| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
Targeting of endosomal or lysosomal proteins to their destinations requires sorting mechanisms at the trans-Golgi network (TGN) in the secretory pathway. The sorting event is also prerequisite for de novo generation of cell-type specific post-Golgi compartments such as lysosome related organelles (LRO). In this study, we found that the Golgi-resident clathrin adaptor proteins play essential roles in synthesis and functional maintenance of lysosomes and LROs using the fruits fly as a model organism.
Flies lacking AP-1 showed enlarged photoreceptor neurons and significant accumulation of pigment granules in the pigment epithelia. Morphological and immunocytochemical analyses suggested that appearance of these aberrant structures are accompanied with missorting of lysosomal proteins, suggesting that the sorting event at the TGN is also required for generation of pigment granules.
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