| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Dynamin is an important protein to cut the vesicles from the cell membrane during endocytosis. Recently, dynamin has shown to play an important role on cytokinesis, which is a final stage of cell division. Inhibitors against dynamin, which can be used for targeting cytokinesis of cancer cells, are new potent and attractive anti-cancer reagents. This study clarified the function and dynamics of dynamin in cytokinesis of cellular slime mold cells, a model organism for cytokinesis. Dynamin null cells showed severe defects in cytokinesis, resulting in their multinucleation. Actin and myosin II could not recruit at the cleavage furrow properly. GFP-tagged dynamins accumulated to the cleavage furrow. We found that dynamins contribute to stabilization of actin in the contractile ring. Together, we proposed a new model for the role of dynamin in cytokinesis.
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