Single-molecule direct visualization of DNA repair proteins on chromatin
Project/Area Number |
24570182
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | The Graduate School for the Creation of New Photonics Industries (2013-2014) Kyoto University (2012) |
Principal Investigator |
YOKOTA Hiroaki 光産業創成大学院大学, 光産業創成研究科, 准教授 (90415547)
|
Co-Investigator(Renkei-kenkyūsha) |
SUGASAWA Kaoru 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 教授 (70202124)
IWAI Shigenori 大阪大学, 基礎工学研究科, 教授 (10168544)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | DNA損傷・修復 / タンパク質 / 核酸の構造 / 動態 / 機能 |
Outline of Final Research Achievements |
To elucidate how mammalian (human) DNA repair proteins recognize base lesions, we performed single-molecule direct visualization of the proteins on DNA. We found that XPC-RAD23B protein complex, which is known to be responsible for damage recognition in mammalian nucleotide excision repair, has high affinity for a lesion and performed one-dimensional bidirectional diffusion on undamaged DNA. The diffusion coefficients of the movement indicate that the protein complex does not always perform rotational tracking of the helical pitch of the DNA while moving along DNA and thus diffuses at much higher rates. The obtained results indicate that the protein complex uses these binding modes for efficient search of DNA lesions scattered throughout the genome from a vast excess of normal bases.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Construction and Characterization of Cy3- or Cy5-Conjugated Hairpin Pyrrole / Imidazole Polyamide Binding to DNA in the Nucleosome2014
Author(s)
Han, Y-W.; Tsunaka, Y.; Yokota, H.; Matsumoto, T.; Kashiwazaki, G.; Morinaga, H.; Hashiya, K.; Bando, T.; Sugiyama, H.; Harada, Y.
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Journal Title
Biomater. Sci.
Volume: 2
Issue: 3
Pages: 297-307
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Optically detected magnetic resonance of nanodiamonds in vivo; Implementation of selective imaging and fast sampling2014
Author(s)
Yoshinari, Y., Mori, S., Igarashi, R., Sugi, T., Yokota, H., Ikeda, K., Sumiya, H., Mori, I., Tochio, H., Harada, Y., Shirakawa, M.
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Journal Title
Journal of Nanoscience and Nanotechnology
Volume: -
Related Report
Peer Reviewed
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[Journal Article] Real-time background-free selective imaging of fluorescent nanodiamond in vivn2012
Author(s)
Ryuji Igarashi, Yohsake Yoshinari, Riroaki Yokoia, Takuma Sugi, Fuminori Sugihara, Kazuhiro Ikeda, Hiroshi Sumiya, Shigenori Tsuji, Ikme Mori, Hidehito Tochio, Yoshie Rarada, Masahiro Shirakawa.
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Journal Title
Nano Letters
Volume: 12
Issue: 11
Pages: 5726-5732
DOI
Related Report
Peer Reviewed
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