| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Aim of this study is to construct a method to report the epigenetical change using single molecule imaging. I have achieved this by measuring the diffusion property of fluorescent protein within the nucleus, and by using reporter for histone acetylation, Histac.
I found that fluorescent protein dimer change its diffusion dynamics by the differentiation status of the cell. Fluorescent protein dimer show higher diffusion in fully reprogramed iPS than in partially reprogrammed iPS. Modified Histac capable of single molecule imaging was constructed by replacing Venus with photo-activatable GFP. ES cell expressing this modified Histac increased FRET efficiency by treatment with trichostatin A, which shows that this modified Histac is capable of reporting the histone acetylation status.
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