Identification of gamete fusion-related factors that are present on the oocyte surface
Project/Area Number |
24570241
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Developmental biology
|
Research Institution | Setsunan University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 精子 / 卵子 / 配偶子間融合 / 受精 / 線虫 / 免疫グロブリン様タンパク質 / 膜タンパク質 / 免疫グロブリン様ドメイン / 雌雄配偶子間融合 / Ig様タンパク質 / マウス |
Outline of Final Research Achievements |
My laboratory has recently identified the C. elegans male-specific gene spe-45 that is essential for gamete fusion. In order to identify sperm or oocyte factors that associate with SPE-45 protein, I constructed a transgene encodong SPE-45 in which a FLAG-tag is inserted into the cytoplasmic tail domain. As the transgene was expressed in spe-45 mutants, self-fertility of the mutant worms was rescued to about 70% levels of wild-type worms. Currently, by using anti-FLAG antibody, I am trying to identify SPE-45-associating proteins. I also tried to identify oocyte factors that are indispensable for gamete fusion by different ways. From the database, I selected oocyte-specific genes encoding transmembrane proteins. Then, I disrupted some of the selected genes by the CRISPR/Cas9 method. So far I have obtained five mutant strains, but neither of them were sterile at all. I'll continue to create mutants by the same way.
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Report
(4 results)
Research Products
(12 results)