Characterization of an arresting-like protein involved in the internalization of syntaxin during meiosis
| Project/Area Number |
24570219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Osaka City University |
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Principal Investigator |
Nakamura Taro 大阪市立大学, 大学院理学研究科, 教授 (30291082)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 膜輸送 / シンタキシン / アレスチン / 酵母 / 胞子形成 / エンドサイトーシス |
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| Outline of Final Research Achievements |
We conducted live imaging of endocytosis during meiosis by fluorescence microscopy and demonstrated that Mug170 localizes to the specific region on the plasma membrane of zygote. We also observed dynamic behavior of Psy1 during meiosis and determined the lysine residue required for its internalization. No HECT-type ubiquiin ligase mutants showed defects in internalization of Psy1. Moreover, we identified 20 genes involved in Psy1 internalization, which encode ubiquitin ligase, cyclin, and microtubule-related proteins.
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Report
(5 results)
Research Products
(30 results)
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[Presentation] Structure of the spore surface.2015
Author(s)
Kana Fukunishi, Kana Miyakubi, Aiko Hirata, Chikashi Shimoda, Taro Nakamura
Organizer
THE EIGHTH INTERNATIONAL FISSION YEAST MEETING
Place of Presentation
Ikuta Shrine, Kobe, Japan
Year and Date
2015-06-21
Related Report
Int'l Joint Research / Invited
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