| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Although protein quality control is essential for maintaining cellular function, its molecular mechanism is not fully understood. In this study, we performed the characterization of the endoplasmic-reticulum (ER) deubiquitinating enzyme USP19 and showed that USP19 stabilizes the ER-associated ubiquitinating enzyme MARCH6 via deubiquitination. It is known that MARCH6 has a role in protein quality control in the ER. In addition, the stabilization of MARCH6 increases its enzymatic activity and thereby promotes degradation of the mutant ABCB11, a substrate of MARCH6. These results suggest that USP19 is involved in the regulation of protein quality control in the ER.
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