Identification and functional analysis of the GPI-anchored proteins involved in the germline niche formation of the nematode C. elegans
| Project/Area Number |
24570165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
NOMURA Kazuya 九州大学, 理学(系)研究科(研究院), 准教授 (30150395)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | GPIアンカー / 配偶子幹細胞 / 幹細胞ニッチ / 卵母細胞 / 配偶子 / PIGA / CGGDBデータベース / C. elegans / 体細胞分裂 / 減数分裂 / プロテオグリカン / 糖鎖 / 線虫 / 生殖幹細胞 |
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| Outline of Final Research Achievements |
Inhibition of GPI-anchored protein (GPI-AP) synthesis of C. elegans results in severe germline abnormalities. By analyzing a deletion allele of the GPI-anchor synthesizing gene piga-1, we confirmed that the phenotypes were caused by the decrease of dividing germline stem cells (GSCs) at the distal end of the gonad where distal tip cell is providing Notch signal to maintain GSC niche. In this study, we studied GPI-APs enriched in the germline by biochemical analysis, by DNA microarray and RNA-Seq. We also examined RNAi phenotypes of all the GPI-AP genes to identify essential GPI-APs in the GSC niche. From these analyses, we identified seven different GPI-AP coding genes. Among them, two genes seem to be especially important. Thus, we isolated CRISPR-Cas9 mediated deletion allele of the two genes, and found that the germline formation was severely affected in the allele. We are examining the possible involvement of these two genes in the Notch signaling pathways of the GSCs.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.2014
Author(s)
Akiyoshi S, Nomura KH, Dejima K, Murata D, Matsuda A, Kanaki N, Takaki T, Mihara H, Nagaishi T, Furukawa S, Ando KG, Yoshina S, Mitani S, Togayachi A, Suzuki Y, Shikanai T, Narimatsu H, Nomura K.
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Journal Title
Glycobiology
Volume: 25(1)
Issue: 1
Pages: 8-20
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 線虫C. elegansの生殖系列細胞におけるGPIアンカー型タンパク質の解析
Author(s)
松田采子, 村田大輔, 野村和子, 秋好紗弥香, 出嶋克史, Lance Wells, Karen L. Abbott, 川崎 ナナ, 安藤恵子, 中台枝里子, 三谷昌平, 野村一也
Organizer
第36回日本分子生物学会大会
Place of Presentation
神戸
Related Report
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[Presentation] Analysis of GPI-anchored proteins indispensable for the germ cell development in C. elegans
Author(s)
Ayako Matsuda, Daisuke Murata, Kazuko Nomura, Sayaka Akiyoshi, Katsufumi Dejima, Lance Wells, Karen L. Abbott, Nana Kawasaki, Keiko Gengyo-Ando, Eriko Nakadai, Shohei Mitani, Kazuya Nomura
Organizer
The 3rd International Congress on Natural Sciences with Sisterhood Universities (ICNS2013)
Place of Presentation
新潟
Related Report
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[Presentation] GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans
Author(s)
Daisuke Murata, Kazuko H. Nomura, Ayako Matsuda, Sayaka Akiyoshi, Katsufumi Dejima, Lance Wells, Karen L. Abbott, Nana Kawasaki, Keiko Gengyo-Ando, Eriko Kage-Nakadai, Shohei Mitani, Kazuya Nomura
Organizer
GLYCO22 (the 22nd International Glycoconjugate Symposium )
Place of Presentation
大連（中国）
Related Report
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