Generation and functional analysis of monoclonal antibodies raised against human iPS cells
| Project/Area Number |
24570171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Nobuko 立命館大学, 総合科学技術研究機構, 教授 (70077676)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 多能性幹細胞 / 糖鎖認識抗体 / ケラタン硫酸 / 糖脂質 / ES細胞 / iPS細胞 / ヒトiPS 細胞 / ヒトES 細胞 / 細胞傷害性抗体 / フローサイトメトリー / 共焦点レーザー顕微鏡 / 単クローン抗体 / ポドカリキシン / R-!0G / R-17F / ヒトES細胞 / R－10G / R－17F / 低硫酸化ケラタン硫酸 |
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| Outline of Final Research Achievements |
In this study, we generated two hybridomas using a hiPS cell line (Tic) as an antigen. These hybridomas produced antibodies that specifically bound to hiPS/ES cells but exhibited little or no binding to hEC cells. Interestingly, both of these antibodies were found to be carbohydrate–recognizing antibodies of the IgG1 subtype. 1st antibody R-10G recognizes a type of keratan sulfate lacking oversulfated structures expressed on podocalyxin molecules on hiPS/ES cells like anti-TRA-1-60 antibody. 2nd antibody R-17F recognized glycolipids like anti-SSEA-3 antibody, and exhibited a strong cytotoxic effect toward hiPS/ES cells.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Mannan-binding protein, a C-type serum lectin, recognizes primary colorectal carcinomas through tumor-associated Lewis glycans.2014
Author(s)
M. Nonaka, H. Imaeda, S. Matsumoto, BY Ma, N. Kawasaki, E. Mekata, A. Andoh, Y. Saito, T. Tani, Y. Fujiyama, T. Kawasaki.
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Journal Title
J Immunol
Volume: 192
Issue: 3
Pages: 1294-1301
DOI
Related Report
Peer Reviewed
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[Journal Article] A Novel Antibody for Human Induced Pluripotent Stem (hiPS) Cells and Embryonic Stem (ES) Cells Recognizes a Type of Keratan Sulfate Lacking Oversulfated Structures.2013
Author(s)
K Kawabe, D Tateyama, H Toyoda, N Kawasaki, N Hashii, H Nakao, S Matsumoto, M Nonaka, H Matsumura, Y Hirose, A Morita, M Katayama, M Sakuma, N Kawasaki, M Kusuda Furue, T Kawasaki.
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Journal Title
Glycobiology
Volume: 23巻
Issue: 3
Pages: 322-336
DOI
Related Report
Peer Reviewed
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[Presentation] Ｃ-型レクチンと免疫
Author(s)
川嵜敏祐
Organizer
Glyco-Immunology 2014
Place of Presentation
東京医科歯科大学 (東京都）
Related Report
Invited
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[Presentation] A novel marker antibody of human induced Pluripotent Stems (iPS) cells, which recognizes a new type of keratan sulfate
Author(s)
Toshisuke Kawasaki, Keiko Kawabe, Miho Kusuda Furue, Hiromi Nakao, Shogo Matsumoto, Motohiro Nonaka, Hidenao Toyoda, Yoshinori Hirose, Nana Kawasaki, and Nobuko Kawasaki
Organizer
International Carbohydrate Symposium 2012
Place of Presentation
Madrid, Spain
Related Report
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[Presentation] ヒトiPS細胞上のケラタン硫酸鎖を認識する新規単クローン抗体の性質
Author(s)
松本尚悟,中尾広美, 河邊圭子, 館山大揮, 廣瀬佳則, 森田彩葉, 野中元裕, 川崎ナナ, 橋井則貴, 川嵜伸子, 古江－楠田美保, 豊田英尚, 川嵜敏祐
Organizer
第31回日本糖質学会年会
Place of Presentation
鹿児島市民文化ホール, 鹿児島県
Related Report
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