| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
CG9281, belonging to the ATP-binding cassette family, was identified as a new clock related gene through the clock cell specific RNAi screening in Drosophila. Both the knockdown flies and the transposon insertional mutant of CG9281 showed a longer circadian period and altered mRNA levels in clock genes. Casein kinase 2 (CK2), which phosphorylates the clock proteins PER/TIM, was co-immunoprecipitated with CG9281 although the behavior analysis using CK2 mutants demonstrated that CG9281 affects circadian clock through the different pathway from CK2. Series of luciferase assays and behavior analyses imply that dominant negative mutants, which lacks the function of the second ATP-binding domain of CG9281, enhances the negative auto-feedback effect of PER/TIM, presumably through a post-transcriptional effect.
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