Study of cell interacting mechanism during fish tissue regeneration

• Project/Area Number: 24570233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Kawakami Atsushi 東京工業大学, 生命理工学研究科, 准教授 (00221896)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ゼブラフィッシュ / 再生 / 細胞系譜 / トランスジェニック / Fgfシグナル / Fgf / Wnt / 小型魚類 / Wntシグナル

Outline of Final Research Achievements

To understand the molecular processes of tissue regeneration, we aimed to elucidate the signalling mechanism and cellular functions that control the zebrafish fin regeneration. We analyzed the role of Fgf signals during regeneration and revealed the essential functions of Fgf20a and Fgf3. Further, we analyzed a mutant that displays the apoptosis of regenerating cells and showed that a diffusible substance from the myeloid cells supports the survival and proliferation of regenerating cells.

Research Products

• [Journal Article] A diffusible signal derived from hematopoietic cells supports the survival and proliferation of regenerative cells during zebrafish fin fold regeneration (2015)
  • Author(s): T. Hasegawa, T. Nakajima, T. Ishida, A. Kudo and A. Kawakami
  • Journal Title: Dev Biol Volume: 399 Issue: 1 Pages: 80-90
  • DOI: 10.1016/j.ydbio.2014.12.015
  • Peer Reviewed / Open Access
• [Journal Article] Colored medaka and zebrafish: Transgenics with ubiquitous and strong transgene expression driven by the medaka b-actin promoter. (2012)
  • Author(s): Yoshinari, N., Ando, K., Kudo, A., Kinoshita, M.and Kawakami, A.
  • Journal Title: Development Growth & Differentiation Volume: 54 Issue: 9 Pages: 818-828
  • DOI: 10.1111/dgd.12013
  • Peer Reviewed
• [Presentation] Zebrafish fin fold regeneration requires proper control of inflammation via macrophage. (2016)
  • Author(s): Tomoya Hasegawa, Christopher J.Hall, Philip S. Crosier, Akira Kudo, and Atsushi Kawakami
  • Organizer: International Conference on Zebrafish Development and Genetics
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2016-07-13
  • Int'l Joint Research
• [Presentation] Committed stem cells derived from the somites supply the osteoblasts during adult bone homeostasis and regeneration (2016)
  • Author(s): Kazunori Ando, Eri Shibata, Akira Kudo, Gembu Abe, Koichi Kawakami, Stefan Hans, Michael Brand, Atsushi Kawakami
  • Organizer: International Conference on Zebrafish Development and Genetics
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2016-07-13
  • Int'l Joint Research
• [Presentation] Zebrafish fin fold regeneration requires proper control of inflammation via macrophage. (2016)
  • Author(s): Tomoya Hasegawa, Christopher J.Hall, Philip S. Crosier, Akira Kudo, and Atsushi Kawakami
  • Organizer: 日本発生生物学会大会
  • Place of Presentation: 熊本
  • Year and Date: 2016-05-31
• [Presentation] Cell fates of wound epidermis during zebrafish fin regeneration (2015)
  • Author(s): Eri Shibata, Akira Kudo, Atsushi Kawakami
  • Organizer: European Zebrafish Meeting
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] Chronic inflammation induces the blastema apoptosis during zebrafish fin fold regeneration (2015)
  • Author(s): Tomoya Hasegawa, Akira Kudo, and Atsushi Kawakami
  • Organizer: 日本発生生物学会大会
  • Place of Presentation: 筑波
  • Year and Date: 2015-06-02
• [Presentation] Distal blastema is a signaling center that directs cell proliferation during zebrafish fin regeneration (2014)
  • Author(s): Yuki Yokota, Natsumi Horita, Gembu Abe, Koichi Kawakami, Akira Kudo, and Atsushi Kawakami
  • Organizer: 第37回日本分子生物学会大会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Wound epidermal signal as a director of zebrafish fin regeneration (2014)
  • Author(s): Eri Shibata, Yuki Yokota, Natsumi Horita, Gembu Abe, Koichi Kawakami, Akira Kudo and Atsushi Kawakami
  • Organizer: 第37回日本分子生物学会大会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] A trophic factor derived from the myeloid cells supports the survival and proliferation of blastema during tissue regeneration in zebrafish (2014)
  • Author(s): Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Akira Kudo, Atsushi Kawakami
  • Organizer: 第47回日本発生生物学会大会
  • Place of Presentation: ウインク愛知（名古屋）
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Transgenic imaging and tracing of the wound epidermis during zebrafish fin regeneration (2014)
  • Author(s): Eri Shibata, Kazunori Ando, Natsumi Horita, Gembu Abe, Koichi Kawakami, Akira Kudo, Atsushi Kawakami
  • Organizer: 第47回日本発生生物学会大会
  • Place of Presentation: ウインク愛知（名古屋）
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Distal blastema is a signaling center that directs cell proliferation during zebrafish fin regeneration (2014)
  • Author(s): Yuki Yokota, Natsumi Horita, Gembu Abe, Koichi Kawakami, Akira Kudo, and Atsushi Kawakami
  • Organizer: 第47回日本発生生物学会大会
  • Place of Presentation: ウインク愛知（名古屋）
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] Imaging and characterization of mmp9-expressing cells during zebrafish fin regeneration using the Tol2 BAC transgenic (2013)
  • Author(s): Kazunori Ando, Akira Kudo, Atsushi Kawakami
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: くにびきメッセ（島根県）
• [Presentation] Critical role of fibronectin 1b during zebrafish fin regeneration (2013)
  • Author(s): Eri Shibata, Emiko Murase, Akira Kudo and Atushi Kawakami
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: くにびきメッセ（島根県）
• [Presentation] Analysis of the source of ‘blastema survival factor’ and its downstream signaling during the zebrafish fin fold regeneration (2013)
  • Author(s): Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Akira Kudo, Atsushi Kawakami
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: くにびきメッセ（島根県）
• [Presentation] Blastema survival and proliferation are supported by a factor derived from hematopoietic/endothelial tissues during zebrafish fin fold regeneration (2013)
  • Author(s): Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Akira Kudo, Atsushi Kawakami
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: The Palau de Congressos de Catalunya
• [Presentation] An intercalation model in the zebrafish fin regeneration (2013)
  • Author(s): Yuki Yokota, Akira Kudo, and Atsushi Kawakami
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報産業プラザ
• [Presentation] Identification of bone stem cells during growth and regeneration of endo- and exo-skeletons in zebrafish (2013)
  • Author(s): Kazunori Ando, Genbu Abe, Akira Kudo, Koichi Kawakami, and Atsushi Kawakami
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報産業プラザ
• [Presentation] Differential actions of Fgf signaling controls blastema formation and regeneration in zebrafish (2013)
  • Author(s): Differential actions of Fgf signaling controls blastema formation and regeneration in zebrafish
  • Organizer: 第19回 小型魚類研究会
  • Place of Presentation: 仙台市情報産業プラザ
• [Presentation] Establishment of nitroreductase-mediated cell ablation using Cre-loxP system in zebrafish (2012)
  • Author(s): Kazunori Ando, Nozomi Yoshinari, Atsushi Kawakami
  • Organizer: 第45回日本発生生物学会大会
  • Place of Presentation: 神戸国際会議場〔兵庫県）
• [Presentation] Extrinsic factor controls blastema proliferation and survival during fin fold regeneration (2012)
  • Author(s): Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Atsushi Kawakami
  • Organizer: 第45回日本発生生物学会大会
  • Place of Presentation: 神戸国際会議場〔兵庫県）
• [Presentation] Blastema survival depends on a diffusible factor that acts from a distance during fin fold regeneration (2012)
  • Author(s): Tomoya Hasegawa, Teruhiro Nakajima, Takashi Ishida, Akira Kudo and Atsushi Kawakami
  • Organizer: 第12回 小型魚類研究会
  • Place of Presentation: 芝蘭会館〔京都府）