• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanisms on biosynthesis of lysosome-related organelles

Research Project

Project/Area Number 24570167
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionNagoya University (2014)
Fukushima Medical University (2012-2013)

Principal Investigator

KAMETAKA Satoshi  名古屋大学, 医学(系)研究科(研究院), 教授 (10303950)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsライソゾーム / ライソゾーム関連オルガネラ / 色素顆粒 / 光受容神経細胞 / ライソゾーム蓄積症候群 / ライソゾーム病 / サポシン / ショウジョウバエ / レクチン / リポフスチン / クラスリン / 国際情報交換
Outline of Final Research Achievements

Targeting of endosomal or lysosomal proteins to their destinations requires sorting mechanisms at the trans-Golgi network (TGN) in the secretory pathway. The sorting event is also prerequisite for de novo generation of cell-type specific post-Golgi compartments such as lysosome related organelles (LRO). In this study, we found that the Golgi-resident clathrin adaptor proteins play essential roles in synthesis and functional maintenance of lysosomes and LROs using the fruits fly as a model organism.
Flies lacking AP-1 showed enlarged photoreceptor neurons and significant accumulation of pigment granules in the pigment epithelia. Morphological and immunocytochemical analyses suggested that appearance of these aberrant structures are accompanied with missorting of lysosomal proteins, suggesting that the sorting event at the TGN is also required for generation of pigment granules.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] ArfGAP3 regulates the transport of cation-independent mannose 6-phosphate receptor in the post-Golgi compartment2013

    • Author(s)
      Shiba Y, Kametaka S, Waguri S, Presley JF, Randazzo PA
    • Journal Title

      Curr. Biol.

      Volume: 23 Issue: 19 Pages: 1945-1951

    • DOI

      10.1016/j.cub.2013.07.087

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ライソゾーム病関連因子のモデル生物を用いた解析.2014

    • Author(s)
      亀高諭,和栗聡
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      栃木
    • Related Report
      2013 Research-status Report
  • [Presentation] Functional characterization of lysosomal enzyme receptor protein (LERP) in Drosophila2013

    • Author(s)
      Satoshi Kametaka and Satoshi Wagur
    • Organizer
      XXIII International Symposium on morphological Science 2013
    • Place of Presentation
      新潟
    • Related Report
      2013 Research-status Report
  • [Presentation] Functional characterization of lysosomal enzyme receptor protein (LERP) in Drosophila.2013

    • Author(s)
      Satoshi Kametaka
    • Organizer
      International Symposium Anatomical Science for advance in health and clinical therapy 2013 (ASahct 2013)
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] ショウジョウバエを用いたライソゾーム蛋白質輸送機構の解析.2013

    • Author(s)
      亀高諭,上野隆,和栗聡
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report
  • [Presentation] ライソゾーム酵素局在化に関わる選別受容体Lerpの生理機能解析2013

    • Author(s)
      亀高 諭, 和栗 聡
    • Organizer
      第118回日本解剖学会総会
    • Place of Presentation
      高松
    • Related Report
      2012 Research-status Report
  • [Presentation] AP-1 dependent trafficking of Notch during eye development in Drosophila2012

    • Author(s)
      亀高諭、亀高愛、米倉真一、後藤聡、和栗聡
    • Organizer
      Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] AP-1 and Aftiphilin function in the Notch signaling during eye development in Drosophila2012

    • Author(s)
      Satoshi Kametaka, Ai Kametaka, Shinichi Yonekura, Satoshi Goto, Satoshi Waguri
    • Organizer
      Gordon Research Conference - Lysosomes & Endocytosis
    • Place of Presentation
      NH, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] AP-1 and Aftiphilin function in the Notch signaling during eye development in Drosophila2012

    • Author(s)
      Satoshi Kametaka, Ai Kametaka, Shinichi Yonekura, Satoshi Goto, Satoshi Waguri
    • Organizer
      Branch Seminar at NICHD, NIH
    • Place of Presentation
      MD, USA
    • Related Report
      2012 Research-status Report
    • Invited

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi