| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
In this study, we generated two hybridomas using a hiPS cell line (Tic) as an antigen. These hybridomas produced antibodies that specifically bound to hiPS/ES cells but exhibited little or no binding to hEC cells. Interestingly, both of these antibodies were found to be carbohydrate–recognizing antibodies of the IgG1 subtype. 1st antibody R-10G recognizes a type of keratan sulfate lacking oversulfated structures expressed on podocalyxin molecules on hiPS/ES cells like anti-TRA-1-60 antibody. 2nd antibody R-17F recognized glycolipids like anti-SSEA-3 antibody, and exhibited a strong cytotoxic effect toward hiPS/ES cells.
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