| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We analyzed the biochemical characteristics of human ORC, one of the proteins essential for replication initiation. We found that human ORC preferentially bound to RNA and ssDNA with G-quadruplex-formable sequences (G4-motifs). We identified the G4 motif-binding domains in the ORC1 subunit and also the amino acid residues within the domains, important for the G4-motif binding. Furthermore, we found that the G4 motif-binding activity of ORC has some specificity. It was reported that most of human replication origins have G4-motifs. We thus speculate that G4 motif-binding of ORC is involved in establishment of replication origins.
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