Functional analysis of cell cycle-associated microRNAs

• Project/Area Number: 24570201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Meiji University
• Principal Investigator: YOSHIDA Kenichi 明治大学, 農学部, 教授 (20345036)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マイクロＲＮＡ / エピジェネティクス

Outline of Final Research Achievements

MicroRNA (miRNA) has been shown to be involved in regulation of the etiology of serious illness such as cancer and metabolic diseases. It is estimated that human cells have approximately more than 1,000 kinds of miRNAs. In this study, we focused on five miRNAs that could be associated with cell cycle regulation and aimed to reveal functional aspects including target gene identification and epigenetic regulation of miRNAs. It is expected that the results obtained from this study would be useful not only for diagnosis of cancer but also for epigenetic therapy and for promotion of microRNA-based medicine.

Research Products

• [Presentation] DNA methylation analysis of miR-139 an its host gene PDE2A in human lung cancer A549 cells (2014)
  • Author(s): Fukui S, Kawakami C, Saigusa S, Yoshida K
  • Organizer: 15th IUBMB 24th FAOBMB-TSBMB International Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2014-10-21 – 2014-10-26
• [Presentation] Regulation of ERK1 and ERK2 by microRNA-483-5p in human lung cancer cell line A549 (2014)
  • Author(s): Kitagawa H, Yoshida K
  • Organizer: 15th IUBMB 24th FAOBMB-TSBMB International Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2014-10-21 – 2014-10-26
• [Presentation] ヒト肺癌細胞株A549におけるPDE2A遺伝子CpGアイランドショアのDNAメチル化とmiR-139発現の関連性 (2013)
  • Author(s): 福井櫻優里、川上知里、三枝聡、吉田健一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Book] MicroRNA as a modulator of cell proliferation and senescence: Role in lung cancer cells. 269-280 pages. In. Series. Tumor dormancy, quiescence, and senescence – Aging, cancer, and noncancer pathologies, Volume 1 edited by Hayat M.A. (2013)
  • Author(s): Fukui S, Sekiguchi M, Miyata K, Ohdaira H, Yoshida K.
  • Total Pages: 324
  • Publisher: Springer