Analysis of molecular mechanisms to control two electron transfer directions in photosynthetic reaction centers
| Project/Area Number |
24570183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
Oh-oka Hirozo 大阪大学, 理学(系)研究科(研究院), 准教授 (30201966)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 光合成 / 反応中心 / 緑色イオウ細菌 / ヘリオバクテリア / ホモダイマー / 光化学系I / フーリエ赤外分光法 / 電子スピン共鳴 / タイプ１反応中心 / ヘテロダイマー / 電荷再結合 / 電子移動 / ESR / 光化学系１ / アンテナドメイン / エネルギー移動 |
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| Outline of Final Research Achievements |
We mutated PscA-Leu688 and PscA-Val689 to cysteine residues in the green sulfur bacteriumChlorobaculum tepidum; these residues were predicted to interact with the special pair P840, based on sequence comparison with PS I. Light-induced Fourier transform infrared difference measurements showed that the L688C mutation induced a differential signal of the S-H stretching vibration in the P840+/P840 spectrum, as reported in P800+/P800 difference spectrum in a heliobacterial RC. This suggests that there is a common spatial configuration around the special pair sites among type 1 RCs.
We identified a new transient electron spin-polarized electron paramagnetic resonance (ESP-EPR) signal, arising from the radical pair of the oxidized electron donor and the reduced electron acceptor (P800+ MQ－), in the heliobacterial RC (hRC) core complex. The result revealed the quinone usage as the secondary electron acceptor in hRC, as in the case of PS I.
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Report
(5 results)
Research Products
(43 results)
