| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
My laboratory has recently identified the C. elegans male-specific gene spe-45 that is essential for gamete fusion. In order to identify sperm or oocyte factors that associate with SPE-45 protein, I constructed a transgene encodong SPE-45 in which a FLAG-tag is inserted into the cytoplasmic tail domain. As the transgene was expressed in spe-45 mutants, self-fertility of the mutant worms was rescued to about 70% levels of wild-type worms. Currently, by using anti-FLAG antibody, I am trying to identify SPE-45-associating proteins.
I also tried to identify oocyte factors that are indispensable for gamete fusion by different ways. From the database, I selected oocyte-specific genes encoding transmembrane proteins. Then, I disrupted some of the selected genes by the CRISPR/Cas9 method. So far I have obtained five mutant strains, but neither of them were sterile at all. I'll continue to create mutants by the same way.
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