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Regulation of Bach2 intrinsically disordered region by ligand

Research Project

Project/Area Number 24570175
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

MURAYAMA Kazutaka  東北大学, 医工学研究科, 准教授 (40400452)

Co-Investigator(Renkei-kenkyūsha) MUTO Akihiko  東北大学, 大学院医学系研究科, 准教授 (80343292)
MATSUI Miki (WATANABE Miki)  東北大学, 大学院医学系研究科, 助教 (00455784)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsタンパク質構造変化 / 天然変性タンパク質 / ヘム結合 / 構造変化 / X線小角散乱
Outline of Final Research Achievements

Bach2 plays an important role in the transcriptional process. Bach2 includes long intrinsically disordered regions. We prepared the region of 331-520 to investigate its biophysical properties. Spectroscopic measurements revealed that Bach2(331-520) is intrinsically disordered region, in which heme binds. SAXS and dynamic light scattering mesurements indicated that heme binding form of Bach2(331-520) is unstabler than the non heme binding form and the structure will be more compact. Our results showed the first example of the heme binding protein which is (1) intrinsically disordered protein/region, (2) indicates structural change by heme binding.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Heme binds to an intrinsically disordered region of Bach2 and alters its conformation2014

    • Author(s)
      M. Watanabe-Matsui, T. Matsumoto, T. Matsui, M. Ikeda-Saito, A. Muto, K. Murayama, and K. Igarashi
    • Journal Title

      Archives of Biochemistry and Biophysics

      Volume: 565巻 Pages: 25-31

    • DOI

      10.1016/j.abb.2014.11.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ヘムによる天然変性タンパク質Bach2の制御2014

    • Author(s)
      渡部(松井)美紀、村山和隆、松井敏高、齋藤正男、五十嵐和彦
    • Organizer
      日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヘムに制御される天然変性タンパク質Bach2の制御機構の解明2013

    • Author(s)
      渡部-松井美紀、村山和隆、松井敏高、齋藤正男、五十嵐和彦
    • Organizer
      日本蛋白質科学会
    • Place of Presentation
      とりぎん文化会館(鳥取市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Heme binding region of Bach2 as intrinsically disordered protein2013

    • Author(s)
      村山和隆、渡部‐松井美紀、五十嵐和彦
    • Organizer
      日本生物物理学会
    • Place of Presentation
      京都国際会館(京都市)
    • Related Report
      2013 Research-status Report
  • [Presentation] X線小角散乱によるBach2タンパク質の構造変化の解析2012

    • Author(s)
      村山和隆、渡部-松井美紀、松本崇、五十嵐和彦
    • Organizer
      日本蛋白質科学会年会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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