Genome wide search for target genes of Drosophila histone methyl transferase dG9a
Project/Area Number |
24570211
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hideki 京都工芸繊維大学, 大学院工芸科学研究科, 助教 (30570600)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 発生・分化 / 遺伝学 / 昆虫 / ヒストンメチル化酵素 / EGFR経路 / 形態形成 / オートファジー / RNA-seq / 遺伝子 / エピゲノム |
Outline of Final Research Achievements |
We have identified segmentation genes that are upregulated in embryos of Drosophila G9a (dG9a) mutant. Since temporal and spatial expression pattern of these segmentation genes are not changed in dG9a mutant embryos in compared with the wild type, delay in embryogenesis of the dG9a mutant is likely caused by the changes in expression level of their mRNAs. Genome wide genetic screen by using the rough eye phenotype induced by eye disc specific dG9a-overexpression identified a set of genes involved in the EGFR signaling pathway and autophagy. In consistent with these observations, the dG9a mutant showed high sensitivity to starvation stress that induce autophagy. These findings provide important clues to clarify epigenetic regulation of autophagy.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Drosophila myeloid leukemia factor acts with DREF to activate the JNK signaling pathway.2014
Author(s)
Yanai, H., Yoshioka, Y., Yoshida, H., Nakao, Y., Plessis, A. and Yamaguchi, M.
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Journal Title
Oncogenesis
Volume: 3
Pages: e98
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] DNA polymerase alpha interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila.2014
Author(s)
Sahashi, R., Crevel G., Pasko, J., Suyari, O., Nagai, R., Saura, M. M., Yamaguchi, M. and Cotterill, S.
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Journal Title
J. Cell Sci.
Volume: 127
Pages: 3066-3078
DOI
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Genetic link between Cabeza, a Drosophila homologue of Fused in Sarcoma (FUS), and the EGFR signaling pathway.2014
Author(s)
Shimamura, M., Kyotani, A., Azuma, Y., Yoshida, H., Nguyen, T. B., Mizuta, I., Yoshida, T., Mizuno, T., Nakagawa, M., Tokuda, T. and Yamaguchi, M.
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Journal Title
Exp. Cell. Res.
Volume: 326
Pages: 36-45
DOI
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Role of SCOX in determination of Drosophila melanogaster lifespan.2014
Author(s)
Nguyen, T. B., Ida, H., Shimamura, M., Kitazawa, D., Akao, S., Yoshida, H., Inoue, Y. H. and Yamaguchi, M.
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Journal Title
American J. Cancer Res.
Volume: 4(4)
Pages: 325-336
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Drosophila DOCK family protein Sponge regulates the JNK pathway during thorax development.2014
Author(s)
Morishita, K., Ozasa, F., Eguchi, K., Yoshioka, Y., Yoshida, H., Hiai, H. and Yamaguchi, M.
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Journal Title
Cell Struct. Funct.
Volume: 39
Pages: 113-124
DOI
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Drosophila MCM10 is required for DNA replication and differentiation in the compound eye.2014
Author(s)
Vo, N., Taga, A., Inaba, Y., Hideki, Y., Cotterill, S. and Yamaguchi, M.
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Journal Title
PLoS ONE
Volume: 9(3)
Pages: e93450
DOI
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Peer Reviewed
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[Journal Article] Identification of ter94, Drosophila VCP, as a strong modulator of motor neuron degeneration induced by knockdown of Caz, Drosophila FUS.2014
Author(s)
Azuma, Y., Tokuda, T., Shimamura M., Kyotani, A., Sasayama H., Yoshida, T., Mizuta, I., Mizuno, T., Nakagawa, M., Fujikake, N., Ueyama, M., Nagai, Y. and Yamaguchi, M.
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Journal Title
Hum. Mol. Genet.
Volume: 23
Pages: 1-14
DOI
Related Report
Peer Reviewed
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[Journal Article] DNA polymerase alpha interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila.2014
Author(s)
Sahashi, R., Crevel G., Pasko, J., Suyari, O., Nagai, R., Saura, M. M., Yamaguchi, M. and Cotterill, S.
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Journal Title
J. Cell Science
Volume: 未定
Pages: 未定
Related Report
Peer Reviewed
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[Journal Article] dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development.2013
Author(s)
Ly, L.L., Suyari, O., Yoshioka, Y., Tue, N.T., Yoshida, H. and Yamaguchi, M.
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Journal Title
Gene
Volume: 520
Pages: 106-118
DOI
Related Report
Peer Reviewed
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[Journal Article] The Drosophila DOCK family protein Sponge is involved in differentiation of R7 photoreceptor cells.2013
Author(s)
Eguchi, K., Yoshioka, Y., Yoshida, H., Morishita, K., Miyata, S., Hiai, H. and Yamaguchi, M.
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Journal Title
Exp. Cell Res.
Volume: 315 (14)
Pages: 2179-2195
DOI
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Peer Reviewed
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[Journal Article] Genetic link between Heme Oxygenase and the signaling pathway of DNA damage in Drosophila melanogaster.2013
Author(s)
Ida, H., Suyari, O., Shimamura, M., Tran, T. T., Yamaguchi, M. and Taketani, S.
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Journal Title
Tohoku J. Exp. Med.
Volume: 231
Pages: 117-125
DOI
Related Report
Peer Reviewed
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[Journal Article] Functional analysis of Drosophila DNA polymerase ε p58 subunit.2013
Author(s)
Sahashi, R., Matsuda, R., Suyari, O., Kawai, M., Yoshida, H., Cotterill, S. and Yamaguchi, M.
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Journal Title
Am. J. Cancer Res.
Volume: 3(5)
Pages: 478-489
DOI
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Peer Reviewed
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[Journal Article] Knockdown of the Drosophila Fused in Sarcoma (FUS) homologue causes deficient locomotive behavior and shortening of motoneuron terminal branches.2012
Author(s)
Sasayama, H., Shimamura, M., Tokuda, T., Azuma, Y., Yoshida, T., Mizuno, T., Nakagawa, M., Fujikake, N., Nagai, Y. and Yamaguchi, M.
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Journal Title
PLoS ONE
Volume: 7
Pages: e39483
DOI
Related Report
Peer Reviewed
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[Journal Article] Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle.2012
Author(s)
Suyari, O., Kawai, M., Ida, H., Yoshida, H., Sakaguchi, K. and Yamaguchi, M.
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Journal Title
Gene
Volume: 495
Pages: 104-114
DOI
Related Report
Peer Reviewed
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[Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis.2012
Author(s)
Ushijima Y., Inoue, Y. H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H. and Yamaguchi, M.
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Journal Title
Chromosome Res.
Volume: 20
Pages: 319-331
DOI
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Peer Reviewed
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[Presentation] A genome-wide screen for mRNAs targeting the ER in SRP-independent manner, and analysis on subcellular localization of yki mRNA2014
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tesuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Hideki Yoshida
Organizer
第37回 日本分子生物学会年会
Place of Presentation
パシフィコ横浜(横浜市)
Year and Date
2014-11-25 – 2014-11-27
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[Presentation] Identification of non-canonical mRNAs targeting to the ER and analysis on subcellular localization of mRNAs by RNA imaging system2014
Author(s)
Takanari Umegawachi, Hiromu Koshida, Momoko Yamada, Kazuma Usui, Tesuya Sato, Mikita Suyama, Megumi Ito, Yasuyuki Ohkawa, Masamitsu Yamaguchi, Henry M. Krause, Hideki Yoshida
Organizer
The Nineteenth Annual Meeting of the RNA Society
Place of Presentation
Quebec city convention centre(カナダ・ケベックシティ)
Year and Date
2014-06-03 – 2014-06-08
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