| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We mutated PscA-Leu688 and PscA-Val689 to cysteine residues in the green sulfur bacteriumChlorobaculum tepidum; these residues were predicted to interact with the special pair P840, based on sequence comparison with PS I. Light-induced Fourier transform infrared difference measurements showed that the L688C mutation induced a differential signal of the S-H stretching vibration in the P840+/P840 spectrum, as reported in P800+/P800 difference spectrum in a heliobacterial RC. This suggests that there is a common spatial configuration around the special pair sites among type 1 RCs.
We identified a new transient electron spin-polarized electron paramagnetic resonance (ESP-EPR) signal, arising from the radical pair of the oxidized electron donor and the reduced electron acceptor (P800+ MQ－), in the heliobacterial RC (hRC) core complex. The result revealed the quinone usage as the secondary electron acceptor in hRC, as in the case of PS I.
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