Effect of amyloid aggregation on dynamic properties of lipid membranes
| Project/Area Number |
24570185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kobe University |
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Principal Investigator |
SASAHARA KENJI 神戸大学, 医学(系)研究科(研究院), 研究員 (20432495)
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| Co-Investigator(Kenkyū-buntansha) |
MORIGAKI Kenichi 神戸大学, 遺伝子実験センター, 准教授 (10358179)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | アミロイド / 脂質膜 / モデル膜 / アミロイドβペプチド / 膵島アミロイドポリペプチド / アミロイド凝集 / モデル脂質膜 / 細胞毒性 / II型糖尿病 / 膜動態 |
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| Outline of Final Research Achievements |
We created the experimental methodology using supported planner lipid bilayers to observe the membrane binding of two peptides (islet amyloid polypeptide and amyloid beta-peptide) and their membrane-mediated amyloid aggregation. In the experimental system, the changes of membrane fluidity were quantitatively estimated for the membrane binding and subsequent aggregation of the peptides. Furthermore, it was found that the raft components of lipids were incorporated into the aggregates that deposited into the membranes in which phase separation of the raft components were involved.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Protease-dependent HA cleavage contributes to the alteration of chicken hemagglutination of the H3N2 influenza A virus2013
Author(s)
Masaoki Yamaoka, Akiko Makino, Kenji Sasahara, Aldise Mareta Nastri, Luh Ade Wilan Krisna, Edith Frederika Purhito, Emmanuel Djoko Poetranto, Laksmi Wulandari, Resti Yudhawati, Landia Setiawati, Retno Asih Setyoningrum, Kyoko Shinya
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Journal Title
Japanese Journal of Infectious Diseases
Volume: 66
Pages: 526-529
Related Report
Peer Reviewed
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