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Identification of nuclear pore complex proteins involved in nuclear differentiation of bi-nucleated unicellular organism Tetrahymena

Research Project

Project/Area Number 24570227
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

IWAMOTO MASAAKI  独立行政法人情報通信研究機構, 未来ICT研究所バイオICT研究室, 主任研究員 (80450683)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords核膜孔複合体 / ヌクレオポリン / 2核性 / 繊毛虫 / テトラヒメナ / 核分化 / マススペクトロメトリー
Outline of Final Research Achievements

In this study, nuclear pore complex proteins of the binucleated unicellular organism Tetrahymena were comprehensively identified. Hereby, it became clear how some of the nuclear pore complex components were different between macronucleus and micronucleus. I studied the molecular functions of some components involved in generating of the structural difference between them, and successfully found probable components that had a major role in nuclear differentiation. This finding provides a new insight into understanding the mechanism that causes nuclear differentiation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (23 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Invited: 4 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena2015

    • Author(s)
      Masaaki Iwamoto, Takako Koujin, Hiroko Osakada, Chie Mori, Tomoko Kojidani, Atsushi Matsuda, Haruhiko Asakawa, Yasushi Hiraoka, Tokuko Haraguchi
    • Journal Title

      Journal of Cell Science

      Volume: 128 Pages: 1812-1823

    • DOI

      10.1242/jcs.167353

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe2014

    • Author(s)
      Asakawa H, Yang HJ, Yamamoto T G, Ohtsuki C, Chikashige Y, Sakata-Sogawa K, Tokunaga M, Iwamoto M, Hiraoka Y, Haraguchi T.
    • Journal Title

      Nucleus

      Volume: 5(印刷中) Issue: 2 Pages: 1-14

    • DOI

      10.4161/nucl.28487

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena2014

    • Author(s)
      Masaaki Iwamoto, Chie Mori, Yasushi Hiraoka, Tokuko Haraguchi
    • Journal Title

      Gene

      Volume: 534 Issue: 2 Pages: 249-255

    • DOI

      10.1016/j.gene.2013.10.049

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Monoclonal antibodies recognize Gly-Leu-Phe-Gly repeat of nucleoporin Nup98 of Tetrahymena, yeasts and humans2013

    • Author(s)
      Masaaki Iwamoto et al.
    • Journal Title

      Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

      Volume: 32 Issue: 2 Pages: 81-90

    • DOI

      10.1089/mab.2012.0118

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 二核性繊毛虫テトラヒメナの核膜孔複合体と核-細胞質間輸送2015

    • Author(s)
      岩本政明
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学三鷹キャンパス(東京都三鷹市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 繊毛虫の大核と小核を分ける核膜孔複合体の構造と機能2014

    • Author(s)
      岩本政明、荒神尚子、小坂田裕子、森知栄、平岡泰、原口徳子
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] テトラヒメナのヒストン修飾の観察2014

    • Author(s)
      丸山顕史、岩本政明、平岡泰、原口徳子
    • Organizer
      第47回日本原生生物学会大会
    • Place of Presentation
      宮城教育大学(仙台市)
    • Year and Date
      2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 繊毛虫テトラヒメナの核膜孔複合体:その構造の進化的な共通性と特殊性2014

    • Author(s)
      岩本政明、小坂田裕子、森知栄、福田康弘、長尾恒治、小布施力史、平岡泰、原口徳子
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Year and Date
      2014-06-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分裂酵母における核膜孔複合体の構成と機能2014

    • Author(s)
      浅川東彦、Yang Hui-Ju、山本孝治、大槻千鶴、近重裕次、十川久美子、徳永万喜洋、岩本政明、平岡泰、原口徳子
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Year and Date
      2014-06-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大小核をもつテトラヒメナの核膜孔複合体構成因子の網羅的解析2013

    • Author(s)
      岩本政明、小坂田裕子、森知栄、長尾恒治、小布施力史、平岡泰、原口徳子
    • Organizer
      第31回染色体ワークショップ・第12回核ダイナミクス研究会
    • Place of Presentation
      ホテルおかだ、神奈川県
    • Related Report
      2013 Research-status Report
  • [Presentation] Comprehensive characterization of nucleoporins reveals structural difference between MAC and MIC nuclear pore complexes of Tetrahymena thermophila

    • Author(s)
      Masaaki Iwamoto, Hiroko Osakada, Chie Mori, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi
    • Organizer
      FASEB Science Research Conferences, Ciliate Molecular Biology
    • Place of Presentation
      Steamboat Springs, Colorado, USA
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ライブクレム法:分子ダイナミクスを高分解能で観察する方法

    • Author(s)
      原口徳子、他
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば国際会議場、茨城県
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Biased assembly of the nuclear pore complex determines nuclear differentiation in the ciliate Tetrahymena thermophila

    • Author(s)
      岩本政明、他
    • Organizer
      第45回発生生物学会・第64回細胞生物学会合同大会
    • Place of Presentation
      神戸国際会議場・神戸商工会議所、兵庫県
    • Related Report
      2012 Research-status Report
  • [Presentation] Identification of nuclear localization signals to distinguish macronucleus and micronucleus in bi-nucleated Tetrahymena thermophila

    • Author(s)
      原口徳子、他
    • Organizer
      第45回発生生物学会・第64回細胞生物学会合同大会
    • Place of Presentation
      神戸国際会議場・神戸商工会議所、兵庫県
    • Related Report
      2012 Research-status Report
  • [Presentation] The nuclear pore complex determines nuclear differentiation in ciliate Tetrahymena thermophila

    • Author(s)
      Tokuko Haraguchi et al.
    • Organizer
      Cold Spring Harbor Laboratory Meeting, Dynamic Organization of Functional Nucleus
    • Place of Presentation
      Cold Spring Harbor Laboratory, New York, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] テトラヒメナにおける核膜孔複合体タンパク質の網羅的探索

    • Author(s)
      岩本政明、他
    • Organizer
      第45回日本原生動物学会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス、兵庫県
    • Related Report
      2012 Research-status Report
  • [Presentation] 核膜孔複合体が二核性生物テトラヒメナの核分化を決定する

    • Author(s)
      岩本政明、他
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡、福岡県
    • Related Report
      2012 Research-status Report
  • [Presentation] テトラヒメナの大小核の核膜孔複合体を構成するヌクレオポリンタンパク質の網羅的探索

    • Author(s)
      岩本政明、他
    • Organizer
      第30回染色体ワークショップ・第11回核ダイナミクス研究会
    • Place of Presentation
      兵庫県立淡路夢舞台国際会議場、兵庫県
    • Related Report
      2012 Research-status Report
  • [Book] 染色体と細胞核のダイナミクス、第9章 核膜の機能・構造・ダイナミクス2013

    • Author(s)
      原口徳子、岩本政明
    • Total Pages
      224
    • Publisher
      (株)化学同人
    • Related Report
      2013 Research-status Report
  • [Remarks] Cell Magic World 生物情報グループ

    • URL

      http://www2.nict.go.jp/advanced_ict/bio/w131103/CellMagic/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] Cell Magic World 生物情報グループ

    • URL

      http://www2.nict.go.jp/advanced_ict/bio/w131103/CellMagic/index.html

    • Related Report
      2013 Research-status Report
  • [Remarks] Cell Magic World 生物情報グループ

    • URL

      http://www2.nict.go.jp/advanced_ict/bio/w131103/CellMagic/index.html

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 核膜孔タンパク質Nup98を特異的に認識するモノクローナル抗体2013

    • Inventor(s)
      原口徳子、岩本政明
    • Industrial Property Rights Holder
      独立行政法人情報通信研究機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-061612
    • Filing Date
      2013-03-25
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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