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Origin and multipotency of Oct60-expressing cells in the adult tissue of Xenopus laevis

Research Project

Project/Area Number 24570240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionRikkyo University

Principal Investigator

KINOSHITA TSUTOMU  立教大学, 理学部, 教授 (30161532)

Co-Investigator(Kenkyū-buntansha) KUBO Hideo  東京都医学総合研究所, ゲノム医科学研究部門, 研究員 (50178034)
MORICHIKA Keisuke  立教大学, 理学部, ポストドクトラルフェロー (30593074)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアフリカツメガエル / 器官形成 / 多能性細胞 / Oct60 / 造血系前駆細胞 / 遺伝子破壊 / 多能性幹細胞 / 変態 / 幹細胞 / 造血
Outline of Final Research Achievements

Oct60 gene is Xenopus paralog of mammalian Oct3/4 gene which encodes a transcription factor of POU family class V. In immunohistochemical analysis, the Oct60-expressing cells were detected in adult gonads, liver, kidney and blood cells. The Oct60-expressing cells were stained with anti-Bmi1 antibody and increased in number by anemic induction, suggesting that the Oct60-expressing cells are hematopoietic precursor-like cells. Cell transplantation experiment indicated that the Oct60-expressing cells are derived from dorsolateral mesoderm of tail-bud stage embryos. Functional analysis of Oct60 showed that the Oct60-expressing cells are multipotent undifferentiated cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (25 results)

All 2015 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (19 results) Remarks (3 results)

  • [Journal Article] Protocadherin-9 involvement in retinal development in Xenopus laevis2015

    • Author(s)
      Izuta Y, Taira T, Asayama A, Kinoshita T, Suzuki S.T.
    • Journal Title

      J. Biochem.

      Volume: 157 Pages: 235-249

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The structure and development of Xenopus laevis cornea.2013

    • Author(s)
      Hu W., Haamedi N., Lee J., Kinoshita T. Ohnuma S.
    • Journal Title

      Exptl. Eye Res.

      Volume: 116 Pages: 109-128

    • DOI

      10.1016/j.exer.2013.07.021

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus ooctyes.2012

    • Author(s)
      Morichika K, Sugimoto M, Yasuda K, Kinoshita T.
    • Journal Title

      Zygote

      Volume: vol.20 Issue: 2 Pages: 1-9

    • DOI

      10.1017/s0967199412000536

    • Related Report
      2012 Research-status Report
  • [Presentation] Functional analysis of Oct60 in adult organ formation of Xenopus laevis2014

    • Author(s)
      伊東直人、佐久間哲史、山本卓、木下勉
    • Organizer
      日本分子生物学会第37回大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] アフリカツメガエルの側線形成におけるOct91の遺伝子発現と機能の解析2014

    • Author(s)
      天川あや、佐久間哲史、山本卓、木下勉
    • Organizer
      日本分子生物学会第37回大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] TALENによる遺伝子破壊個体を用いたOct25の器官形成における機能の解析2014

    • Author(s)
      李宰勲、佐久間哲史、山本卓、木下勉
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Tissue reconstruction of myocardium during Xenopus laevis metamorphosis2014

    • Author(s)
      Kobayashi, M., Sugiura, M., Kinoshita, T.
    • Organizer
      15th International Xenopus Conference
    • Place of Presentation
      Asilomar, California (USA)
    • Year and Date
      2014-08-25 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Localization of Oct25-expressing cells during develoment and regeneration of hindlimb phalanges in Xenopus laevis2014

    • Author(s)
      Lee, J., Shoji, Y., Kinoshita, T.
    • Organizer
      15th International Xenopus Conference
    • Place of Presentation
      Asilomar, California (USA)
    • Year and Date
      2014-08-25 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] アフリカツメガエルの変態期における心筋の組織再構築2014

    • Author(s)
      小林愛、杉浦美紗、木下勉
    • Organizer
      日本発生生物学会2014年度(第47回)年会
    • Place of Presentation
      WINC愛知(愛知県名古屋市)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Oct60 is involved in the PGC formation as a germplasm component2013

    • Author(s)
      Keisuke Morichika, Keigo Shimada, Hideo Kubo, Tsutomu Kinoshita
    • Organizer
      17th International Congress of Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2013 Research-status Report
  • [Presentation] Regulation of red blood cell transition from larval to adult type during anuran metamorphosis2013

    • Author(s)
      Masahiro Yamabuchi, Yui Kawaguchi, Iyo Matsuda, Tsutomu Kinoshita
    • Organizer
      第46回日本発生生物学会
    • Place of Presentation
      くにびきメッセ(島根)
    • Related Report
      2013 Research-status Report
  • [Presentation] Oct60 is involved in the PGC formation as a germplasm component2013

    • Author(s)
      Keisuke Morichika, Keigo Shimada, Hideo Kubo, Tsutomu Kinoshita
    • Organizer
      第46回日本発生生物学会
    • Place of Presentation
      くにびきメッセ(島根)
    • Related Report
      2013 Research-status Report
  • [Presentation] アフリカツメガエルの変態期における成体型心筋形成に関する研究2013

    • Author(s)
      杉浦美紗、森近恵祐、木下勉
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2013 Research-status Report
  • [Presentation] アフリカツメガエルの毛様体辺縁部における細胞の分裂能の解析2013

    • Author(s)
      李宰勲、森近恵祐、木下勉
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2013 Research-status Report
  • [Presentation] アフリカツメガエルの成体型表皮形成におけるOct25/91の役割2013

    • Author(s)
      天川あや、山浦貴史、久保英夫、森近恵祐、木下勉
    • Organizer
      第84回日本動物学会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2013 Research-status Report
  • [Presentation] アフリカツメガエルの変態期における成体型心筋形成に関する研究2013

    • Author(s)
      杉浦美紗、森近恵祐、木下勉
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Related Report
      2013 Research-status Report
  • [Presentation] Oct60 protein is involved in the PGC formation as a germplasm component2012

    • Author(s)
      K.Morichika, K. Shimada, H. Kubo, T.Kinoshita
    • Organizer
      14th International Xenopus Conference
    • Place of Presentation
      Giens Peninsula, France
    • Related Report
      2012 Research-status Report
  • [Presentation] Protein localization and functional analysis of POU-V Oct60 transcription factor in Xenopus laevis2012

    • Author(s)
      Y. Takaichi, K. Shimada, M.Sugiura, H.Kubo, K.Morichika, T.Kinoshita
    • Organizer
      45th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Oct60 expression in adult blood cells of Xenopus laevis2012

    • Author(s)
      F.Kouno, K.Morichika, H.Kubo, T.Kinoshita
    • Organizer
      35th Ann. Meeting Mol. Biol. Society Jap.
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Analysis of regenerative activity in the adult heart of Xenopus laevis2012

    • Author(s)
      T.Yamaura, H.Kubo, K.Morichika, T.Kinoshita
    • Organizer
      35th Ann. Meeting Mol. Biol. Society Jap.
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Analysis of oct25-expressing cells during development of hind limb phalanges in Xenopus laevis2012

    • Author(s)
      Y.Shoji, H.Kubo, K.Morichika, T.Kinoshita
    • Organizer
      35th Ann. Meeting Mol. Biol. Society Jap.
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Study on cardiac tissue remodeling in Xenopus laevis metamorphosis2012

    • Author(s)
      M.Sugiura, K.Morichika, T.Kinoshita
    • Organizer
      35th Ann. Meeting Mol. Biol. Society Jap.
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Remarks] 立教大学 木下研究室

    • URL

      http://www2.rikkyo.ac.jp/web/tkinoshita/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 立教大学 木下研究室

    • URL

      http://www2.rikkyo.ac.jp/web/tkinoshita/

    • Related Report
      2013 Research-status Report
  • [Remarks] 木下研究室ホームページ

    • URL

      http://www2.rikkyo.ac.jp/web/tkinoshita/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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