The chromatin signaling regulates the formation of cell polarity.

• Project/Area Number: 24570213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: HIEDA Miki 大阪大学, 医学(系)研究科(研究院), 助教 (00380254)
• Co-Investigator(Renkei-kenkyūsha): KURONO Sadamu (20271554)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 核膜 / 細胞極性 / 乳癌 / LINC complex / 癌転移 / SUN1 / nesprin / SUN / ヒストンメチル化 / ヒストン / 細胞運動 / 乳がん / ヒストン修飾 / 癌細胞

Outline of Final Research Achievements

Formation of cell polarity plays fundamental roles in cell migration, cell differentiation, and carcinogenesis etc. The mammalian Golgi appratus has important function in the formation of cell polarity. In this study, we showed that histone methylation enzyme is required for proper Golgi structure via LINC (linker of nucleoskeleton and cytoskeleton) complex, a nuclear envelope protein complex consisting mainly of the SUN and nesprin protein families. In addition, we demonstrated global loss of a nuclear lamina component, lamin A/C and LINC complex components SUN1, SUN2, and nesprin-2 in breast cancer.

Research Products

• [Journal Article] SUV420H2 suppresses breast cancer cell invasion through down regulation of the SH2 domain-containing focal adhesion protein tensin-3. (2015)
  • Author(s): Shinchi, Y., Hieda, M.*, Nishioka, Y., Matsumoto, A., Yokoyama, Y., Kimura, H., Matsuura, S., Matsuura, N.
  • Journal Title: Experimental Cell Research, Volume: in press Issue: 1 Pages: 90-99
  • DOI: 10.1016/j.yexcr.2015.03.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Histone modifications associated with cancer cell migration and invasion. (2015)
  • Author(s): Hieda, M., Matsuura, N., and Kimura, H.
  • Journal Title: Methods in Molecular Biology Volume: 1238 Pages: 301-317
  • DOI: 10.1007/978-1-4939-1804-1_16
  • ISBN: 9781493918034, 9781493918041
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Loss of histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with invasive activity. (2014)
  • Author(s): Yokoyama Y, Matsumoto A, Hieda M*, Shinchi Y, Ogihara E, Hamada M, Nishioka Y, Kimura H, Yoshidome K, Tsujimoto M, Matsuura N.
  • Journal Title: Breast Cancer Res. Volume: 16 Issue: 3
  • DOI: 10.1186/bcr3681
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cancer associated up-regulation of H3K9 methylation promotes cell motility in vitro and drives tumor formation in vivo. (2013)
  • Author(s): Yokoyama, Y., Hieda, M., Nishioka, Y., Matsumoto, A., Higashi, S., Kimura, H., Yamamoto, H., Mori, M., Matsuura, S., and Matsuura, N.
  • Journal Title: Cancer Science Volume: 104 Issue: 7 Pages: 889-895
  • DOI: 10.1111/cas.12166
  • Peer Reviewed
• [Journal Article] The cytoplasmic tail of Heparin-binding EGF like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER. (2012)
  • Author(s): Hieda, M., Koizumi, M., Higashi, C., Tachibana, T., Taguchi, T., and Higashiyama, S.
  • Journal Title: FEBS Open Bio. Volume: 4 Pages: 721-726
  • Peer Reviewed
• [Presentation] ヒストン修飾酵素SUV39H1は核膜タンパク質SUN1/SUN2の機能を介してゴルジ体構築を制御する。 (2014)
  • Author(s): 檜枝美紀、西岡 優、木村宏、松浦成昭
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都国際会館、京都府京都市
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] SUV420H2 expression suppresses breast cancer cell invasion through expression of the SH2 domain-containing focal adhesion protein tensin-3 (2014)
  • Author(s): Yoshimi Shinchi, Ayaka Matsumoto, Yuhki Yokoyama, Nariki Matsuura, and Miki Hieda
  • Organizer: 熊本医学・生物科学国際シンポジウム 「幹細胞制御と臓器再建」
  • Place of Presentation: 熊本市医師会館、熊本県熊本市
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] Cancer-associated aberrant histone modification promotes cell motility and invasion in several ways. (2014)
  • Author(s): Hieda M.
  • Organizer: Seventh international Epigenomics & Metabolomics, 2014 meeting,
  • Place of Presentation: The Courtyard Marriott, Boston, MA, USA
  • Year and Date: 2014-08-25 – 2014-08-26
  • Invited
• [Presentation] Down-regulation of SUN-KASH nuclear envelope bridges in breast cancer (2014)
  • Author(s): Matsumoto Ayaka, Hieda Miki, Matsuura Nariaki,
  • Organizer: The 2nd International Symposium of Training Plan for Oncology Professionals
  • Place of Presentation: 大阪府大阪市
  • Invited
• [Presentation] Aberrant histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with cancer cell invasion. (2013)
  • Author(s): Miki Hieda, Ayaka Matsumoto, Yuhki Yokoyama, Yu Nishioka, Yoshimi Shinchi, Nariaki Matsuura
  • Organizer: Annual Meeting for the American Society for Cell Biology
  • Place of Presentation: New Oreans, Louisiana, USA
• [Presentation] ヒストンメチル化酵素SUV420H2の発現は乳癌細胞浸潤能を抑制する (2013)
  • Author(s): 横山雄起、檜枝美紀、新地純美、松本彩花、西岡優、松浦成昭
  • Organizer: 第31回染色体ワークショップ・第11回核ダイナミクス研究会
  • Place of Presentation: 神奈川県足柄下郡箱根町
• [Presentation] ヒストンメチル化酵素SUV420H2の発現は乳癌細胞浸潤能を抑制する (2013)
  • Author(s): 横山雄起、檜枝美紀 、松本彩花 、新地純美、西岡優、木村宏、吉留克英、辻本正彦、松浦成昭
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Cancer associated up-regulation of H3K9 methylation promotes cell motility in vitro and drives tumor formation in vivo (2013)
  • Author(s): 檜枝美紀、松浦成昭、
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] 細胞膜と核膜のコミュニケーション (2013)
  • Author(s): 檜枝美紀
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 神奈川県横浜市
  • Invited
• [Presentation] 細胞膜と細胞核のコミュニケーション (2013)
  • Author(s): 檜枝美紀
  • Organizer: 第86回生化学会大会 (シンポジウム）
  • Place of Presentation: パシフィコ横浜 (神奈川）
  • Invited
• [Presentation] H3 lysine 9 methylation promotes cell motility in vitro and drives tumor formation in vivo. (2013)
  • Author(s): Yokoyama, Y., Hieda, M., Higashi, S., Nishioka, Y., Matsumoto, A., Kimura, H., Matsuura, S., Matsuura N.,
  • Organizer: Keystone Symposia, Epigenetic Marks and Cancer Drugs (C8)
  • Place of Presentation: Santa Fe, NM, USA
• [Presentation] H3 lysine 9 methylation promotes cell motility in vitro and drives tumor formation in vivo; a possible explanation for the cancer-associated. (2012)
  • Author(s): Hieda, M., Nishioka, Y., Yokoyama, Y., Matsuura, N., Matsuura, S.,
  • Organizer: Annual Meeting for the American Society for Cell Biology
  • Place of Presentation: San Francisco, CA, USA
• [Presentation] Histone H3 lysine 9 methylation promotes cell motility in vitro and drives tumor formation in vivo (2012)
  • Author(s): 檜枝美紀、横山雄起、西岡優、木村宏、松浦脩治、松浦成昭
  • Organizer: 第85回日本生化学会大会 (シンポジウム）
  • Place of Presentation: 福岡国際会議場 (福岡)
  • Invited
• [Presentation] ヒストンH4K20メチル化酵素 SUV420H2の過剰発現は細胞浸潤能を抑制する (2012)
  • Author(s): 新地純美、西岡優、横山雄起、檜枝美紀、松浦脩治、松浦成昭
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場 (福岡)
• [Presentation] Histone H3 lysine 9 methylation promotes cell motility in vitro and drives tumor formation in vivo (2012)
  • Author(s): Hieda, M., Yokoyama, Y., Nishioka, Y., Kimura, H., Matsuura, S., Matsuura, S.
  • Organizer: Cold Spring Harbor Laboratory meeting, Epigenetics & Chromatin
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Chromatin signaling relays to the Golgi structure via SUN/KASH protein (2012)
  • Author(s): Hieda, M., Nishioka, Y., Yokoyama, Y., Matsuura, N., Matsuura, S.
  • Organizer: 第45回日本発生生物学会/第64回細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場 (兵庫)
• [Book] Heparin: Structure, Drug Delivery Applications and Clinical Outcomes (2014)
  • Author(s): Miki Hieda
  • Total Pages: 102
  • Publisher: Nova Science Publishers, Inc