• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of a chaperone/protease involved in quality control of bacterial cell surface

Research Project

Project/Area Number 24570152
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionMorioka College (2013-2014)
Kyoto University (2012)

Principal Investigator

NARITA Shin-ichiro  盛岡大学, 栄養学部, 准教授 (30338751)

Co-Investigator(Kenkyū-buntansha) AKIYAMA Yoshinori  京都大学, ウイルス研究所, 教授 (10192460)
Research Collaborator DAIMON Yasushi  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords細菌細胞表層 / 表層ストレス応答 / σE / 外膜タンパク質 / リポ多糖 / プロテアーゼ / ジスルフィド結合 / 大腸菌 / タンパク質品質管理 / シグマE / BAM複合体
Outline of Final Research Achievements

Escherichia coli is equipped with envelope stress-response systems to sense and cope with disorders of outer membrane (OM) constituents. The σE-dependent stress response system is one of the most important for the maintenance of the OM structure and function. Disruption of yfgC, a σE-regulated gene, sensitizes cells to multiple drugs, suggesting that it is involved in maintaining OM integrity. However, the specific function of YfgC remained unclear. We revealed that YfgC promotes assembly of LptD, an OM protein involved in the transport of lipopolysaccharides, which undergoes intramolecular disulfide rearrangement during its biogenesis. YfgC also promoted degradation of incorrectly folded LptD. BepA thus controls the quality of OM proteins by promoting either the biogenesis or elimination of OM proteins, depending on their folding state. Based on these results, we suggested that YfgC should be renamed as BepA (β-barrel assembly-enhancing protease).

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (10 results) Remarks (5 results)

  • [Journal Article] Small molecule inhibitors of Gram-negative lipoprotein trafficking discovered by phenotypic screening.2015

    • Author(s)
      McLeod, S. M., Fleming, P. R., MacCormack, K., McLaughlin, R. E., Whiteaker, J. D., Narita, S., Mori, M., Tokuda, H., and Miller, A. A.
    • Journal Title

      J. Bacteriol.

      Volume: 197 Issue: 6 Pages: 1075-1082

    • DOI

      10.1128/jb.02352-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel antibacterial targets and compounds revealed by a high throughput cell wall reporter assay2015

    • Author(s)
      Asha S. Nayar, Thomas J. Dougherty, Keith E. Ferguson, Brett A. Granger, Lisa McWilliams, Clare Stacey, Lindsey J. Leach, Shin-ichiro Narita, Hajime Tokuda, Alita A. Miller, Dean G. Brown, and Sarah M. McLeod
    • Journal Title

      J. Bacteriol.

      Volume: 197 Issue: 10 Pages: 1726-1734

    • DOI

      10.1128/jb.02552-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of the protruding loop of factor B essential for the localization of lipoproteins (LolB) in the anchoring of bacterial triacylated proteins to the outer membrane.2014

    • Author(s)
      Hayashi, Y., Tsurumizu, R., Tsukahara, J., Takeda, K., Narita, S., Mori, M., Miki, K., and Tokuda, H.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 15 Pages: 10530-10539

    • DOI

      10.1074/jbc.m113.539270

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Protease homolog BepA (YfgC) promotes assembly and degradation of β-barrel membrane proteins in Escherichia coli.2013

    • Author(s)
      Shin-ichiro Narita, Chigusa Masui, Takehiro Suzuki, Naoshi Dohmae and Yoshinori Akiyama
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: 110 Issue: 38

    • DOI

      10.1073/pnas.1312012110

    • NAID

      120005323037

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional differentiation of structurally similar membrane subunits of the ABC transporter LolCDE complex.2013

    • Author(s)
      Mizutani, M., Mukaiyama, K., Xiao, J., Mori, M., Satou, R., Narita, S., Okuda, S., and Tokuda, H.
    • Journal Title

      FEBS Lett.

      Volume: 587 Issue: 1 Pages: 23-29

    • DOI

      10.1016/j.febslet.2012.11.009

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Defective lipoprotein sorting induces lolA expression through the Rcs stress response phosphorelay system.2012

    • Author(s)
      Tao, K., Narita, S., and Tokuda, H.
    • Journal Title

      J. Bacteriol.

      Volume: (in press) Issue: 14 Pages: 3643-3650

    • DOI

      10.1128/jb.00553-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] σEの欠失による大腸菌の致死性はTAシステムトキシンの活性化により抑制される2015

    • Author(s)
      大門康志、成田新一郎、秋山芳展
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大門康志、成田新一郎. 大腸菌の表層ストレス応答に関わるσEの必須性はTA システムにおけるtoxinの活性化により解消される2015

    • Author(s)
      大門康志、成田新一郎
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場
    • Year and Date
      2015-03-26 – 2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸菌プロテアーゼBepAは外膜タンパク質の組立と分解を促進する2013

    • Author(s)
      成田新一郎、舛井千草、鈴木健裕、堂前直、秋山芳展
    • Organizer
      日本農芸化学会東北支部 第148回大会
    • Place of Presentation
      岩手大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 部位特異的in vivo光架橋法による大腸菌ペリプラズムタンパク質BepA(YfgC)の近接因子探索2013

    • Author(s)
      舛井千草、成田新一郎、秋山芳展
    • Organizer
      第10回 21世紀大腸菌研究会
    • Place of Presentation
      静岡県伊豆市
    • Related Report
      2013 Research-status Report
  • [Presentation] 大腸菌βバレル型膜タンパク質の生合成に関わるBepA (YfgC)の機能解析2013

    • Author(s)
      成田新一郎、鈴木健裕、堂前直、秋山芳展
    • Organizer
      日本農芸化学会2013年度(平成25年度)大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸菌βバレ ル型タンパク質の品質管理に関わるプロテアーゼホモログBepA (YfgC)の機能解析2013

    • Author(s)
      成田新一郎、秋山芳展
    • Organizer
      2012年度国立遺伝学研究所研究会「単細胞システムの構築とその維持機構の研究」
    • Place of Presentation
      三島
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸菌におけるσE依存性表層ストレス応答とtoxin-antitoxin systemの関わり2012

    • Author(s)
      大門康志、成田新一郎、秋山芳展
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      長浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Characterization of YfgC, a protease homolog involved in assembly of the Escherichia coli outer membrane proteins2012

    • Author(s)
      Shin-ichiro Narita and Yoshinori Akiyama
    • Organizer
      Gordon Research Conference on Bacterial Cell Surfaces
    • Place of Presentation
      West Dover, VT, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸菌外膜タンパク質の品質管理にかかわる新規プロテアーゼホモログBepAの解析2012

    • Author(s)
      成田新一郎、秋山芳展
    • Organizer
      2012年度国立遺伝学研究所研究会「代謝、増殖、分裂研究会」
    • Place of Presentation
      三島
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸菌におけるσEの必須性とtoxin-antitoxinシステムの関わり2012

    • Author(s)
      大門康志、成田新一郎、志波優、吉川博文、秋山芳展
    • Organizer
      2012年度国立遺伝学研究所研究会「代謝、増殖、分裂研究会」
    • Place of Presentation
      三島
    • Related Report
      2012 Research-status Report
  • [Remarks] 京都大学ウイルス研究所 研究所紹介 最新の研究成果

    • URL

      http://www.virus.kyoto-u.ac.jp/about/y2013/akiyama201309.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 京都大学 膜タンパク質の組立と分解に関わる新規プロテアーゼを発見

    • URL

      http://goo.gl/mCP2hH

    • Related Report
      2014 Annual Research Report
  • [Remarks] 盛岡大学栄養科学部教員総覧

    • URL

      http://www.morioka-u.ac.jp/UV_ns/kyouin/narita_shinichiro.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 膜タンパク質の組立と分解に関わる新規プロテアーゼを発見

    • URL

      http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013/130903_1.htm

    • Related Report
      2013 Research-status Report
  • [Remarks] BepA (YfgC)は大腸菌βバレル型タンパク質のアセンブリーと分解を促進する

    • URL

      http://www.virus.kyoto-u.ac.jp/about/y2013/akiyama201309.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi