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Analyses of inhibitory effects of Inhibitory factor-1 on human ATP-synthase.

Research Project

Project/Area Number 24570149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionThe University of Tokyo (2014)
Tokyo Institute of Technology (2012-2013)

Principal Investigator

SUZUKI Toshiharu  東京大学, 工学(系)研究科(研究院), 主幹研究員 (60618809)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsATPase / F1-ATPase / single molecule analysis / ATP / FoF1-ATP合成酵素 / human / motor protein / 反応素過程
Outline of Final Research Achievements

Firstly, I analyzed rotary catalysis mechanism of human F1-ATPase by single molecule analyses. The rotation scheme revealed was that ATP-binding, Pi-releasing and ATP-hydrolysis steps of the ATP-hydrolysis turnover occur at 0, 65 and 90 deg, which was different from bacterial one and enables us to explain the functional state of crystal structures of bovine F1 so far reported. Then, inhibitory factor-1 (IF1) was shown to prevent the rotation at 90 deg, and the inhibited state was removed only by forced rotation of F1 with magnetic tweezer at the direction of ATP-synthesis : F1 was broken when forced rotation at ATP-hydrolysis direction. These results support and explain earlier findings from crystal structures and biochemical analysis that IF1 blocks only ATP-hydrolysis reaction by preventing an elementary step of ATP-hydrolysis by preventing rotation. These results would provide important clues to understand the regulatory mechanism of IF1 for future applied purposes.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 4 results) Book (1 results)

  • [Journal Article] Chemo-mechanical coupling of human mitochondrial F1-ATPase motor.2014

    • Author(s)
      Suzuki T*, Tanaka K, Wakabayashi C, Saita E, Yoshida M
    • Journal Title

      Nature Chem Biol

      Volume: 10 Issue: 11 Pages: 930-936

    • DOI

      10.1038/nchembio.1635

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Vesicular Glutamate Transporters use flexible anion and cation binding sites for efficient accumulation of neurotransmitter.2014

    • Author(s)
      Preobraschenski, J, Zender, JF, Suzuki, T, and Jahn, R
    • Journal Title

      Neuron

      Volume: 84 Issue: 6 Pages: 1287-301

    • DOI

      10.1016/j.neuron.2014.11.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of intra-mitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation2013

    • Author(s)
      Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Tkafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y and Takashima S
    • Journal Title

      PNAS

      Volume: 111 Issue: 1 Pages: 273-278

    • DOI

      10.1073/pnas.1318547111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 生命のATPシステムを作り出す、FoF1-ATP合成酵素の分子機構2014

    • Author(s)
      鈴木俊治
    • Organizer
      東京大学 講演会
    • Place of Presentation
      東京大学・本郷
    • Year and Date
      2014-06-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Single molecule analyses of human F1-ATPase revealed distinct rotation scheme of mitochondrial F1 motor.2014

    • Author(s)
      Toshiharu Suzuki
    • Organizer
      Tokyo ATPase Workshop
    • Place of Presentation
      東京大学・本郷
    • Year and Date
      2014-06-02 – 2014-06-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 生命のATPシステムを作り出すFoF1-ATP合成酵素2014

    • Author(s)
      鈴木俊治
    • Organizer
      大阪大学 医学部講演会
    • Place of Presentation
      大阪大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Single molecule analyses of human F1-ATPase revealed distinct rotation scheme of mitochondrial F1 motor.2013

    • Author(s)
      Toshiharu Suzuki, Kazumi Tanaka, Chiaki Wakabayashi, Shou Furuike, Eiichiro Saita, Kazuhiko Kinosita, Masasuke Yoshida
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトF1-ATPaseの一分子解析が明らかにした、バクテリアとは異なったミトコンドリアF1の回転スキーム2013

    • Author(s)
      鈴木俊治、田中一巳、若林千晃、古池 晶、税田英一郎、木下一彦、吉田賢右
    • Organizer
      日本生体エネルギー研究会第39開討論会
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] Single-molecule analyses of the rotation and regulation of human F1-ATPase2012

    • Author(s)
      Toshiharu Suzuki, Tanaka Kazumi, Chiaki Wakabayashi, Eiichiro Saita, Shou Furuike, Kazuhiko Kinosita Jr, Masasuke Yoshida
    • Organizer
      日本生物物理学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒトF1-ATPaseの回転・制御機構2012

    • Author(s)
      鈴木俊治
    • Organizer
      分子モーター討論会
    • Place of Presentation
      東大・本郷
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ヒトF1-ATPaseの回転と制御の一分子解析2012

    • Author(s)
      鈴木俊治、田中一巳、若林千晃、古池 晶、税田英一郎、木下一彦、吉田賢右
    • Organizer
      日本生化学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Book] 実験医学増刊「構造生命科学で何がわかるのか、何ができるのか」2014

    • Author(s)
      鈴木俊治、吉田賢右
    • Total Pages
      150
    • Publisher
      羊土社
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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