Transglutaminase-Catalyzed Protein-Protein Crosslinking Maintains the Gut Epithelial Immunity in Drosophila.
| Project/Area Number |
24570164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIBATA Toshio 九州大学, 高等研究院, 助教 (00614257)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | トランスグルタミナーゼ / ショウジョウバエ / 腸管 / 腸内細菌 / ペプチドグリカン / タンパク質架橋反応 / 腸内細菌叢 / 囲食膜 / キチン結合タンパク質 / 情報伝達経路 / 転写因子 / 抗菌ペプチド / 自然免疫 / 腸管免疫 / 常在細菌叢 / 共生 / 架橋酵素 / シグナル伝達制御 |
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| Outline of Final Research Achievements |
RNA interference directed against transglutaminase (TG) caused a short life span in flies reared under conventional non-sterile conditions, but not under germ-free conditions. TG RNAi enhanced the expression of genes encoding antimicrobial peptides in the immune deficiency (IMD) pathway. Wild-type flies that ingested gut lysates prepared from conventionally reared TG RNAi-treated flies had shorter life spans. In conventionally reared flies, TG RNAi triggered apoptosis in the gut and induced the nuclear translocation of Relish, the NF-κB-like transcription factor of the IMD pathway. Wild-type flies that ingested synthetic amine donors, which inhibit the TG-catalyzed protein-protein crosslinking reaction, showed nuclear translocation of Relish and enhanced expression of genes encoding IMD-controlled antimicrobial peptide genes in the gut. We also found that TG enhances the structural strength of the peritrophic matrix by crosslinking chitin-binding proteins.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Transglutaminase-catalyzed protein-protein cross-linking suppresses the activity of the NF-κB-like transcription factor Relish.2013
Author(s)
Shibata, T., Sekihara, S., Fujikawa, T., Miyaji, R., Maki, K., Ishihara, T., Koshiba, T., and Kawabata, S.
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Journal Title
Science Signaling
Volume: 6
Issue: 285
Pages: 1-9
DOI
Related Report
Peer Reviewed
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[Presentation] Transglutaminase-catalyzed protein-protein crosslinking maintains the gut epithelial immunity.2014
Author(s)
Shibata, T., Sekihara, S., Fujikawa, T., Miyaji, R., Maki, K., Ishihara, T., Koshiba, T., Kawabata, S.
Organizer
Gordon Research Conference on Transglutaminase in Human Disease Processes.
Place of Presentation
Lucca, Italy
Year and Date
2014-06-29 – 2014-07-04
Related Report
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