| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
At the one set of cell division of metazoan, the nuclear envelope (NE) breaks down and the nuclear membrane components are absorbed to the ER. After chromosome segregation, NE is reassembled around the daughter chromosomes. Reassembly of the NE is crucial to organize a functional nucleus and to ensure proper progression of cell cycle. I found that NE containing subdomains characterized by LBR and emerin can be reconstituted by supplying mitotic cytosol and CDK inhibitor to semi-intact mitotic cells. The reconstituted NE is competent for active nuclear transport, showing the integrity of NE. To localization LBR and emerin to chromosome from the mitotic ER, at least two factors are needed. The results of inhibitor effects and immunostaining analysis indicated that the accumulation of LBR-membranes is positively regulated by PP1. It is shown that this newly developed system allows us to elucidate mitotic stage specific regulatory factor(s) for NE formation.
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