| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In attempt to search the genes repressed in colorectal cancer-derived cell lines, we found that DGKG expression is strongly suppressed in the cancer cells from both of cell lines and primary tumors. Because DGKG contains a CpG island in the region around its transcription start site, we carried out bisulfite pyrosequencing analysis, resulting that DGKG is strongly methylated in colorectal cancer cells. These findings confirmed the presence of high levels of methylation in most of the cells in which DGKG expression was silenced. The overexpression of DGKG in a DGKG-silenced colorectal cancer cell line, HCT116 decreased the migration and invasion activities of the cells significantly. In addition, DGKG activity modified the activation levels of Rac1 and Rho small GTPase proteins. These results suggested that DGKG regluated cell migration via Rho-family small GTPases. The epigenetic silencing of DGKG may correlate with the cancer malignancy in colorectal cancer.
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