Mesoscopic analysis of CD36 clustering receptors and signal transduction in innate immune cells.

• Project/Area Number: 24570210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Showa University
• Principal Investigator: KUWATA Hirotaka 昭和大学, 歯学部, 教授 (60380523)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 免疫細胞 / 1分子イメージング / メゾスコピック解析 / 脂質リガンド / 自然免疫 / 受容体 / マクロファージ / グラム陽性菌 / スカベンジャー受容体

Outline of Final Research Achievements

As a result, we could not prepare the ligand which is labeled with fluorescent with appropriate ratio with ligand molecules. We now hypothesizes that non appropriate ratio labeling have caused the inaccessibility of ligand to receptors. We have tried to get the suitable way to label the receptor with the different approach using monoclonal antibody which are now available as a commercial product.

Research Products

• [Journal Article] Role of protease maturation lipoprotein in osmoadaptation of Streptococcus mutans. (2014)
  • Author(s): Kunii M, Arimoto T, Hasegawa T, Kuwata H, Igarashi T.
  • Journal Title: FEMS Microbiol Lett. Volume: 356(1) Issue: 1 Pages: 45-52
  • DOI: 10.1111/1574-6968.12477
  • Peer Reviewed / Open Access
• [Journal Article] Hydrogen peroxide contributes to the epithelial cell death induced by the oral mitis group of streptococci. (2014)
  • Author(s): Okahashi N, Sumitomo T, Nakata M, Sakurai A, Kuwata H, and Kawabata S.
  • Journal Title: PLoS One Volume: 9 Issue: 1 Pages: 88136-88136
  • DOI: 10.1371/journal.pone.0088136
  • Peer Reviewed / Open Access
• [Journal Article] Rothia dentocariosa induces TNF-alpha production in a TLR2-dependent manner. (2014)
  • Author(s): Kataoka H, Taniguchi M, Fukamachi H, Arimoto T, Morisaki H, Kuwata H.
  • Journal Title: Pathog Dis. Volume: 71(1) Issue: 1 Pages: 65-8
  • DOI: 10.1111/2049-632x.12115
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial sphingophospholipids containing non-hydroxy fatty acid activate murine macrophages via Toll-like receptor 4 and stimulate bacterial clearance (2013)
  • Author(s): Fujiwara, N.
  • Journal Title: Biochim. Biophys. Acta. Volume: Mar 29 Issue: 6 Pages: 1177-84
  • DOI: 10.1016/j.bbalip.2013.03.008
  • Peer Reviewed
• [Journal Article] Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function in human primary macrophage (2012)
  • Author(s): Kuwata H.
  • Journal Title: Seikagaku Volume: 84(12) Pages: 1012-1016
  • NAID: 10031145997