| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
Post-translaional modification of RNA is one of the important features of RNA molecules. In this study, the biosynthesis pathway of thiouridine found in tRNA of thermophilic bacteria was analyzed. An in vitro reconstitution system for tRNA thiolation reaction suitable for the quantitative analysis was constructed. By using this system and information of the three-dimensional structure of the thiolation enzyme, a novel reaction mechanism for the thiolation reaction was suggested.
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