Origin and multipotency of Oct60-expressing cells in the adult tissue of Xenopus laevis
| Project/Area Number |
24570240
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Rikkyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUBO Hideo 東京都医学総合研究所, ゲノム医科学研究部門, 研究員 (50178034)
MORICHIKA Keisuke 立教大学, 理学部, ポストドクトラルフェロー (30593074)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アフリカツメガエル / 器官形成 / 多能性細胞 / Oct60 / 造血系前駆細胞 / 遺伝子破壊 / 多能性幹細胞 / 変態 / 幹細胞 / 造血 |
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| Outline of Final Research Achievements |
Oct60 gene is Xenopus paralog of mammalian Oct3/4 gene which encodes a transcription factor of POU family class V. In immunohistochemical analysis, the Oct60-expressing cells were detected in adult gonads, liver, kidney and blood cells. The Oct60-expressing cells were stained with anti-Bmi1 antibody and increased in number by anemic induction, suggesting that the Oct60-expressing cells are hematopoietic precursor-like cells. Cell transplantation experiment indicated that the Oct60-expressing cells are derived from dorsolateral mesoderm of tail-bud stage embryos. Functional analysis of Oct60 showed that the Oct60-expressing cells are multipotent undifferentiated cells.
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Report
(4 results)
Research Products
(25 results)
