Mechanism for rapid wound healing and initiation of regeneration

• Project/Area Number: 24570239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Hyogo
• Principal Investigator: MOCHII Makoto 兵庫県立大学, 生命理学研究科, 准教授 (90202358)
• Research Collaborator: OOSIGI Tadahiro ; YASUKAWA Shigehiro ; OKUMURA Akinari ; SATO Kentaro ; HARADA Seiya
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 尾部再生 / 傷修復 / 脊索 / 再生開始 / シグナル / アフリカツメガエル / 再生 / xles1 / 傷表皮

Outline of Final Research Achievements

I analyzed wound healing and regeneration initiation after tail amputation using Xenopus tadpoles.
1. Analysis of notochord fragments grafted under host skin suggested that wounding of notochord, skin and muscle tissue is necessary to initiate notochord regeneration.
2. I established transgenic lines harboring egfp under control of xes1 promoter/enhancer, in which wound epidermis is labeled in situ. Pharmacological analysis using the transgenic tadpoles showed that TGF-β, ROS and ERK signals are required for both es1 expression and proper wound healing.

Research Products

• [Journal Article] Notochord-derived hedgehog is essential for tail regeneration in Xenopus tadpole. (2014)
  • Author(s): Taniguchi Y, Watanabe K, Mochii M.
  • Journal Title: BMC Dev Biol. Volume: 14 Issue: 1
  • DOI: 10.1186/1471-213x-14-27
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Developmental regulation of locomotive activity in Xenopus primordial germ cells. (2012)
  • Author(s): Terayama K, Kataoka K, Morichika K, Orii H, Watanabe K, Mochii M
  • Journal Title: Dev Growth Differ Volume: 55 Issue: 2 Pages: 217-228
  • DOI: 10.1111/dgd.12018
  • Peer Reviewed
• [Journal Article] Ectopic formation of primordial germ cells by transplantation of the germ plasm: direct evidence for germ cell determinant in Xenopus. (2012)
  • Author(s): Tada H, Mochii M, Orii H, Watanabe K
  • Journal Title: Dev Biol Volume: 371 Issue: 1 Pages: 86-93
  • DOI: 10.1016/j.ydbio.2012.08.014
  • Peer Reviewed
• [Journal Article] Analysis of localization and reorganization of germ plasm in Xenopus transgenic line with fluorescence-labeled mitochondria. (2012)
  • Author(s): Taguchi A, Takii M, Motoishi M, Orii H, Mochii M, Watanabe K
  • Journal Title: Dev Growth Differ Volume: 54 Issue: 8 Pages: 767-776
  • DOI: 10.1111/dgd.12005
  • NAID: 10031136941
  • Peer Reviewed
• [Presentation] 傷表皮で発現するXenopus es1 について (2015)
  • Author(s): 餅井真
  • Organizer: 次世代両生類研究会 第一回会合
  • Place of Presentation: 基礎生物学研究所（愛知県岡崎市）
  • Year and Date: 2015-08-24
  • Invited
• [Presentation] Screening of early signals in Xenopus tadpole tail regeneration using es1:egfp transgenic lines. (2015)
  • Author(s): Sato K, Umesono Y, Mochii M
  • Organizer: The 5th Short-term Student Exchange Program Between College of Natural Sciences in Dong-A University and School of Science in University of Hyogo
  • Place of Presentation: 兵庫県立先端科学技術支援センター（兵庫県赤穂郡上郡町）
  • Year and Date: 2015-08-22
  • Int'l Joint Research
• [Presentation] アフリカツメガエル初期胚におけるES1遺伝子の機能解析 (2014)
  • Author(s): 佐藤健太郎、佐久間哲士、鈴木賢一、山本卓、渡辺憲二、餅井真
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] アフリカツメガエルにおけるChloride intracellular channel 5の発現と機能 (2014)
  • Author(s): 伊藤泰斗、渡辺憲二、餅井真
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] アフリカツメガエルの始原生殖細胞でzygoticに発現する遺伝子の解明 (2014)
  • Author(s): 多田葉瑠、餅井真、梅園良彦、織井秀文
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Xenopus ES1 is expressed in the wound epidermis of regenerating tail and limb bud in tadpole. (2012)
  • Author(s): Yasukawa S, Yoshioka K, Tazaki A, Watanabe K, Mochii M
  • Organizer: 第45回日本発生生物学会年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
• [Presentation] ゼノパス幼生の尾部再生からわかること (2012)
  • Author(s): 餅井真
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学(大阪府)
  • Invited
• [Presentation] Xenopus幼生尾部再生の開始メカニズム (2012)
  • Author(s): 大杉、保地、渡辺、餅井
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学(大阪府)