| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The methylotrophic yeast Hansenula polymorpha was established as a model system. Towards this end, I first investigated the previously uncharacterized mating type determination system and the mechanism of self-fertility, and revealed a novel mating type switching system where only one of two mating type loci is transcriptionally active and the chromosomal inversion of their intervening region causes the switching of the mating type identity. This chromosomal inversion-based mechanism represents a novel form of mating type switching that requires only two MAT loci.
I also investigated the regulation of nuclear segregation. In H. polymorpha, unlike in other budding yeast, the nuclear position is not tightly regulated in early stages of the cell cycle, which is due to the poor function of cytoplasmic microtubules. Microscopic analyses of spindle pole bodies (SPB) suggested the structure and function of the cytoplasmic side of SPB is cell cycle regulated.
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