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Analysis of molecular mechanisms to control two electron transfer directions in photosynthetic reaction centers

Research Project

Project/Area Number 24570183
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

Oh-oka Hirozo  大阪大学, 理学(系)研究科(研究院), 准教授 (30201966)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords光合成 / 反応中心 / 緑色イオウ細菌 / ヘリオバクテリア / ホモダイマー / 光化学系I / フーリエ赤外分光法 / 電子スピン共鳴 / タイプ1反応中心 / ヘテロダイマー / 電荷再結合 / 電子移動 / ESR / 光化学系1 / アンテナドメイン / エネルギー移動
Outline of Final Research Achievements

We mutated PscA-Leu688 and PscA-Val689 to cysteine residues in the green sulfur bacteriumChlorobaculum tepidum; these residues were predicted to interact with the special pair P840, based on sequence comparison with PS I. Light-induced Fourier transform infrared difference measurements showed that the L688C mutation induced a differential signal of the S-H stretching vibration in the P840+/P840 spectrum, as reported in P800+/P800 difference spectrum in a heliobacterial RC. This suggests that there is a common spatial configuration around the special pair sites among type 1 RCs.
We identified a new transient electron spin-polarized electron paramagnetic resonance (ESP-EPR) signal, arising from the radical pair of the oxidized electron donor and the reduced electron acceptor (P800+ MQ-), in the heliobacterial RC (hRC) core complex. The result revealed the quinone usage as the secondary electron acceptor in hRC, as in the case of PS I.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (43 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (34 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Mutation-induced perturbation of the special pair P840 in the homodimeric reaction center in green sulfur bacteria2016

    • Author(s)
      C. Azai, Y. Sano, Y. Kato, T. Noguchi and H. Oh-oka
    • Journal Title

      Sci. Rep.

      Volume: ー Issue: 1 Pages: 19878-19878

    • DOI

      10.1038/srep19878

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Menaquinone as the secondary electron acceptor in the type I homodimeric photosynthetic reaction center of Heliobacterium modesticaldum2015

    • Author(s)
      T. Kondo, S. Itoh, M. Matsuoka, C. Azai and H. Oh-oka
    • Journal Title

      J. Phys. Chem. B

      Volume: 119 Issue: 27 Pages: 8480-8489

    • DOI

      10.1021/acs.jpcb.5b03723

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structure analysis and characterization of the cytochrome c-554 from thermophilic green sulfur photosynthetic bacterium Chlorobaculum tepidum2013

    • Author(s)
      L-J. Yu, M. Unno, Y. Kimura, K. Yanagimoto, H. Oh-oka and Z-Y. Wang-Otomo
    • Journal Title

      Photosynth. Res.

      Volume: 118 Issue: 3 Pages: 249-258

    • DOI

      10.1007/s11120-013-9922-2

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene expression system in the green sulfur bacterium Cholrobaculum tepidum by conjugative plasmid transfer2013

    • Author(s)
      C. Azai, J. Harada, H. Oh-oka
    • Journal Title

      PLoS ONE

      Volume: 8(11) Issue: 11 Pages: e82345-e82345

    • DOI

      10.1371/journal.pone.0082345

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ヘリオバクテリア光合成反応中心の低温蛍光解析2016

    • Author(s)
      大岡宏造、小島理沙、浅井智広、武藤梨沙、栗栖源嗣、伊藤繁
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学・上田キャンパス(岩手)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヘリオバクテリア由来の1型光合成反応中心の活性測定2016

    • Author(s)
      安田亜矢、武藤梨沙、大岡宏造、浅井智広、栗栖源嗣
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学・上田キャンパス(岩手)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Homodimeric type 1 reaction centers in green sulfur bacteria and heliobacteria2016

    • Author(s)
      H. Oh-oka
    • Organizer
      IPR International Workshop
    • Place of Presentation
      大阪大学蛋白質研究所(大阪)
    • Year and Date
      2016-02-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 多孔性シリカ粒子のナノ空間に埋め込まれたヘリオバクテリア反応中心コアタンパクの安定性と分光学的特性2015

    • Author(s)
      大岡宏造、野地智康、浅井智広、武藤梨沙、栗栖源嗣、伊藤繁
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学・角間キャンパス(石川)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] A Rieske/cytochrome b complex in the green sufur bacterium Chlorobaculum tedium2015

    • Author(s)
      H. Oh-oka, T. Yamamoto, R. Mutoh, C. Azai and G. Kurisu
    • Organizer
      15th International Symposium on Phototrophic Prokaryotes
    • Place of Presentation
      Tubingen, Germany
    • Year and Date
      2015-08-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacteriochlorophyll a synthesis in the green sulfur bacterium Chlorobaculum tepidum mutant lacking BchX, a subunit of chlorophyllide a oxidoreductase2015

    • Author(s)
      C. Azai, T. Mizoguchi, H. Tamiaki and H. Oh-oka
    • Organizer
      15th International Symposium on Phototrophic Prokaryotes
    • Place of Presentation
      Tubingen, Germany
    • Year and Date
      2015-08-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Heliobacterial type-I homodimer reaction center uses menaquinone as the secondary electron acceptor at a little different location from that of phylloquinone in PSI2015

    • Author(s)
      S. Itoh, T. Kondo, C. Azai and H. Oh-oka
    • Organizer
      15th International Symposium on Phototrophic Prokaryotes
    • Place of Presentation
      Tubingen, Germany
    • Year and Date
      2015-08-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多孔性シリカ粒子中のナノ空間に安定的に保持されたヘリオバクテリア反応中心2015

    • Author(s)
      大岡宏造、野地智康、浅井智広、武藤梨沙、栗栖源嗣、伊藤繁
    • Organizer
      第6回日本光合成学会年会および公開シンポジウム
    • Place of Presentation
      岡山国際交流センター(岡山)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] ホモダイマー光合成反応中心複合体の人工的ヘテロダイマー化2015

    • Author(s)
      浅井智広、Kwang Kim、近藤徹、原田二朗、伊藤繁、大岡宏造
    • Organizer
      第62回日本生化学会近畿支部例会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀)
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] タイプ1光合成反応中心の保存されたアンテナクロロフィルの役割2015

    • Author(s)
      浅井智広、近藤徹、伊藤繁、大岡宏造
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学・世田谷キャンパス(東京)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 緑色イオウ光合成細菌のシトクロムbc複合体はRieske/cytochrome bタイプである2015

    • Author(s)
      大岡宏造、山本和矢、武藤梨沙、浅井智広、栗栖源嗣
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学・世田谷キャンパス(東京)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] ホモダイマー光合成反応中心の反応特性と電子移動経路を探る2015

    • Author(s)
      大岡宏造
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所1階講堂(大阪)
    • Year and Date
      2015-03-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Isolation of Rieske/cytochrome b complex from a green sulfur bacterium Chlorobaculum tepidum2014

    • Author(s)
      H. Oh-oka, K. Yamamoto, R. Mutoh, C. Azai, G. Kurisu
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Two disconnected antenna chlorophyll pools in type-1 photosynthetic reaction center of green sulfur bacteria2014

    • Author(s)
      C. Azai, T. Kondo, S. Itoh, H. Oh-oka
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Single-molecule spectroscopic study of photosynthetic reaction center at 6K2014

    • Author(s)
      T. Kondo1, R. Mutoh, G. Kurisu, H. Oh-oka, S. Fujiyoshi, M. Matsushita
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Structural studies on the maturation mechanism of [FeFe] hydrogenase maturation from green alga Chlamydomonas reinhardtii2014

    • Author(s)
      D. Kiyota, R. Mutoh, C. Azai, H. Oh-oka, G. Kurisu
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 緑色硫黄細菌のタイプI型RCはPS Iとは異なるエネルギー移動系をもつ2014

    • Author(s)
      浅井智広、近藤徹、伊藤繁、大岡宏造
    • Organizer
      第5回日本光合成学会年会および公開シンポジウム
    • Place of Presentation
      近畿大学農学部・奈良キャンパス(奈良)
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Research-status Report
  • [Presentation] ヘリオバクテリアI型反応中心のキノン反応・部位はPSIと異なる2014

    • Author(s)
      近藤徹、浅井智広、大岡宏造、伊藤繁
    • Organizer
      第5回日本光合成学会年会および公開シンポジウム
    • Place of Presentation
      近畿大学農学部・奈良キャンパス(奈良)
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Research-status Report
  • [Presentation] 多孔質ガラス中のナノ空間における光化学系IIコア複合体からcyt c6の間の電子伝達2014

    • Author(s)
      野地智康、鈴木孝直、近藤政晴、川上恵典、神哲郎、渡辺麻衣、池内昌彦、浅井智広、大岡宏造、南後守、伊藤繁、出羽毅久
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学・東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 緑色硫黄細菌Chlorobaculum tepidumにおいてbchX遺伝子はバクテリオクロロフィルa生合成に必須ではない2014

    • Author(s)
      浅井智広、溝口正、民秋均、大岡宏造
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 多孔性シリカ粒子に吸着したヘリオバクテリア反応中心コアタンパクの分光学的諸性質2014

    • Author(s)
      大岡宏造、野地智康、浅井智広、武藤梨沙、栗栖源嗣、伊藤繁
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 多孔質ガラス中のナノ空間における光化学系IIコア複合体からcyt c6への電子伝達2014

    • Author(s)
      野地智康、鈴木孝直、近藤政晴、川上恵典、神哲郎、渡辺麻衣、池内昌彦、浅井智広、大岡宏造、伊藤繁、出羽毅久
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学・五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 光合成反応中心タンパク質で機能する電子移動担体の極低温1分子分光2013

    • Author(s)
      近藤徹,武藤梨沙,栗栖源嗣,大岡宏造,藤芳暁,松下道雄
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 緑色硫黄細菌が持つシトクロムc-556の結晶構造解析2013

    • Author(s)
      山本和矢、浅井智広、大岡宏造、栗栖源嗣
    • Organizer
      平成25年度日本結晶学会年会
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 光合成反応中心タンパク質の極低温1分子分光2013

    • Author(s)
      近藤徹,武藤梨沙,栗栖源嗣,大岡宏造,藤芳暁,松下道雄
    • Organizer
      第7回分子科学討論会
    • Place of Presentation
      京都テレサ
    • Related Report
      2013 Research-status Report
  • [Presentation] Site-specific Structural Modification around the Special Pair of Homodimeric Photosynthetic Reaction Center in Green Sulfur Bacteria2013

    • Author(s)
      C. Azai, Y. Sano, Y. Kato, T. Noguchi and H. Oh-oka
    • Organizer
      16th International Congress of Photosynthesis
    • Place of Presentation
      St. Louis, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Heliobacterial Reaction Center Core Complex in Nanoporous Silica Material2013

    • Author(s)
      H. Oh-oka, T. Noji, C. Azai, R. Mutoh, G. Kurisu and S. Itoh
    • Organizer
      16th International Congress of Photosynthesis
    • Place of Presentation
      St. Louis, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Studies on the photosynthetic reaction centre from the green sulfur bacterium Chlorobaculum tepidum2013

    • Author(s)
      K.U. Ashraf, S. Henry, A.R. Brinth, N.J. Fraser, C. Azai, H. Oh-oka and R.J. Cogdell
    • Organizer
      16th International Congress of Photosynthesis
    • Place of Presentation
      St. Louis, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 部位特異的変異導入によるホモダイマ―型光合成反応中心の構造改変2013

    • Author(s)
      浅井 智広、佐野 裕子、加藤祐樹、野口 巧、大岡 宏造
    • Organizer
      植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 緑色硫黄細菌のホモダイマー光合成反応中心において部位特異的変異が一次電子供与体周辺の構造に与える影響2012

    • Author(s)
      浅井智広、佐野裕子、野口巧、大岡宏造
    • Organizer
      生物物理学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Research-status Report
  • [Presentation] クロロフィル色素のC17位に結合する炭化水素鎖の生合成過程2012

    • Author(s)
      原田二郎、溝口正、民秋均、大岡宏造
    • Organizer
      植物学会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 緑色硫黄細菌の光合成反応中心の部位特異的変異体:一次電子供与体周辺の水素結合構造の改変を例として2012

    • Author(s)
      浅井智広、佐野裕子、野口巧、大岡宏造
    • Organizer
      日本光合成学会
    • Place of Presentation
      東京工業大学
    • Related Report
      2012 Research-status Report
  • [Presentation] The gene expression system in the green sulfur bacterium chlorobaculum tepidum by conjugative plasmid transfer2012

    • Author(s)
      C. Azai, H. Oh-oka
    • Organizer
      国際原核光合成生物シンポジウム
    • Place of Presentation
      ポルト(ポルトガル)
    • Related Report
      2012 Research-status Report
  • [Presentation] Studies on the reduction processes from chlorophyll a estrified with geranylgeraniol to that with Δ2,6-phytadienol in the green sulfur bacterium Chlorobaculum tepidum2012

    • Author(s)
      H. Oh-oka, C. Azai, J. Harada, S. Miyago, T. Mizoguchi, H. Tamiaki
    • Organizer
      国際原核光合成生物シンポジウム
    • Place of Presentation
      ポルト(ポルトガル)
    • Related Report
      2012 Research-status Report
  • [Book] 光と生命の事典2016

    • Author(s)
      大岡宏造(共著)
    • Total Pages
      436
    • Publisher
      朝倉書店
    • Related Report
      2015 Annual Research Report
  • [Book] 光合成のエネルギー利用と環境応用2014

    • Author(s)
      三宅淳、佐々木健、橋本秀樹、民秋均、近藤政晴、南後守、樋口芳樹、杉浦美羽、浅井智広、大岡宏造、石井正治、須田彰一郎、上野嘉之、岡田行夫、三谷優、徳永博、畠岡勲、丁楠、中谷肇、堀克敏、谷生重晴、若山樹、小林肇、前田治男、藤谷泰裕ら
    • Total Pages
      308
    • Publisher
      シーエムシー出版
    • Related Report
      2014 Research-status Report
  • [Remarks] 光合成反応の分子機構

    • URL

      http://www.bio.sci.osaka-u.ac.jp/~ohoka/index.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Remarks] 光合成反応の分子機構

    • URL

      http://www.bio.sci.osaka-u.ac.jp/~ohoka/

    • Related Report
      2013 Research-status Report
  • [Remarks] 光合成反応の分子機構

    • URL

      http://www.bio.sci.osaka-u.ac.jp/~ohoka/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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