| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Condensin complexes play an essential role in regulation of mitotic chromosome organization in eukaryotic cells. Using an experimental system to reconstitute recombinant condensin complexes in vitro, we analyzed the molecular functions of condensin I. We found that ATP binding and hydrolysis by SMC subunits have distinct contributions to condensin I’s action, and that continuous ATP hydrolysis is required for structural maintenance of chromosomes as well. We also found that balancing acts of two non-SMC subunits containing HEAT repeats support dynamic assembly of chromosome axes.
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