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Resolving the function of the ENH1-PKC/PKD-Calcium Channel function in the cardiac hypertrophy

Research Project

Project/Area Number 24570150
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionNagoya University

Principal Investigator

MATURANA Andres  名古屋大学, 生命農学研究科, 准教授 (10452004)

Co-Investigator(Renkei-kenkyūsha) KURODA Shunichi  名古屋大学, 大学院生命農学研究科, 教授 (60263406)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsシグナル伝達 / 心臓肥大 / intracellular signaling / scaffolding protein / cardiac hypetrophy / PDZ LIM protin / 細胞内シグナル / Zでぃすく / 足場タンパク質 / Zディスク
Outline of Final Research Achievements

Scaffolding proteins are essential for the transmission of intracellular signaling. Scaffolding proteins gather signaling proteins and their targets at precise subcellular locations to maximize the efficiency of the signal transduction. We are studying a scaffolding protein called ENH that is essential for the cardiac and skeletal muscle development. We have previously shown that ENH is essential for the development of cardiac hypertrophy but some ENH splice variants can on the contrary prevent cardiac hypertrophy. In the present study, we studied the splicing mechanisms of ENH. We generate a splice variant ENH trangenic mice to study in-vivo its ability to prevent cardiac hypertrophy. Finally, we analyzed the downstream signaling events downstream to the complex signaling composed by ENH1, the protein kinase D and the L-type calcium channels. Our work sustain the idea that ENH is a key player in the cardiac remodeling leading to heart hypertrophy.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report

Research Products

(20 results)

All 2014 2013 2012

All Journal Article Presentation

  • [Journal Article] Steroid hormones’ genomic and non-genomic actions on cardiac voltage-gated calcium channels2014

    • Author(s)
      Ito J, Andrés Daniel Maturana
    • Journal Title

      Folia Pharmacol. Jpn.

      Volume: 144 Issue: 5 Pages: 206-2010

    • DOI
      10.1254/fpj.144.206
    • NAID
      130004704233
    • Related Report
      2014 Annual Research Report
  • [Journal Article] Enigma homolog 1 promotes myogenic gene expression and differentiation of C2C12 cells.2014

    • Author(s)
      Ito J, Takita M, Takimoto K, Maturana AD
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 289 Pages: 483-487

    • DOI
      10.1016/j.bbrc.2013.05.016
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the counter-transport mechanism of a H+/Ca2+ exchanger.2014

    • Author(s)
      Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O
    • Journal Title

      Science

      Volume: 341 Pages: 168-172

    • DOI
      10.1126/science.1239002
    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system2014

    • Author(s)
      Yoshimoto N, Tatematsu K, Iijima M, Niimi T, Maturana AD, Fujii I, Kondo A, Tanizawa K, Kuroda S
    • Journal Title

      Scientific Reports

      Volume: 4 Pages: 4242-4242

    • DOI
      10.1038/srep04242
    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] DHEA prevents mineralo- and glucocorticoid receptor-induced chronotropic and hypertrophic actions in isolated rat cardiomyocytes.2013

    • Author(s)
      Mannic T, Mouffok M, Python M, Yoshida T, Maturana AD, Vuilleumier N, Rossier MF
    • Journal Title

      Endocrinology

      Volume: 154 Pages: 1271-1281

    • DOI
      10.1210/en.2012-1784
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cell surface-fluorescence immunosorbent assay for real-time detection of hybridomas with efficient antibody secretion at the single-cell level.2013

    • Author(s)
      Kida, A., et al.
    • Journal Title

      Anal Chem.

      Volume: 85 Pages: 1753-1759

    • DOI
      10.1021/ac303067k
    • Related Report
      2012 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An automated system for high-throughput single cell-based breeding.2013

    • Author(s)
      Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andrés D. Maturana, Itoshi Nikaido, Hiroki R. Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: 3 Pages: 1191-1191

    • DOI
      10.1038/srep01191
    • Related Report
      2012 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural basis for the drug extrusion mechanism by a MATE multidrug transporter.2013

    • Author(s)
      Y・ Tanaka, C・J・ Hipolito, A・D・ Maturana, K・ Ito, T・ Kuroda, T・ Higuchi, T・ Katoh, H・E・ Kato, M・ Hattori, K・ Kumazaki, T・ Tsukazaki, R・ Ishitani, H・ Suga, O・ Nureki
    • Journal Title

      Nature

      Volume: 496 Pages: 247-251

    • DOI
      10.1038/nature12014
    • NAID
      120005716854
    • Related Report
      2012 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Splicing transitions of the anchoring protein ENH during striated muscle development.2012

    • Author(s)
      Ito J, Hashimoto T, Nakamura S, Aita Y, Yamazaki T, Schlegel W, Takimoto K, Maturana AD
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 421 Pages: 232-238

    • DOI
      10.1016/j.bbrc.2012.03.142
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Regulation of CREB phosphorylation by scaffolding protein ENH1 in neonatal rat cardiomyocytes2014

    • Author(s)
      Ito J, Kuroda S, Maturana AD
    • Organizer
      the 15th IUBMB-24th FAOBMB-TSBMB International Conference
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2014-10-21 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] The scaffolding protein ENH1 recruits Id2 to Z-discs enabling cardiomyocytes remodelingns on cardiac voltage-gated calcium channels2014

    • Author(s)
      Ito J, Kuroda S, Maturana AD
    • Organizer
      第78回日本生化学会中部支部例会
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Splicing transitions of the anchoring protein ENH during striated muscle development2014

    • Author(s)
      Ito J, Kuroda S, Takimoto K, Maturana AD
    • Organizer
      第61回 日本生化学会近畿支部例会
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Inhibitor of differentiation/DNA binding protein 2 regulates the expression of the cardiac T-type calcium channels2014

    • Author(s)
      Andres D. Maturana
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台国際センター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ENH1 scaffolds Id2 at Z-disk to promote cardiac hypertrophy2013

    • Author(s)
      Ito, J., Kuroda, S., and Maturana, A.D.
    • Organizer
      EMBL conference: Myofibrillar Z-disk structure and Dynamics
    • Place of Presentation
      Hamburg, Germany
    • Related Report
      2013 Research-status Report
  • [Presentation] Enigma Homolog 1 promotes myogenic gene expression and differentiation of C2C12 cell2013

    • Author(s)
      Jumpei Ito, Koichi Takimoto, Shun'ichi Kuroda, Andres D. Maturana
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 転写制御因子Id2は心臓のカルシウムチャネルの発現を制御する2013

    • Author(s)
      伊藤淳平、ヴォルチ・セバスチャン、滝本浩一、マツラナ・アンドレス、
    • Organizer
      生化学若い研究者の会第53回生命科学夏の学校
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 足場タンパク質ENH1は転写抑制因子Id2をZ-diskに留めることにより心肥大を促進する2013

    • Author(s)
      伊藤淳平、黒田俊一、マツラナ・アンドレス
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] The transcriptional regulator, Id2, regulates the cardiac calcium channel expression2013

    • Author(s)
      Ito J, Walchli S, Takimoto K, Maturana AD
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Inhibitor of differentiation /DNA binding protein, Id2, prevents the expression and activity of cardiac calcium channels2012

    • Author(s)
      Ito J, Walchli S, Takimoto K, Maturana AD
    • Organizer
      13th FAOBMB (Federation of Asian and Oceania Biochemists and Molecular Biologists)
    • Place of Presentation
      バンコク、タイ
    • Related Report
      2012 Research-status Report
  • [Presentation] . Inhibitor of differentiation /DNA binding protein, Id2, prevents the expression and activity of cardiac T-type calcium channels2012

    • Author(s)
      Ito J, Walchli S, Takimoto K, Maturana AD
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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