| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Outline of Final Research Achievements |
Hydration water is closely involved in the stability and dynamics of protein and plays a vital role in the mechanism of protein function. Here we develop a technique to analyse physical properties of the hydration water around protein and apply this technique to actin, a muscle contractile protein. A fluorescent dye is conjugated to actin through a flexible linker so that the dye is tethered in the hydration layer, and its time-resolved fluorescence polarisation is measured by a newly in-house built high-precision frequency-domain fluorometer that uses an LN light modulator to generate modulated excitation light. The dye undergoes rotational Brownian motion in the hydration layer of actin that can be analysed by the measurement, and the analysis reveals a hydrodynamic property of the hydration layer around actin. The technique developed in this study is useful to move a step closer to understanding the role of hydration water around protein.
|
