Renkei-kenkyūsha) |
MANABE Shino 独立行政法人理化学研究所, 基幹研究所, 専任研究員 (60300901)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 糖質 / 糖タンパク質 / マンノース / 細胞増殖 / TGF-β / トロンボスポンジン / 糖ペプチド / シグナル伝達 |
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| Outline of Final Research Achievements |
In cultured fibroblast NRK49F cells, TGF-β-induced cell proliferation was suppressed by C-mannosyl (C-Man) peptides through the downregulation of TGF-β/Smad signaling pathway. In the cells, target proteins bound to the C-Man peptides were purified by using synthesized C-Man-WSPWC-biotin and avidin-conjugated beads, then characterized by MS finger printing analysis. The target proteins were identified as Hsc70 and myosin 1c. We found that Hsc70 is involved in the regulation of TGF-β-induced Smad signaling. The suppressive effect of the C-Man peptides on the TGF-β-induced Smad signaling is regulated through the enhanced interaction between Smads and Hsc70, suggesting a novel inhibitory effect of C-Man peptides on TGF-β-induced cellular proliferation signaling in the cells.
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