| Project/Area Number |
24570141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 共生細菌 / グリセロ糖脂質 / スフィンゴ糖脂質 / 自然抗体 / TLC-免疫染色 / 細菌受容体糖脂質 / GA1のフコシル化 / ABO血液型ヒト血清 / 糖脂質 / フコース転移酵素 / 消化管上皮 / 硫酸化糖脂質 / フコシル化糖脂質 / 免疫不全マウス / 共生乳酸菌 / 細菌受容体 / 免疫不全 / スルホ糖脂質 |
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| Outline of Final Research Achievements |
Intestinal lactobacilli in immune-deficient scid mice were distinct from those in control mice, i.e. L. murinus (LM) for scid and L. johnsonii (LJ) for control, respectively, which were closely related with the loose and solid properties of the caecal and colonal contents of scid and control mice, respectively. LJ contained LacTH-DG and LacTetH-DG, both of which were modified LacDH-DG with Galα1-6 linkage, whereas, LM contained LacβTH-DG modified LacDH-DG with Glcβ1-6 linkage. Since natural antibodies toward LacTH-DG and LacTetH-DG were frequently detected in human sera, irrespective of ABO-blood groups, in the similar intensities as those toward blood groups- and species-specific glycolipids, immune system is involved in the establishment of symbiotic relationship with bacteria in the intestine. On the other hand, enhanced fucosylation of GA1 as a receptor for LJ occurred in the digestive tracts of scid mice, probably to interfere the formation of bacterial flora of LJ by LM.
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